Tehnici utilizate in imunologie - PIM Copy

5. Testul anti-imunoglobulinic (testul Coombs)...............................50
Teste de fază solidă - Solid phase assay.........................................52
1. RIA - Radio Immuno Assay..........................................................52
2. RIST – Radioimmunosorbent test.................................................53
3. RAST – Radioallergosorbent test.................................................54
4. ELISA - Enzyme Linked Immunosorbent Assay.........................56
4.1 ELISA indirectă..........................................................................57
4.2 ELISA sandwich.........................................................................59
4.3 ELISA competitivă.....................................................................60
4.4 ELISPOT.....................................................................................61
5. Chemiluminiscenţa........................................................................62
Imunohistochimia (Corina Cianga, Petru Cianga)..........................65
1. Noţiuni de enzimologie.................................................................67
2. Metode de colorare........................................................................70
2.1 Metoda directă.............................................................................70
2.2 Metoda indirectă în doi paşi........................................................71
2.3 Metoda indirectă în trei paşi........................................................72
3. Controale.......................................................................................73
4. Colorarea de fond (nespecifică sau background)..........................74
5. Contracolorarea.............................................................................77
6. Imunocitochimia...........................................................................77
7. Dubla/tripla coloraţie....................................................................78
Imunofluorescenţa..........................................................................80
1. Imunofluorescenţa directă............................................................82
2. Imunofluorescenţa indirectă.........................................................84
Microscopia confocală....................................................................85
Tissue FAXS....................................................................................86
Citometrie în flux (Flow cytometry)..............................................87
1. Principiu........................................................................................87
2. Sortarea particulelor......................................................................94
Aplicaţii ale citometriei în flux......................................................95
1. Imunofenotiparea.........................................................................95
2. Analiza conţinutului ADN (Ploidia ADN)...................................97