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1027. PRELIMINARY INVESTIGATIONS ON CYTOTOXIC ACTIVITY IN VITRO OF ANEWLY SYNTHESIZED PHOTOSENSITISER ON HUMAN TUMOUR CELL LINESIRadostina Alexandrova 1 , Assen Shulev 2 , Gergana Rashkova 1 , Rodica-M. Ion 31Institute of Experimental Pathology and Parasitology,BAS Acad. G. Bonchev str., bl. 25, Sofia 11132Institute of Mechanics, Bulgarian Academy of Sciences, Sofia, Bulgaria3ICECHIM, Chemical Analysis Department, Bucharest, RomaniaIn the recent years the photodynamic therapy of cancer is a field of intensive research.The aim of the present study was to evaluate the in vitro cytotoxic activity of a newlysynthesized photosensitiser on two human tumour cell lines – 8-MG-BA (glioblastoma) andHepG2 (hepatocellular carcinoma). The effect of the compound on cell viability was studiedusing Neutral red cytotoxicity assay. He-Ne laser at 632.8 nm was used at irradiance 40mW/cm 2 . The experiments were performed at radiant exposures varying from 2 to 32 J/cm 2 .The photosensitiser was applied at a concentration of 25 µg/ml. 48 h after the irradiation it wasfound to reduce significantly the viability of human glioblastoma 8-MG-BA cells in a dosedependentmanner. At the same time this compound had no significant cytotoxic effect onhepatocellular carcinoma cells HepG2. Without irradiation the photosensitizer did not affectthe viability of both tumour cell lines.IrradiationCell viabilityJ/cm 2 (% of a control)4 59.21 ± 2.678 44.10 ± 2.9516 36.57 ± 1.9132 30.67 ± 1.3964 14.43 ± 0.87