Biomasa este un termen generic care cuprinde o ... - ICECHIM

932. C. M. Ghiciuc, D. Belei, C. E. Lupuşoru, E. Bâcu, I. M. Antonesi, O. Jerca, R. Lupuşoru, A. Couture,P. Grandclaudon, Ann. Pharm. Fr., 62, 43-48, (2004);3. D. Belei, C. E. Lupuşoru, C. M. Ghiciuc, E. Bâcu, I. Antonesei, L. Tartau, A. Couture, P. Grandclaudon,Terapeutică, Toxicologie, Farmacologie clinică, VIII , 97-101, (2004);4. E. Bâcu, D. Belei, G. Nowogrocki, A. Couture, P. Grandclaudon, Org. Biomol. Chem., 1, 2377-2382, (2003).2. APOPTOTIC MECHANISMS IN EXPERIMENTAL PHOTODYNAMIC THERAPYWITH SYNTHETIC PORPHYRINSMonica Neagu 1 , Gina Manda 1 , Carolina Constantin 1 ,Eugen Radu 1 ,Rodica-Mariana Ion 21. “Victor Babes”, National Institute, Immunology Dept, Bucharest, Romania2. INCD CP-ICECHIM, Bucharest, RomaniaThe aim of the study was to determine the molecular mechanisms that underlay theapoptotic process developed after laser activation of synthetic porphyrins loaded tumorlymphoblastic cell line K562.We have synthetized 5,10,15,20-tetra-1-naphthyl-porphyrin and 5,10,15,20-tetra-psulphonate-phenyl-porphyrin.The porphyrins were used to load K562 cell line in the 5-250microg/ml range and study the effect registered on the functionality of tumor cells.Methods. Lymphoblastic cell line K562 (ECACC) maintained in culture as stated bythe supplier.Dye loading was performed for 24h in the mentioned concentration range for bothporphyrins at 2x10 5 cells/mL. Cell viability was assessed with Cytotox96 Non-RadioactiveCytotoxicity Assay kit and Trypan Blue exclusion test in order to establish the non-toxicconcentration of photosensitizers.Activation was done by irradiation after removal of excess dye and recounting cellsuspensions. 2x10 5 cells/mL suspensions loaded with established non-toxic concentration ofporphyrins were irradiated with He-Ne laser 30 mW, λ=632,8nm, 25 0 C, total irradiation time =50 min, in O2 saturated solution.Cell proliferation was assessed with CellTiter 96AQueous One Solution CellProliferation kit. Early apoptotic events were determined by flow-cytometry with annexin V-FITC labeling.Caspase 3 activity was assessed by the colorimetric method with CaspACE AssaySystem (Promega) and late apoptotic events measured by DeadEnd TUNEL System(Promega).