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EVALUATION OF FOUR SORGHUM HYBRIDS THROUGH THE ...

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Moisture Content:<br />

The moisture contents of the starches and flours were determined using an<br />

infrared moisture analyzer, IR-100 Power Module (Denver Instrument Company, Arvada<br />

CO). Between 2 and 3 grams of starch or flour were placed on the machine and<br />

measurements taken.<br />

Crude Protein Content:<br />

The crude protein of each flour was determined using a LECO FP-528 instrument<br />

(Leco Corporation, St. Joseph, MI). The AACC approved method 46-30 (10 th edition)<br />

was followed.<br />

Ash Content:<br />

The ash content was found using an adoption of the AACC approved method 08-<br />

01. Modifications were as followed: 3 to 4 g of flour, 21 hours at 1000 o F, cool for 1 hour<br />

in desiccator and weigh.<br />

Statistical Design<br />

Four treatments of sorghum flour were evaluated for grain and flour<br />

characterizations. For grain characterization, two replications were performed. For flour<br />

characterization, two replications were performed for particle size distributions, total<br />

starch, starch damage, amylose content, and pasting properties. Triplicate readings were<br />

taken for moisture, protein, and ash content.<br />

All data were analyzed using SAS, Software Release 9.1 (SAS, Institute Inc.,<br />

2003). When treatment effects were found significantly different, the least square means<br />

with Tukey-Kramer groupings were used to differentiate treatment means. A level of<br />

significance was observed at α = 0.05.<br />

54

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