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EVALUATION OF FOUR SORGHUM HYBRIDS THROUGH THE ...

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values. Calibration was performed before each use using buffer solutions of pH 4.0 and<br />

7.0. The AACC method 02-52 was followed according to the flour procedure.<br />

Water Activity<br />

Water activity measurements were determined using an AquaLab water activity<br />

meter (Model Series 3, Decagon Devices Inc., Pullman, WA 99163). The instrument was<br />

standardized prior to experimental readings. The tortilla samples were broken up into<br />

small pieces and placed into disposable sample cups for AquaLab (WP4, Decagon<br />

Devices, Inc. Pullman WA 99163). These cups were filled about halfway and placed into<br />

the measuring chamber. The dial was turned to read and the results recorded from the<br />

digital display on the instrument.<br />

Moisture Content<br />

The moisture content of each tortilla sample was obtained using the AACC<br />

method 44-40. The method calls for 2 grams of sample. Instead, 5 grams of each sample<br />

was used as this provided better readings. The percentage of moisture and volatile matter<br />

were calculated using the following equation.<br />

% Moisture = [Wet Weight (g) – Dry Weight (g)] X 100<br />

Wet weight (g)<br />

Sensory Analysis<br />

Descriptive Analysis<br />

Seven panelists took part in a descriptive analysis of the sorghum flour tortillas.<br />

The panelists met once a week for 2 to 3 hours. A total of 4 sessions were held for<br />

training and development of attributes.<br />

The first session consisted of tasting a sorghum flour tortilla made with<br />

commercial flour and determining attributes in terms of appearance, texture in the hand,<br />

88

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