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M i c r o s c o p y Fa c i l i t i e s Cancer Research in Glasgow The Beatson Advanced Imaging Resource (BAIR) Cancer Research UK is a major funder of biomedical research in Europe. In 2006/2007 they spent £315 million to support over 500 research groups in the UK through a variety of funding mechanisms including research institutes, clinical centers, and grants. The Beatson Cancer Research Institute is one of four corefunded CR-UK research institutes. Recently, CR-UK invested £22 million towards a new building for the Beatson (Fig. 1) located on the University of Glasgow campus and scheduled for occupation in December of 2007. This expansion will double the size of the institute and is associated with substantial new investment in research infrastructure. New Facility of the Beatson Cancer Research Institute The Beatson Advanced Imaging Resource (BAIR) is the new imaging facility of the Beatson Cancer Research Institute. The Bair occupies 280 m 2 of space in eleven rooms located primarily in the basement of the new building, with two additional rooms located in the biomedical unit to facilitate mouse in vivo imaging (see below). Smooth operation is ensured by two full time scientific officers who train users, assist with advanced applications, troubleshoot equipment, and perform quality control. Staff also maintain an internal website which includes tutorials, operating procedures, protocols, and equipment specifications in addition to the scheduling database through which both equipment and assistants are booked. The BAIR is managed by Dr Kurt I. Anderson, who brought to Glasgow the experience of having set up the light mi- 44 • G.I.T. Imaging & Microscopy 4/2007 croscopy facility of the Max Planck Institute for Cell Biology and Genetics in Dresden. Dr. Anderson also leads a research group at the Beatson Institute, which investigates actin dynamics in cell migration using advanced imaging techniques including TIRF, FRAP, photo-activation/ -switching (PA/PS), and FLIM-FRET. The rational behind this joint appointment is to promote the use of advanced imaging techniques among Beatson research groups by introducing opportunities for substantive collaborations which might not be possible within the context of a service alone. Imaging Systems The imaging systems are roughly grouped according to applications (Fig. 2). At the south end of the facility (room 122) are three systems for medium throughput, high content, long term time lapse microscopy consisting of a Nikon TE2000 stand with Perfect Focus, ASI stage with linear Fig. 1: The new Beatson Institute for Cancer Research in Glasgow encoder, Sutter illumination system, and okolab incubator, all driven by Meta- Morph software. BAIR staff maintain FACS analyzers throughout the building, in addition to a BD FACSAria in room 123 with 561 nm diode laser for use with red fluorescent proteins. Three confocal microscopes are housed in room 124, including a Leica TCS-SP2 with AOBS and Olympus FV1000 with 405 nm diode and SIM scanner. The latter system is especially powerful for FRAP and the use of PA/PS probes. A second TCS-SP2 is fitted with a Becker and Hickl module (SPC- 730) and pulsed IR laser for time domain detection of FLIM-FRET. A small room (room 125) is centrally located for the placement of microscope laser modules, which promotes operational stability of the equipment and a more comfortable user environment. A workshop is located in room 126 along with a demo space for testing of new equipment. Room 127 houses several custom systems for live cell imaging. A system for TIRF microscopy has been built based on a Nikon TE2000 stand and custom condensor incorporating 405 and 473 nm diode lasers, which enables FRAP and PA/PS in TIRF. Integration of a 561 nm diode laser is currently under way, which will enable simultaneous GFP/RFP imaging in conjunction with an Optical Insights Dual- View and Roper 512 x 512 EMCCDFT
camera. A frequency domain system for FLIM-TIRF is under development together with Lambert Instruments based on their LIFA system, which will offer enhanced time resolution compared to the TCSPC approach and sensitive detection of protein-protein interactions at the cell surface due to the optical sectioning of TIRF. A LIFA system coupled to a Yokogawa CSU22 spinning disk scanhead is also planned. Room 130 houses several upright stands for imaging fixed specimens, including histological stains, immunofluorescence, and FISH. Offline Beatson Advanced Imaging Resource (BAIR) rm 122 rm 123 rm 124 rm 125 / rm 126 rm 127 rm 130 rm 129 rm 131 C D E E Fig. 2: Floor plan of the Beatson Advanced Imaging Resource (see text for details) � �� B A workstations for image processing and analysis are located in room 129, including Volocity, MetaMorph, the Olympus and Leica confocal packages, and of course ImageJ. Finally, staff share office space in room 128. Focus at the Beatson rm 128 The development and use of murine cancer models to better understand and treat human disease is an important focus at the Beatson. Mouse in vivo imaging involves a wide range of magnification, sen- A 2 m M i c r o s c o p y Fa c i l i t i e s sitivity, and resolution depending on the experiment. For imaging groups of fluorescent cells down to the level of single cells an Olympus OV100 is used. With this system tumor progression can be monitored in a variety of tissues, including skin, colon and pancreas. For high resolution imaging of cells and sub-cellular structure is currently used an Olympus FV1000 confocal microscope. However a multi-photon TRIM scope from LaVision Biotec is under installation. This system incorporates both a Coherent Chameleon laser for excitation of GFP and an APE optical parametric oscillator for excitation of red fluorescent proteins. Finally, an Ivis 50 is used for detection of chemi-luminescence. The Beatson Institute looks forward to introducing the BAIR and their new research facility to the imaging community by hosting the European Light Microscopy Initiative meeting in Glasgow together with the University of Strathclyde in 2009. Contact: Dr. Kurt I. Anderson Beatson Institute for Cancer Research Glasgow, Scotland, United Kingdom Tel.: +44 141 330 2864 Fax: +44 141 942 6521 k.anderson@beatson.gla.ac.uk �� G.I.T. Imaging & Microscopy 4/2007 • 45
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