The vagus nerve as a modulator of intestinal inflammation - TI Pharma

More documents

Recommendations

Info

RESULTS Cholinergic agonists enhance phagocytosis in macrophages Vagal activity enhances macrophage phagocytosis We first tested whether ACh, the main neurotransmitter of the vagus nerve, or nicotine, that activates nAChRs, affected macrophage endo- and phagocytosis. In PMF (Fig. 1A-D), and spleen macrophage Mf4/4 cells (not shown), ACh as well as nicotine significantly (p
Chapter 5 Figure 1 Cholinergic agonists enhance macrophage phagocytosis. A) Uptake of FITC-Zymosan (green particles) in peritoneal macrophages grown on glass slides. Cells were pretreated with nicotine (1 µM) or ACh (1 µM) and incubated at 37 ºC or on ice where indicated. Cells were stained using phalloidin (red), and DaPi (blue). Scale bar: 10 µm. B) Time course of uptake by PMF pretreated with vehicle, acetylcholine or nicotine. C) Nicotine (triangles) as well as acetylcholine (circles) increases uptake of FITC-Zymosan in PMF. Data are given as the percentage increase in the number of FITC-Zymosanpositive cells, compared to medium treatment (100%). D) The effect of nicotine on the phagocytosis of E.faecium bacteria, Zymosan, opsonised RBC and AcLDL by PMF. Data shown are averages +/- SEM of 3-5 independent experiments in triplicate. Asterisks indicate significant difference (P
Page 1 and 2:
The vagus nerve as a modulator ofin
Page 3 and 4:
The vagus nerve as a modulator of i
Page 5 and 6:
Promotiecommissie Promotores: Prof.
Page 7 and 8:
Table of contents Chapter 1 Introdu
Page 9 and 10:
Introduction and outline The aim of
Page 11 and 12:
Introduction and outline Hence, the
Page 13 and 14:
Introduction and outline 18. van de
Page 15 and 16:
Chapter 2 ABSTRACT The cholinergic
Page 17 and 18:
Chapter 2 neurotransmitter ACh with
Page 19 and 20:
Chapter 2 THE VAGUS NERVE AND CHOLI
Page 21 and 22:
Chapter 2 nicotine treatment impair
Page 23 and 24:
Chapter 2 Figure 1. Hypothetical sc
Page 25 and 26:
Chapter 2 unclear whether this hybr
Page 27 and 28: Chapter 2 reduction of NF-κB activ
Page 29 and 30: Chapter 2 CONCLUDING REMARKS The hy
Page 31 and 32: Chapter 2 34 17. The FO, Boeckxstae
Page 33 and 34: Chapter 2 55. Moser N, Mechawar N,
Page 35 and 36: Chapter 2 93. Shafique S, Dalsing M
Page 37 and 38: Chapter 3 ABSTRACT Acetylcholine re
Page 39 and 40: Chapter 3 by bowel manipulation 12
Page 41 and 42: Chapter 3 for preparation of whole
Page 43 and 44: Chapter 3 Figure 1. Nicotine attenu
Page 45 and 46: Chapter 3 Figure 2. Inhibition of m
Page 47 and 48: Chapter 3 macrophage cell lysates s
Page 49 and 50: Chapter 3 reports 2 . We next incub
Page 51 and 52: Chapter 3 Figure 5. Perioperative e
Page 53 and 54: Chapter 3 Figure 7. Stimulation of
Page 55 and 56: Chapter 3 Activation of the STAT3 c
Page 57 and 58: Chapter 3 REFERENCE LIST 60 1. Trac
Page 59 and 60: 4 CHAPTER Deciphering STAT3 signali
Page 61 and 62: INTRODUCTION Deciphering STAT3 sign
Page 63 and 64: Deciphering STAT3 signaling in chol
Page 69 and 70: DISCUSSION Deciphering STAT3 signal
Page 71 and 72: REFERENCES Deciphering STAT3 signal
Page 73 and 74: Chapter 5 ABSTRACT Background and A
Page 75 and 76: Chapter 5 METHODS Reagents and anti
Page 77: Chapter 5 described previously 6 .
Page 81 and 82: Chapter 5 Figure 3 Cholinergic agon
Page 83 and 84: Chapter 5 we analyzed whether nicot
Page 85 and 86: Chapter 5 Zymosan induced formation
Page 87 and 88: Chapter 5 marker CD11 c (Fig. 7E),
Page 89 and 90: Chapter 5 Our data demonstrate that
Page 91 and 92: Chapter 5 REFERENCE LIST 96 1. Boro
Page 93 and 94: Thesis E.P.M. van der Zanden The va
Page 95 and 96: Summary and conclusions The goal of
Page 97 and 98: Summary and conclusions whether the
Page 99 and 100: Summary and conclusions exposure co
Page 101 and 102: REFERENCE LIST Summary and conclusi
Page 103 and 104: Nederlandse samenvatting
Page 105 and 106: Chapter 7 van STAT3 in de ‘cholin
Page 107 and 108: Chapter 7 De bevinding dat nicotine
Page 109 and 110: Chapter 7 macrofagen aan te tonen (
Page 111 and 112: Chapter 7 18. Yu Z, Zhang W, Kone B
Page 113 and 114: Shizuo Akira Department of Host Def
Page 115 and 116: List of publications
Page 117 and 118: Dankwoord
Page 119 and 120: om de ene keer olijfolie te drinken
Page 121: Curriculum Vitae Curriculum Vitae E
show all

The vagus nerve as a modulator of intestinal inflammation - TI Pharma

Create successful ePaper yourself

Delete template?

Save as template?