initial characterization of crude extracts from phyllanthus amarus

values (0.13; 0.80; 0.88) as fractions 2, 7, and 8 (0.14; 0.83; 0.91) from detection method
I (Appendix D, Tables D.1 and D.2). Therefore these fractions/compounds are most
probably the same and detection method II can be eliminated for the determination of
samples from location PD.
Table 4.2 Presence/absence of fractions in crude P. amarus extracts detected by
UV-365 nm and Dragendorff (DRG) reagent (detection method II).
Cross-reference with Figure C.5 and Table D.2
Fraction
Extraction\
Location
A1
B1
C1
A2
B2
C2
A3
B3
C3
1 2 3 Total
-
-
+
-
+
+
-
-
-
Extraction: A=50% MeOH in H2O; B= 99% methanol; C= 50% MeOH in CHCl3
Location: 1= PD; 2= PN; 3= PZ
The use of mobile phase chloroform/methanol= 9:1 resulted in some compounds
that were partially separated. The two last compounds were still blended (Figure C.4).
The mobile phase chloroform/methanol= 98:2 (Figure C.6) gave basically the same
results as mobile phase 95:5 (Figure C.5).
-
+
+
-
+
+
-
+
+
-
+
+
-
+
+
-
+
+
0
2
3
0
3
3
0
2
2
From extract A of location PD and PN (Table 4.3 and Figure C.8), only one
fraction (number 7) became visible. Fraction 3 was detected only in extract C. This is an
32