initial characterization of crude extracts from phyllanthus amarus

in Q. amara extract that were detected by sulfuric acid are the terpenoids and some
alkaloids.
Table 4.6 Presence/absence of fractions in crude Q. amara extracts detected by
5% ethanolic H2SO4 reagent (detection method III). Cross-reference
with Figure C.13 and Table D.6
Fraction
Extraction
A
B
C
1 2 3 4 5 6 Total
-
+
+
-
+
+
-
+
+
Extraction: A=50% MeOH in H2O; B= 99% methanol; C= 50% MeOH in CHCl3
-
+
+
The mobile phases 9:1 and 98:2 (Figure C.13) eluted fewer compounds than
95:5. Since the mobile phase of chloroform/methanol= 95:5 gave a good separation and
eluted more compounds than the other mobile phases, the plates from this mobile phase
were used for analysis.
Table 4.7 Presence/absence of fractions in crude Q. amara extracts detected by
sulfuric acid (5% ethanolic H2SO4) reagent and UV-365nm (detection
method IV). Cross-reference with Figure C.14 and Table D.7
Fraction
Extraction
A
B
C
1 2 3 4 Total
+
+
+
-
+
+
+
+
+
Extraction: A=50% MeOH in H2O; B= 99% methanol; C= 50% MeOH in CHCl3
+
+
+
Only fraction 2 from extract A (Table 4.7 and Figure C.14) was not detected by
this method. Fewer fractions/compounds become visible with detection method IV than
with the other three detection methods. Spraying the plate with sulfuric acid reagent first
36
-
+
+
3
4
4
-
-
+
0
5
6