HPLC Analysis of Biomolecules Technical Guide - Interscience

More documents

Recommendations

Info

10 HPLC Analysis of Biomolecules The length of the bonded phase alkyl chain can also influence the selectivity of the separation, however not to the same extent as bonded phase functionality. For small molecules (
Figure 11: Retention of nucleosides on C18 vs. PGC Figure 12: Amino acids on PGC Hypercarb, 5 µm, 100 x 1.0 mm Part No.: 35005-101030 Eluent: A: Ammonium acetate 5 mM, pH 9.6 + 2% ACN B: Ammonium acetate 5 mM, pH 9.6 + 80% ACN Gradient: 5 to 40% B in 80 min Flow Rate: 50 µL/min Detection: +ESI Data courtesy of Nana Kawasaki, National Institute of Health Science, Tokyo, Japan Figure 13: Oligosaccharides from a glycoprotein on PGC 11
Page 1 and 2: c h r o m a t o g r a p h y HPLC An
Page 3 and 4: Table of Contents HPLC Modes for Bi
Page 5 and 6: HPLC Modes for Biomolecule Separati
Page 7 and 8: efficiency is observed as the large
Page 9: Figure 7: Effect of flow rate on gr
Page 13 and 14: Figure 15: Effect of pH on RP-HPLC
Page 15 and 16: Effect of Temperature on RP-HPLC Re
Page 17 and 18: Maintaining Constant Linear Velocit
Page 19 and 20: Hypercarb, 5 µm, 30 x 3 mm Part No
Page 21 and 22: Organic/Aqueous Solvent Ratio for R
Page 23 and 24: Ion Exchange Chromatography Basic P
Page 25 and 26: Column Type of Ion exchange Particl
Page 27 and 28: Figure 46: Effect of decreasing ion
Page 29 and 30: Figure 51: Molecular weight calibra
Page 31 and 32: Figure 55 shows how temperature con
Page 33 and 34: Proteomics Proteomics and LC/MS n P
Page 35 and 36: Figure 61: 2-D LC/LC-MS 2 tryptic d
Page 37 and 38: Column Cleaning If some components
Page 39 and 40: Common Buffer Systems Used in HPLC
Page 41 and 42: 5 µm BioBasic PEEK Columns Descri
Page 43 and 44: 3 µm & 5 µm Hypercarb Columns Des
Page 45 and 46: Finnigan Surveyor HPLC Systems The
Page 47 and 48: Proteomics Capillary LC/MS ........

HPLC Analysis of Biomolecules Technical Guide - Interscience

Create successful ePaper yourself

Delete template?

Save as template?