HPLC Analysis of Biomolecules Technical Guide - Interscience
HPLC Analysis of Biomolecules Technical Guide - Interscience
HPLC Analysis of Biomolecules Technical Guide - Interscience
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46<br />
Appendicies<br />
Appendix 4: Index<br />
Alkyl Chain Length<br />
Reversed Phase ...........................................9<br />
Amino Acids<br />
Properties...................................................38<br />
Anion Exchange<br />
Capacity .....................................................25<br />
Structure ....................................................25<br />
BetaBasic Columns<br />
Description...................................................5<br />
Ordering Tables..........................................40<br />
BioBasic Columns<br />
Description...................................................5<br />
Ion Exchange .............................................23<br />
Ordering Tables<br />
Reversed Phase ...........................................6<br />
Size Exclusion ...........................................28<br />
Buffers<br />
Common.....................................................39<br />
General Recommendations .......................36<br />
Capillary Columns<br />
LC/MS........................................................17<br />
Proteomics .................................................33<br />
Cation Exchange<br />
Capacity .....................................................25<br />
Structure ....................................................25<br />
Column Care<br />
Cleaning.....................................................37<br />
Protection...................................................36<br />
Storage ......................................................37<br />
Column Length<br />
Ion Exchange .............................................24<br />
Reversed Phase ...........................................8<br />
Size Exclusion ...........................................30<br />
Direct Serum Injection<br />
Size Exclusion ...........................................31<br />
Efficiency<br />
Reversed Phase ...........................................6<br />
ESI<br />
Proteomics .................................................33<br />
Reversed Phase LC/MS.............................17<br />
Filters<br />
Column Protection .....................................36<br />
Flow Rate<br />
Ion Exchange .............................................24<br />
Reversed Phase ...........................................8<br />
Size Exclusion ...........................................30<br />
vs. ID..........................................................39<br />
Gradient<br />
pH...............................................................27<br />
Reversed Phase .........................................15<br />
Salt.............................................................27<br />
Guard Columns<br />
Column Protection .....................................36<br />
<strong>HPLC</strong> Solvents<br />
Properties...................................................38<br />
Hypercarb Columns<br />
Description...................................................5<br />
Interaction Types .........................................9<br />
LC/MS........................................................18<br />
Microscale SPE..........................................35<br />
Ordering Tables..........................................43<br />
Polar Retention..........................................10<br />
Internal Diameter<br />
RP-LC/MS ..................................................16<br />
vs. Flow Rate .............................................39<br />
Ion Exchange<br />
Capacity .....................................................25<br />
Flow Rate...................................................24<br />
Ionic Strength ............................................26<br />
Mechanisms ..............................................23<br />
Mobile Phase.............................................26<br />
Particles .....................................................24<br />
pH...............................................................26<br />
Phases........................................................25<br />
Temperature...............................................24<br />
Ion Pairing<br />
TFA.............................................................14<br />
Isocratic<br />
Reversed Phase .....................................8, 15<br />
LC/MS<br />
Column Selection ......................................16<br />
ESI..............................................................17<br />
Ion Exchange .............................................27<br />
Ionic Additives...........................................20<br />
Mobile Phase.............................................20<br />
Nanospray..................................................18<br />
Organic/Aqueous Ratio .............................21<br />
Packing Materials......................................18<br />
Reversed Phase .........................................16<br />
Size Exclusion ...........................................31<br />
Linear Velocity<br />
Definition .....................................................8<br />
LC/MS........................................................17<br />
Reversed Phase ...........................................8<br />
Mechanisms<br />
Hypercarb/PGC .....................................9, 10<br />
Ion Exchange .............................................23<br />
Polar Retention..........................................10<br />
Reversed Phase ...........................................9<br />
Size Exclusion ...........................................28<br />
Microscale SPE<br />
Sample Clean-Up.......................................35<br />
Mobile Phase<br />
Additives....................................................12<br />
Ionic Modifiers...........................................13<br />
Ionic Strength ............................................26<br />
LC/MS........................................................20<br />
Organic Modifier........................................14<br />
Organic/Aqueous Ratio .............................21<br />
pH...............................................................13<br />
pH for Ion Exchange ..................................26<br />
RP-<strong>HPLC</strong> .....................................................12<br />
SEC.............................................................30<br />
Moleclar Weight<br />
Column Selection ......................................29<br />
Common <strong>Biomolecules</strong>..............................38<br />
Size Exclusion ...........................................29<br />
Nanospray<br />
Reversed Phase LC/MS.............................19<br />
Particle Size<br />
Ion Exchange .............................................24<br />
Reversed Phase ...........................................6<br />
Size Exclusion ...........................................30<br />
pH<br />
Gradient .....................................................27<br />
Ion Exchange .........................................26<br />
Mobile Phase.........................................13<br />
pH Stability<br />
BetaBasic Columns....................................19<br />
BioBasic Columns......................................19<br />
General Recommendations .......................36<br />
Hypercarb/PGC Columns ...........................10<br />
Polar Retention<br />
Hypercarb/PGC ..........................................10<br />
Pore Size<br />
Ion Exchange .............................................24<br />
Reversed Phase ...........................................7<br />
Size Exclusion ...........................................29<br />
Porous Graphitic Carbon<br />
Interaction Types .........................................9<br />
LC/MS........................................................18<br />
Microscale SPE..........................................35<br />
Polar Retention..........................................10<br />
Preparative Chromatography<br />
Calculations ...............................................39<br />
Pressure<br />
General Recommendations .......................36<br />
vs. Flow Rate ...............................................8<br />
Protein Structure<br />
3-D Shape..................................................38
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