HPLC Analysis of Biomolecules Technical Guide - Interscience

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Appendicies
Appendix 3: Instruments for HPLC of Biomolecules
Integrated Finnigan ProteomeX LTQ Workstation
The Finnigan ProteomeX LTQ is a fully integrated, multidimensional
capillary LC/MS n system based on the ultra-sensitive Finnigan LTQ
linear ion trap mass spectrometer, Micro AS autosampler, BioBasic
capillary columns, and BioWorks software. The ProteomeX LTQ is
designed and optimized for LC-MS/MS experiments required for
proteomics, such as high throughput protein identification, shotgun
sequencing of complex samples, and differential protein quantitation
using ICAT reagents.
The ProteomeX LTQ
workstation is a comprehensive
package that
includes all the elements
necessary for sophisticated
proteomics analyses –
HPLC, MS, customized
software, columns,
standards, methods, and
BioWorks post-acquisition
protein analysis software.
The system is installed
to meet multidimensional
LC-MS/MS performance
criteria so that a researcher will immediately be able to run
meaningful experiments.
Operation of this powerful system has been greatly simplified
without compromising performance. The ProteomeX LTQ starts with
the simplicity and ruggedness of the LTQ with an ESI probe and
builds on it with an exclusive programming interface customized for
proteomics researchers. The ProteomeX LTQ facilitates easy method
set-up and execution, plus automatic identification of peptides and
proteins with the proven BioWorks software package. The ProteomeX
LTQ workstation permits automated detection and identification of
peptides from hundreds or thousands of proteins in a single sample.
The ProteomeX LTQ workstation allows immediate access to
automated, on-line, multidimensional LC-MS/MS, coupling ion
exchange and reversed-phase chromatography to enable exquisite
resolution of large numbers of peptides for detection by the ultrasensitive
LTQ. This can easily be combined with ICAT methodologies
for quantitation of differentially expressed proteins.
• High-Throughput Protein ID
• Maximum Sequence Coverage
• Phosphorylation Site Mapping
• Multidimensional Protein ID (MudPIT)
• Protein quantitation with ICAT
• BioWorks protein analysis software with TurboSEQUEST Finnigan LTQ Linear Ion Trap LC/MS/MS n
The newest addition to the Finnigan ion trap product line is the LTQ –
a high-performance, 2-dimensional (2-D) linear ion trap mass
spectrometer. The LTQ redefines performance for the metabolic and
proteomics arena. Superior sensitivity and fast scanning produces
excellent peak shape, resulting in quantitation over a wide linear
dynamic range. Unmatched MSn sensitivity rapidly facilitates both
metabolic and proteomic structural elucidation. Combining patented
technology from the field-proven LCQ series with advanced designs
in linear trap, ion source, optics, and detector technology, the LTQ
delivers exceptional performance as the gold standard mass spectrometer.
The segmented linear trap with radial ejection and dual
detection system produces the highest overall detection efficiency
resulting in unparalleled sensitivity. These capabilities, combined
with rapid scanning available through state-of-the-art electronics,
result in information rich data collection at breakthrough speed.
The LTQ features the revolutionary Ion Max source, designed
for ruggedness and ease of use. Simple tool-less switching of
ionization probes, a wide aperture titanium skimmer, a large volume
ion transfer tube, and x,y,z positioning elements make the Ion Max
the most robust commercially available ion source. An ultra hightemperature,
self-cleaning ceramic APCI heater greatly diminishes
background noise, resulting in dramatic APCI mode performance
advances and greater ease of use. The LTQ may also be equipped
with APCI, LCQ NanoSpray, APCI/APPI, and AP-MALDI ion sources.
• Ultra-high sensitivity and resolution at fast scan speeds
• Industry’s highest trapping and detection efficiencies
• Unmatched MSn quality on low abundance ions
• Extraordinary performance with ease-of-use