HPLC Analysis of Biomolecules Technical Guide - Interscience
HPLC Analysis of Biomolecules Technical Guide - Interscience
HPLC Analysis of Biomolecules Technical Guide - Interscience
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22<br />
<strong>HPLC</strong> <strong>Analysis</strong> <strong>of</strong> <strong>Biomolecules</strong><br />
Column 0.01" ID Tube 0.005" ID Tube 0.003" ID Tube<br />
ID (mm) Volume (µL) Flow (mL/min) Max L (cm) Max Vol (µL) Max L (cm) Max Vol (µL) Max L (cm) Max Vol (µL)<br />
4.60 1080 1.0000 35.314 17.894 565.020 71.575 4359.726 197.820<br />
4.00 817 0.8000 28.051 14.214 403.815 51.154 3115.859 142.095<br />
3.00 459 0.5000 12.777 6.474 204.432 25.897 1577.404 71.935<br />
2.10 204 0.2000 6.310 3.197 100.954 12.789 778.965 35.524<br />
1.00 51.1 0.0500 1.577 0.799 25.238 3.197 194.741 8.881<br />
0.50 12.8 0.0120 0.411 0.208 6.573 0.833 50.714 2.313<br />
0.30 4.59 0.0040 0.160 0.081 2.555 0.324 19.718 0.899<br />
0.18 1.65 0.0014 0.057 0.029 0.920 0.117 7.098 0.324<br />
0.10 0.511 0.0004 0.020 0.010 0.315 0.040 2.434 0.111<br />
Calculations based on 100 mm columns, N = 10,000, Porosity = 0.65, k' = 1. Instrumental variance
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