download/files/Proteomics brochure 7-08-04.pdf

CCD image of protein digest
with α-cyano matrix
Analysis of a four-peptide mixture,
200 amol each, with α-cyano matrix
The Perfect System for High-
Throughput Protein Analysis
The Finnigan vMALDI ion source is
completely optimized to harness the
incredible sensitivity of the Finnigan
LTQ for high-throughput MALDI-
MS/MS analysis. The Finnigan
vMALDI ion source utilizes:
• Crystal Positioning System (CPS) to automatically target
the optimum sampling pattern
• Automated Spectral Filtering (ASF) for real-time monitoring
of spectral quality
• Automatic Gain Control (AGC )
to determine the optimum
number of laser shots for the
best possible sensitivity
Angiotensin I fragment 1-7
Bradykinin
Angiotensin I
Neurotensin
The system is designed to allow
walk-away automation during data
acquisition and has the intelligence
to change experimental conditions
to provide the best possible outcome:
First, the CPS performs a
digital analysis of the sample spot
image to rapidly identify crystals
with good signal-to-noise potential.
CPS also remembers the ablated
path, so that only fresh crystals are
interrogated. Then, the Finnigan
vMALDI ion source begins sampling,
using ASF for real-time monitoring
of spectral quality. When high
quality spectra are detected, the
Finnigan vMALDI ion source uses
AGC to determine the optimum
Automated sampling pattern
mapped with CPS
MS/MS of 50 fmol myoglobin digest,
precursor is the T2 fragment at m/z 1606.9
number of laser shots to achieve
the highest possible sensitivity
without sacrificing spectral quality.
Data Dependent MS/MS is immediately
performed on all precursor
ions, giving MS/MS spectra to identify
all peptides that are present.
Poor signal: Move to different crystal
CPS
ASF
AGC
Good Signal
Excellent Quality Spectra
Examine Spectra
Optimize Laser
CPS, ASF, and AGC allow automated acquisition
of high quality MS and MS/MS spectra
MS 3 of 50 fmol myoglobin digest,
precursor is at m/z 1192.7
Move to next sample: Repeat process
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