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Baden-Württemberg - Lichens of Wales

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habitat (to facilitate determination), date and<br />

collector.<br />

Without any doubt impending in the<br />

consequences <strong>of</strong> the precipitous increase in the<br />

number <strong>of</strong> those interested in lichens is a further<br />

danger to these already richly collected<br />

organisms: is the decimation and extirpation by<br />

lichenologists. In collecting lichens one ought to<br />

consider that many lichens are rare and<br />

endangered. In many cases the collection <strong>of</strong> only<br />

five to ten specimens would have the result <strong>of</strong><br />

extirpation <strong>of</strong> the species in Germany. One<br />

collection <strong>of</strong> rare species is without justification,<br />

especially so, when the lichen already is known<br />

and documented from the region. Occasionally<br />

one can in a certain region collect species<br />

endangered in other regions, where they are still<br />

frequent, without an aggravating result being<br />

“provided.”<br />

A completely removed specimen growns no<br />

longer. The decimation <strong>of</strong> a population can lead<br />

to the endangering <strong>of</strong> the rest <strong>of</strong> the population.<br />

A resettling <strong>of</strong> a species on a locality, on which it<br />

was obliterated, seems in the case <strong>of</strong> rare foliose<br />

and fruticose lichens in many ways extremely<br />

unlikely. This is valid especially for species,<br />

which reproduce with the help <strong>of</strong> relatively heavy<br />

soredia and isidia.<br />

3. Investigations and<br />

Determinations <strong>of</strong> <strong>Lichens</strong><br />

3.1 Introduction<br />

Most foliose and fruticose lichens may be<br />

determined without more than the aid <strong>of</strong> a<br />

handlens, a few without the aid <strong>of</strong> optical<br />

devices. Important are e.g. form and color <strong>of</strong> the<br />

thallus and the fruiting body, occurrence <strong>of</strong> warty<br />

outgrowths (isidia) and “mealy” eruptions<br />

(soralia) and other organs. For the determination<br />

<strong>of</strong> crustose lichens the use <strong>of</strong> a microscope is as a<br />

rule necessary. Indeed the experienced lichen<br />

specialist can easily distinguish almost all lichens<br />

including the crustose lichens by habitat and<br />

ecological characteristics alone, but in<br />

determination one can not commonly by<br />

external characteristics alone, sufficiently<br />

through our grammatical potentiality, be exact<br />

enough about the fine distinctions in coloring and<br />

structures to define them in keys. For this reason<br />

measurable and clearly defined microscopic<br />

characteristics must be applied, e.g. spore form<br />

and size, structure <strong>of</strong> the fruiting body, coloring<br />

<strong>of</strong> the fruiting body parts etc. For the<br />

determination <strong>of</strong> such characteristics sections<br />

through the fruiting body are <strong>of</strong>ten necessary.<br />

The producing <strong>of</strong> sections is not difficult.<br />

They are made much easier, when a binocular or<br />

a dissecting scope is used, under which one can<br />

work with both hands. At least in the larger<br />

fruiting bodies, sections may be prepared without<br />

the aid <strong>of</strong> lenses.<br />

With one hand one holds the lichen fast, with<br />

the other hand one draws an unused razor blade<br />

parallel (vertical) sectioning through the fruiting<br />

body. At best one removes to begin with a<br />

marginal part, perhaps a fourth or a third <strong>of</strong> the<br />

fruiting body and next cuts the thinnest possible<br />

slice, as from a loaf <strong>of</strong> bread, in many lichens the<br />

fruiting body is easily broken loose and then is<br />

more difficult to manipulate. In this case one can<br />

capture the fruiting body with a tweezers or a<br />

preparation needle and glue it to a wooden or<br />

paper support. The section can be accomplished<br />

dry or (above all, in the case <strong>of</strong> hard fruits better)<br />

with objects swollen with water. It can be very<br />

helpful when one holds the object fast with one<br />

finger so that the fingernail is almost at a right<br />

angle and is used as “backdrop” for the razor<br />

blade. The section may be lifted easily from the<br />

object with the (under the circumstances)<br />

moistened edge <strong>of</strong> the razor blade or with the<br />

point <strong>of</strong> a preparation needle. It is then placed in<br />

a small water drop on the slide and covered with<br />

a cover glass. If the section is not thin enough,<br />

one may squash it somewhat. For which one<br />

places the slide with the cover glass beneath<br />

upon a page <strong>of</strong> filterpaper and presses.<br />

A considerable aid in determination is <strong>of</strong>fered<br />

by color reactions <strong>of</strong> the lichen thallus with<br />

designated chemicals. Many lichens contain the<br />

same substances, which with these substances<br />

yield colored reaction products. The most<br />

important reagents are calcium hydroxide<br />

(KOH), calcium or sodium hypochlorite<br />

(Ca(OCl)2 / NaOCl2) and para-Phenyldediamin<br />

(H2NC6H4NH2). The procurement <strong>of</strong> reagents<br />

becomes increasingly more difficult. Chemical<br />

handling comes into question, under<br />

circumstances even for apothecaries and<br />

druggists. Druggists <strong>of</strong> commercial chains<br />

today do not accept individual orders.<br />

3.2 Important Diagnostic Characteristics<br />

<strong>of</strong> <strong>Lichens</strong><br />

3.2.1 Color <strong>of</strong> the Thallus<br />

8

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