Faculty of Science - Mahidol University

274
IMMUOLOCALIZATION OF THE HIGH
POTENTIAL VACCINE CANDIDATE ANTIGENS
IN THE TEGUMENT OF Fasciola gigantica (NO. 703)
W. Khawsuk, S. Sriburee, N. Soonkhlang, C. Wanichanon, V.
Viyanant, P. Sobhon
Department of Anatomy, Faculty of Science, Mahidol University,
Bangkok, Thailand.
Fasciola gigantica is the causative parasite of the fasciolosis
in the cattle and human. A new way that is chosen to control the
infection is vaccine treatment. Most of proteins that used as vaccine
candidate are the excretory-secretory (ES) proteins. The high
potential vaccine candidates that found in the ES antigens are
glutathione Stransferase (GST), fatty acid binding protein (FABP)
and cathepsin L (Cat L). ES is secreted from gut epithelium, excretory
system and tegument of the parasite. The tegumental antigen is very
interesting because it is directly exposed to the host immune
responses. Therefore, tegumental antigen might be easily to attack
by the vaccine treatment. This study has identified the location of
the high potential antigen, GST, FABP and Cat L, in the tegument
of newly excysted juvenile, 4-week juvenile and adult F. gigantica.
The parasites were fixed with 4% paraformaldehyde and 0.5%
glutaraldehyde. Cryosections were stained by immunofluorescence
technique. LR White ultrathin sections were stained by immunogold
labeling technique and used natural infected serum (CIS), laboratory
infected serum (MIS) and polyclonal antibody against GST, FABP
and Cat L as primary antibodies. The results showed that positive
staining of CIS and MIS were found abundantly in the tegument.
CIS was localized mainly in G2 granule of all stage parasites and
some in G0 and G1 granule. MIS was found in G0 and G1 granules,
and at the glycocalyx coating of the tegument. GST and FABP were
fairly found in the matrix of the tegument but not in the granule or
membrane. Cat L was found only on the glycocalyx coating of the
tegument. These results suggested that the high potential antigens
were found in the tegument, which may be released to be ES antigens.
This study may be used as the basic knowledge for vaccine
development against tropical fasciolosis or for the immunodiagnosis.
(Poster presentation at the 22 nd MST Annual Conference. Journal
of Microscopy Society of Thailand 2005, 19(1): 168-169.)
HISTOLOGY AND ULTRASTRUCTURE OF THE
MEHLIS’ GLAND-OOTYPE COMPLEX IN
Fasciola gigantica (NO. 704)
A. Meepool, C. Wanichanon, V. Viyanant, P. Sobhon
Department of Anatomy, Faculty of Science, Mahidol University,
Bangkok, Thailand.
The Mehlis’ gland-ootype complex is located at the midline
of the parasite body between the testis and the uterus. It is an oval
shave organ, which is composed of a group of cells surrounding the
ootype and its accessory ducts. The Mehlis’ gland cells are unicellular
exocrine gland located around the ootype which are classified into
two types: Mehlis’ gland type 1 and 2 cells. Their cell bodies are
located at the periphery of the gland, and they send their cytoplasmic
processes toward the central part of the gland and enter the ootype
wall to open at the ootype lumen where the eggshell is being formed.
The cytoplasmic processes of the Mehlis’ gland cells are running
between the processes of the parenchymal cells, which help to
support them in positions. Furthermore, the parenchymal cells send
their cytoplasmic processed to contact with the basement membrane
of the ootype wall and its accessory ducts to supply nutrients to
their epithelial cells. In addition to the Mehlis’ gland cells, there are
two types of the nerve cells, type I and II, and muscle cells in the
central part of the gland. The accessory ducts are including the
vitelline reservoir, the median vitelline duct, the oviduct, the Laurer’s
canal and the proximal part of the uterus. The median vitelline duct
extends from the vitelline reservoir to the central part of the Mehlis’
gland and merges with the oviduct to become the ootype, and the
ootypecontinue into the proximal part of the uterus.
(Poster presentation at the 22 nd MST Annual Conference. Journal
of Microscopy Society of Thailand 2005, 19(1): 170-171.)
EFFECTS OF PSEUDOEPHEDRIEN ADMINIS-
TRATION ON GLUTAMATE/N-METHYL-D-
ASPARTATE RECEPTOR IMMNOREACTIVITY
IN RAT HIPPOCAMPUS (NO. 705)
S. Nudmamud-Thanoi, Thanoi S. and Sobhon P.
Faculty of Science
Department of Anatomy, Faculty of Science, Mahidol University,
Bangkok, Thailand.
Objectives: This study is aimed to investigate the effects
of acute and chronic pseudoephedrine administration on glutamate/
N-methyl-D-aspartate (NMDA) receptors in hippocampus which is
the area involved in learning and memory.
Materials and Methods: Sprague Dawley male rats (250-
280 g) were divided into 3 groups as acute, chronic, and control
groups with 10 animals each. To examine as acute effect of
pseudoephedrine, animals were administered intragastically at the
dose of 120 mg/kg. The chronic effect was examined by treated
pseudoephedrine intragastically at the dose of 80 mg/kg, once daily
for 15 days. The animals in control group were administered
intragastically with vehicle. We employed immunohistochemistry
to determine the alteration of glutamate/M-methyl-D-aspartate
receptors subunit 1 (NMDAR1) immunoreactivity in rat
hippocampus following actue and chronic pseudoephedrine
administration.
Results : Immunohistochemistry demonstrated MNDAR1
immunoreactive cells in all principal neuronal populations of the
hippocampus. Immunoreactivity was accordingly limited to neurons,
was strong in pyramidal cells of cornu ammonis fielsd 1-3 (CA1-3),
and was especially strong in granule cells of dentate gyrus. MNDAR1
immunodensity in three experimental groups were compared by
analysis of variance (ANOVA) with Dunnett post hoc tests.
NMDAR1 immunodensity was significantly increased above control
in dentate gyrus in acute (p