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http://legacy.library.ucsf.edu/tid/hvk78d00/pdf

http://legacy.library.ucsf.edu/tid/hvk78d00/pdf

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'1 / /%/Qe _ y ?-<br />

`}^ '<br />

I.^+K^il"^Cw t:1 NIOI'H1^51CAy/.CIA<br />

1 ^ ItnA 663]{<br />

.<br />

•Pi:ti^^gR9`1t^T'XS.'}T:]^i:I^3^F4T1iCb1``R'.4'TL•it=1K:rZ^}.lit}LE,_<br />

'=^l?t'IS^1`^T3SY;SUEt.7[]}301Y'.CL'E::SF^' .^::_. ' -<br />

50269 9737<br />

C-LEG T r<br />

nrprrl.+vx! /11++vLri+iir/.y,T4!'.i+rrsilYnf7raw.1lydir^l$[imlatSaa.fnlanio,SanArlonio,<br />

Trie1 7d-9 (U.S.A.)<br />

(I2tteF'rd )auoary r3^h. 97r)<br />

Sr-/tr]Ar:l-<br />

Sereral enzrmaticalhY active walrninwluLle ril.onuclease T, (rilwnuclcate<br />

(vaninenudeo<strong>tid</strong>e-z'-Iranslrrasc (crclidnr), LC 2.7.7.76) derivatives were prepared.<br />

One of thr>c, SepLarme Tr, ai:id: rras ptepmed by ehemicalle coup.ing ritnanucieaee<br />

T, to Sepharose, wzs funLer diararterir.ed. The enzvme derivative was stable and<br />

had no detectable residual snluLle enzrmatit ac<strong>tid</strong>tc. Substrate specificitp of the<br />

enz)-me derivacice remained unaltered. Ainetic values were similar to the freq native<br />

er1Z\T1C. , ,<br />

Cl / () 0 il .. 2 1 ; 9<br />

<strong>http</strong>://<strong>legacy</strong>.<strong>library</strong>.<strong>ucsf</strong>.<strong>edu</strong>/<strong>tid</strong>/<strong>hvk78d00</strong>/<strong>pdf</strong><br />

435

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