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Plate No Rle Between pages Callused cotyledonary stage immature zygotic embryo of C. cajan cv ICPL 87 cultured on MS medium supplemented with 54 pM NAA showing globular and torpedo stage somatic embryos. 47-48 Abnormal somaiic embryo with fused cotyledons on 54 pM NAA. Precocious germination of the abnormal somatic embryo on hormone-free medium. Callus squash of mature zygotic embryos of C, cajan cv ICPL 87 cultured on MS medium plus 54 VM NAA exhibiting actively dividing embryogenic cells. 47-48 7-8 celled proembryoid in callus of malure zygotic embryo cultured on 54 pM NAA. 8-10 celled proembryoid on same medium. 16-20 celled proembryoid on same medium. Mature Imbibed cotyledon d C. cajan cv ICPL 87 cultured on MS medium plus 17 pM BA and 3 pM p-CPA producing globular sornatlc embryos. 48-49 Histological section of mature cotyledon (horn 3A) with globular sornatlc embryos having distinct epidermal layer and Independent vosculature.
4 A Mature cotyledon of C. cajan cv ICPL 87 cultured on MS medium supplemented 21.18 pM BA and 5.37 pM NAA producing green nodular outgrowths. 48-49 B Mature cotyledon of C. cajan cv ICPL 87 cultured on 2.22 pM BA and 0.54 pM NAA showing proliferation of shoot buds from the green nodular outgrowths seen In 4A. 5 A Seven day seedling cotyledon of C, cajan cv ICPL87 cultured on 12 medium supplemented with 8.9 pM producing adventitlous shoots from nodular protuberances at proximal region after 3 wk in culture. 49-50 B Cotyledon cultures (from 5A) transferred to 0.4 pM BA, 1% actlvded charcoal and 2% sucrose showing elongating shook at 8 to 9 weeks in culture. C An elongded shoot rooted alter 2 wk on 12 hormone-free medium. D Acclimatization of a rooted plantlet. 6 Morphological variations observed in R1 generation of pigeonpea (Cajanus cajan (1.) Millsp.) in the greenhouse. 70-71 A-C Variation in number of wing and standard petals. F Flower with a petaloid sepal. E An abnormal flower giving rise to two addiilonal flowers. 0 Stamens prof~ding out of a flower bud. G Petaloid stamens. H A dissected flower with 2 gynoecia. I lhe two gynoecia produclng twin pods. J The three gynoecia producing hlple pods. K Flower with three gynoecla. L A norm1 regenerded plant. 7 Phenotypic variation In the field grown R2 generation d plgoonpea (Cajanus cajan (1.) Mlllsp.). 70-71 A An Indeterminate inflorescence. I) A semi-determinate Inflorescence.
Page 1 and 2: Application of Somaclonal Variation
Page 3 and 4: DECLARATION The research work embod
Page 5 and 6: Depaltment of Botany, 0.U) for beln
Page 7 and 8: US OF TABLES Between Pages Inorgani
Page 9: 21 Source of variation for the yiel
Page 13 and 14: No Tie i b UST OF FIGURES 1 lnlluen
Page 15 and 16: evollian of the thearetic01 asprt.
Page 17 and 18: used for identification of randomly
Page 19 and 20: on culture induced vs mutagen induc
Page 21 and 22: egions could resun in various anora
Page 23 and 24: In their efforts to select for Fusa
Page 25 and 26: Herftable resislance to non-specUlc
Page 27 and 28: profdn. They observed 23 ol91 mutog
Page 29 and 30: following plant rogoneration In 9 d
Page 31 and 32: per spike, kernel weight, yidd, tot
Page 33 and 34: ange from 11.5 to 39.5% and a tende
Page 35 and 36: nultlplo shoots, dwarts, abnormal l
Page 37 and 38: waxiness. glume and grain colour, g
Page 39 and 40: 2.2 MOLECULAR STUDIES OF SOMACLONAL
Page 41 and 42: one regenerant which was hetetozygo
Page 43 and 44: when hybddizatlon pattern of regene
Page 45 and 46: 2.3 TISSUE CUlWRE AND REGENERATION
Page 47 and 48: lomuldlon has Increased concenhatlo
Page 49 and 50: A compehenslve llst of whole plant
Page 51 and 52: TYPO Of Spocler regoneratlon Explan
Page 53 and 54: cotyledons. Rwna et al. (1992) rege
Page 55 and 56: 3. TISSUE CULTURE AND REGENERNION I
Page 57 and 58: Immature cotyledon explants obfdned
Page 59 and 60: cultured on MS semi-sdid medium sup
Page 61 and 62:
In vltro grown seedllngs were used
Page 63 and 64:
Three weeks after culture all the g
Page 65:
PLATE 1 1 A. Callused cotyledonary
Page 68 and 69:
PLATE 2
Page 70 and 71:
3A. Mature imbibed cotyledon ot C.
Page 72 and 73:
4A. lvlature colyiedo~? of CJ cujar
Page 74 and 75:
Effect of different hormonal comb~n
Page 76 and 77:
E 9 Cuiture Period (weegsj NO shoot
Page 78 and 79:
PLATE 5
Page 80:
IncreoJng the doses of rlrradlation
Page 84 and 85:
3.3 DISCUSSION Auxins In the presen
Page 86 and 87:
axes when cultured on MS medium sup
Page 88 and 89:
In the present investigation the In
Page 90 and 91:
oMaln cotyledonary node tissue of s
Page 92 and 93:
Bama and Wakhiu (1994) oberved high
Page 94 and 95:
species producing pdyphenols In cul
Page 96 and 97:
4 ASSESSMENT OF SOMACLONAL VARIATIO
Page 98 and 99:
a RI Generafton: The R1 plants grow
Page 100 and 101:
Seed number per pod: Total number o
Page 102 and 103:
4.2 RESULTS 4.21 SOMACLONAL VARIATI
Page 105:
Phenotyprc variation in the field g
Page 108 and 109:
Frequency of phenotvplc varlation s
Page 110 and 111:
Table 8 Genotypic varlation in qual
Page 112 and 113:
Figure:i Ward's minimum variance de
Page 114:
Cluster means for the yleld paramet
Page 119 and 120:
Analysis of varlance for various yi
Page 121 and 122:
4.23 GAMMA-lRUD1ATED VS UN-IWADIAlE
Page 123 and 124:
Cluster means lor the yield paramet
Page 126 and 127:
Cluster means for the yield paramet
Page 128 and 129:
lowed ped incidence groups. Althoug
Page 130 and 131:
Analysis of varlance (GLM) for varl
Page 132 and 133:
with hawest index having the least
Page 134 and 135:
Table 22 Source of variation lor th
Page 136 and 137:
Phenolyplc cndgenofypic vorfollon f
Page 138 and 139:
PLATE 8
Page 140 and 141:
Table 25 Comparlson of Hellcoverpa
Page 142 and 143:
Fimnotyplc varkrfion torquditailve
Page 144 and 145:
mutations from recessive to dominan
Page 146 and 147:
The plants regenerated hom gamma in
Page 148 and 149:
ecovery ot such agronomically usefu
Page 150 and 151:
The dilferenltal exprdon d tomaclon
Page 152 and 153:
Ealy maturlty wor obtwed In malze b
Page 154 and 155:
MOLECULAR ANALYSIS OF SOMACLONAL PR
Page 156 and 157:
5.1.3 DNA AMPUFICATION AND PCR COND
Page 158:
Fruciionation of PCR prod~rcfs of t
Page 161 and 162:
5.3 DISCUSSION Among the varlants s
Page 163 and 164:
GENERAL DISCUSSION Chapter 6 The pr
Page 165 and 166:
thw to rnetaboliw BA quite efllclml
Page 167 and 168:
which adsoltx hydroxymelhyl-fuhrral
Page 169 and 170:
this study there has been no r.pott
Page 171 and 172:
fu- develop somaclonal lines usable
Page 173 and 174:
Due to the lack d sufficient geneti
Page 175 and 176:
of DNA sequences have been obwwed I
Page 177 and 178:
SUMMARY AND CONCLUSIONS Chapter 7 T
Page 179 and 180:
tho pufative somaclones with agrono
Page 181 and 182:
or large pod bearing length per bra
Page 183 and 184:
Preliminary analysis of the putativ
Page 185 and 186:
Bootliein, DA and McDanW, R.G. 1987
Page 187 and 188:
Brown, P.T.H and Lkz, H. 1986. Meth
Page 189 and 190:
Ween, LS., SMhman, D.D and Rlnw, H.
Page 191 and 192:
Franklln C.I., Revw, N., Gronzales,
Page 193 and 194:
Groacs, R.W., and Binghcm, E.T. (19
Page 195 and 196:
Johnson, L.B., SMenllle, D.1, Schla
Page 197 and 198:
KWy, W and H.J. Jacabson. 1990. Som
Page 199 and 200:
Maddock, S.E. RWott, R., Pomr, S.,
Page 201 and 202:
Mdns, F., Jr. 1983. Heritable varia
Page 203 and 204:
Niizeki, M., ishikawa, R and Sdto,
Page 205 and 206:
Powell. W., Hayter, A.M. Wood, W, D
Page 207 and 208:
Sanlos, A.V.P. dm, Outka, D.E., Coc
Page 209 and 210:
Sun, Z., Zhao. C., Zheng, K., Qi, X
Page 211 and 212:
Yu, K., Chdme, 0.R and Pwls, K.P. 1
Page 213 and 214:
The letter 'R' in this study indica
Page 215:
Presentations: PRASANNA LATHA, CH.,
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