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genobp. on tho aMl)h/ d Imrnat~re soybean cotyledons to undergo cuxln dmulated somatic
embryogenesis. Among the 33 lines studled, good regenerallon ability was evident In those
with one or both of the highly regenerable lines ('Manchu' and 'A.K.Horrow') in their pedigree.
The F1 hybrid cotyledons oblalned trom a cross between 'Manchu' and 'Shiro' (a po~ly
regenerating line) showed intermediate regenerative capacity. Barwale el al. (1986) Induced
multiple shools in vltro trom 155 Glycine max and 13 Glycine so/a genotypes of rnaturlty
groups 'OW' to 'VII' when cuHured on 85 medlum supplemented I or 5 VM BA. Genotype and
hormone concentration specific responses were observed wilh number d shook ranging lrom
1 to 12 for different genotypes. All these case studies show regeneration to be genotype
specific.
2.3.1 nSSUE CULNRE MEDU
The requirements d plant tlssues grown in vlro are similar in general to those of Intact plants
growing in nature. But the Isolated tissues and organs lack the capacity to synthesize their
own supply of carbohydrates, most vitamins and plant growth substances for whole plont
regeneration. A major Mort in developing a plan1 tiuue culture medium was made by
Murashige and Skoog (1962) which is the now universally used MS medium. It was infflally
used lo opltmize the growth d tobacco pHh cdius in vltro. While Murashige and Skoog (1962)
determined the mineral nuhient requirements, Unmoor and Skoog (1965) in their LS medium
examlned the organic growth requirernenk and found that only thlamine k important. Later
medlo (BS) doveloped by Camborg et 01. (1968) had subslontialty higher levels of potdum
salts and lower levels of arnmonlum. Chu (1978) developed the N6 medium for cereal anther
culture. The I2 medium of Philllpsmd Collins (1979) contalnsthe major xrltstomloflon similar
to that d MS, but wlM a reduclton In the concontrailon of ammonium (NH.7 and I n c h
phorphab (PO:'), pokrssium (K7, magnesium (Mg17, and calcium (Ca27. The organlc