Download - OAR@ICRISAT

obsefved with mod belng dwarts and late flowering. Two of Me morphologlcal voddnta were
hexaploids and fertile.
Groose and Blngham (1984, 1986) studled for sh& in traits like anthocyanin pigmentation,
mulflfollate leaves, abilii to regenerate and cytoplasmic male sterility as well as vigor,
morphology and ploidy in tetraplold alfalfa regenerated from callus and suspension cultures.
Twenty one percent of 1 16 regenerated plants were considered to be variant for one or more
characten. They also reported a white flowered mutant In tissue cultured tetraplaid alfalfa wlfh
purple flower. The lunclonal allele C2 of a locus required for anthocyanln pigmentation was
in a simplex condition C2c2c2c2 in the donor genotype which mutated to an unstable
recessive ('mutable') ollelec2-m4. Nagarajan and Wolton (1987,1989) repolted the polyploids
to be more sfernmy with long Internodes and 'gigas' characteristic for led size and thickness
of stem. They also reported that the somaclonal population of alfalfa gave dry molter yields
about 29% lower than Me synthetic populalion from which they were derlved. Similar lower
values were also observed for plant height, stem length, number of nodes per stem and
internode length. Johnson et al. (1984) observed m e alfalfa protoclones regenerated from
non-mutagenired rnesophyll protoplorts to be more susceptible to winter damage than heir
parents, They also observed vadous other phenotypes like chiply wrated leaflets, Irregular
leaflet margins, narrow or wide leaflets, shortened internodes, stunting, thickened stem and
self sterility. Protoclones signflcantly superior, equal and inferior (PO.05) in dry weight forage
yields were obtalned with the ldhw two classes ptedomlnating. One RS-K2 protoclone
appeared to exhibit ruktance to Fwariom. Rekch and Binghan (1981) reported variation for
herbage yleld.
In Glyclne rnax (soybean), Banvale and Widholm (1987,1990) observed lethal sectorld alblnas
In Me lnMal regeneranh from immature embryos. The $elfed gmerdon showed twin W ~S,