B. fragilis

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Identification of clinically relevant pathogens (1) Enterobacteriaceae (Conway, et al. J Mol Microbiol Biotechnol 2001) Genetically distinct E. coli strains could be identified and distinguished from several other genera/species of Enterobacteriaceae Non-fermenters (Mellmann et al. J Clin Microbiol 2008 and 2009) - 248 culture collection strains belonging in 37 genera were used to set up the database - MALDI-TOF identified 67 of 78 (85.9%) clinically relevant non-fermenters concordant with the sequencing - Mean log(score) values for 60 blind-coded samples identified by 8 laboratory were evaluated 97.3% of all log(score) values were >2 10
Identification of clinically relevant pathogens (2) Staphylococci (Carbonnelle et al. J Clin Microbiol 2007; Szabados et al. J Med Microbiol 2010) Enterococci (Eigner et al. Clin Lab 2009) Streptococci (Friedrichs et al. J Clin Microbiol 2007) Neisseria spp (Ilina et al. J Mol Diagn 2009) Listeria monocytogenes (Barbuddhe et al. Appl Environ Microbiol 2008) Mycobacteria (Pignone et al. J Clin Microbiol 2006) Etc. 11
Page 1 and 2: “No Reservations” Acceptance an
Page 3 and 4: Only 12 of 23 Bacteroides species a
Page 5 and 6: History 1975: the first use of MS
Page 7 and 8: m/z Technical description of MALDI-
Page 9: Sample preparation Direct transfer
Page 13 and 14: ~5000 clinical isolates; 2009-2010
Page 15 and 16: - By analysis of MS of 25 MRSA isol
Page 17 and 18: Shah et al. CID 35: S58-64 (2002)
Page 19 and 20: Species determination of 277 Bacter
Page 21 and 22: Routine identification of anaerobic
Page 23 and 24: Dendogram of Bacteroides/Parabacter
Page 25 and 26: Use of MALDI-TOF MS to distinguish
Page 27 and 28: Gel/stack view of MSP: zoom 4000 to
Page 29 and 30: B. fragilis strains tested blindly
Page 31 and 32: Dendogram of B. fragilis isolates a
Page 33 and 34: The studies carried out on the iden

B. fragilis

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