B. fragilis

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Comparative identification of 17 Gram-positive anaerobic clinical isolates Biochemical identification Analyte Organism Score MALDI Biotyper Name (best match) Value Finegoldia magna x HU 29644_1 PNU not reliable identification 1.238 Clostridium perfringens x HU66505 PNU Clostridium perfringens 2.473 Clostridium perfringens x HU65634 PNU Clostridium perfringens 2.457 Clostridium perfringens x HU65618 PNU Clostridium perfringens 2.412 Clostridium perfringens x HU64840 PNU Clostridium perfringens 2.439 Clostridium perfringens x HU64432 PNU Clostridium perfringens 2.45 Clostridium difficile x HU59744 PNU Clostridium difficile 2.552 Clostridium sp x HU56448 PNU Clostridium innocuum 2.3 Clostridium perfringens x HU55650 PNU Clostridium perfringens 2.444 Clostridium perfringens x HU53309 PNU Clostridium perfringens 2.434 Clostridium perfringens x HU52508 PNU Clostridium perfringens 2.414 Clostridium clostridioforme x HU52042 PNU not reliable identification 1.406 Clostridium perfringens x HU51221 PNU Clostridium perfringens 2.398 Clostridium tertium x HU46926 PNU Clostridium tertium 2.351 Clostridium innocuum x HU46588 PNU Clostridium innocuum 2.481 Clostridium perfringens x HU39493 PNU Clostridium perfringens 2.475 Clostridium fallax x HU33416 PNU not reliable identification 1.313 16S rRNA F. magna C. innocuum C. hathewayi B. ureolyticus 24
Use of MALDI-TOF MS to distinguish cfiApositive and cfiA-negative B. fragilis strains (typing of B. fragilis) Carbapenem resistance in B. fragilis is due in most cases to the presence of the cfiA gene and its expression (IS elements are needed in the promoter region) This metallo-beta-lactamase confers resistance to all betalactam antibiotics Only B. fragilis strains belonging in Division II harbor the cfiA gene Different studies indicate an increased resistance of B. fragilis to carbapenems and an increased frequency of silent harboring of the gene 25
Page 1 and 2: “No Reservations” Acceptance an
Page 3 and 4: Only 12 of 23 Bacteroides species a
Page 5 and 6: History 1975: the first use of MS
Page 7 and 8: m/z Technical description of MALDI-
Page 9 and 10: Sample preparation Direct transfer
Page 11 and 12: Identification of clinically releva
Page 13 and 14: ~5000 clinical isolates; 2009-2010
Page 15 and 16: - By analysis of MS of 25 MRSA isol
Page 17 and 18: Shah et al. CID 35: S58-64 (2002)
Page 19 and 20: Species determination of 277 Bacter
Page 21 and 22: Routine identification of anaerobic
Page 23: Dendogram of Bacteroides/Parabacter
Page 27 and 28: Gel/stack view of MSP: zoom 4000 to
Page 29 and 30: B. fragilis strains tested blindly
Page 31 and 32: Dendogram of B. fragilis isolates a
Page 33 and 34: The studies carried out on the iden

B. fragilis

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