B. fragilis

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Comparative species determination of selected Bacteroides strains Sample number Species determined by _______________________________________________________________________________________________ Biochemical tests MALDI-TOF MS 16S rDNA sequencing 1 B. ovatus B. fragilis B. fragilis 2 B. uniformis B. fragilis B. fragilis 3 B. ovatus B. thetaiotaomicron B. thetaiotaomicron 4 B. uniformis B. thetaiotaomicron B. thetaiotaomicron 5 B. uniformis B. thetaiotaomicron B. thetaiotaomicron 6 B. ovatus B. thetaiotaomicron B. thetaiotaomicro 7 B. uniformis B. ovatus B. ovatus 8 B. thetaiotaomicron B. vulgatus (log(score) 2.023) Bacteroides sp (new?) 9 B. ovatus B. uniformis B. uniformis 10 B. ovatus B. uniformis B. uniformis 11 B. ovatus B. nordii B. nordii _______________________________________________________________________________________________ 12 B. thetaiotaomicron II*(log(score)>2.5) P. (B.) distasonis 13 P. (B.) distasonis II P. (B.) distasonis 14 P. (B.) distasonis II(log(score)>2.5) P. (B.) distasonis 15 P. (B.) distasonis II(log(score)>2.5) P. (B.) distasonis 16 B. thetaiotaomicron II B. eggerthii 17 B. merdae II B. goldsteinii 18 Bacteroides sp II B. intestinalis *II = Inconclusive identification Nagy et al. CMI 15: 796-802 2009 20
Routine identification of anaerobic bacteria by MALDI-TOF Stingu et al: Oral Microbiology Immunology 2008 84 oral biofilm isolates (including 9 reference strains) were tested MALDI-TOF was superior to biochemical identification P. intermedia/nigrescens (37 strains) could be distinguished by MALDI-TOF MS Veloo et al: CMI 2010 79 clinical isolates representing 19 genera Comparison of Bruker and Simadzu system with 16S rRNA gene sequencing Correct identification at a genus level 61% versus 71% and at a species level 51% versus 61% When species not present in the database were excluded 75% versus 76.7% 21
Page 1 and 2: “No Reservations” Acceptance an
Page 3 and 4: Only 12 of 23 Bacteroides species a
Page 5 and 6: History 1975: the first use of MS
Page 7 and 8: m/z Technical description of MALDI-
Page 9 and 10: Sample preparation Direct transfer
Page 11 and 12: Identification of clinically releva
Page 13 and 14: ~5000 clinical isolates; 2009-2010
Page 15 and 16: - By analysis of MS of 25 MRSA isol
Page 17 and 18: Shah et al. CID 35: S58-64 (2002)
Page 19: Species determination of 277 Bacter
Page 23 and 24: Dendogram of Bacteroides/Parabacter
Page 25 and 26: Use of MALDI-TOF MS to distinguish
Page 27 and 28: Gel/stack view of MSP: zoom 4000 to
Page 29 and 30: B. fragilis strains tested blindly
Page 31 and 32: Dendogram of B. fragilis isolates a
Page 33 and 34: The studies carried out on the iden

B. fragilis

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