B. fragilis

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An anaerobic clincal isolate Identification view: sample MSP stick spectrum (upside) and reference MSP stick spectrum (downside) Gel overview: view of sample MSP MALDI-TOF Biotyper Taxonomy tree view: Based on NCBI taxonomy browser MSP results and log(score) values 8
Sample preparation Direct transfer of a colony of bacteria or yeasts onto the target or Pretreatment: One colony is suspended in 300 μl bidistilled water 900 μl ethanol is added and mixed thoroughly Centrifugation for 2 min – the pellet is dried Re-suspend in up to 50 μl 70% aqueous formic acid and 50 μl acetonitrile Centrifugation for 2 min and 1 μl supernatant is spotted on the target plate and dried at room temperature (in the case of some pathogens special sample preparation is needed) Pretreated and untreated samples are overlaid with 1 μl matrix solution Measurements are usually made on multiple parallels 9
Page 1 and 2: “No Reservations” Acceptance an
Page 3 and 4: Only 12 of 23 Bacteroides species a
Page 5 and 6: History 1975: the first use of MS
Page 7: m/z Technical description of MALDI-
Page 11 and 12: Identification of clinically releva
Page 13 and 14: ~5000 clinical isolates; 2009-2010
Page 15 and 16: - By analysis of MS of 25 MRSA isol
Page 17 and 18: Shah et al. CID 35: S58-64 (2002)
Page 19 and 20: Species determination of 277 Bacter
Page 21 and 22: Routine identification of anaerobic
Page 23 and 24: Dendogram of Bacteroides/Parabacter
Page 25 and 26: Use of MALDI-TOF MS to distinguish
Page 27 and 28: Gel/stack view of MSP: zoom 4000 to
Page 29 and 30: B. fragilis strains tested blindly
Page 31 and 32: Dendogram of B. fragilis isolates a
Page 33 and 34: The studies carried out on the iden

B. fragilis

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