B. fragilis

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Possibilities for species determination in routine laboratories Classical methods using phenotypic characteristics Automation of biochemical tests Use of short-chain fatty acid production (anaerobic bacteria) DNA-based identification (DNA-DNA hybridization) 16S rRNA gene sequencing (whole DNA sequencing ) Protein-based identification (based on small ribosomal proteins) by mass spectrometry 4
History 1975: the first use of MS for bacterial identification (Anhalt et al. Anal Chem 1975) Limited mass range (
Page 1 and 2: “No Reservations” Acceptance an
Page 3: Only 12 of 23 Bacteroides species a
Page 7 and 8: m/z Technical description of MALDI-
Page 9 and 10: Sample preparation Direct transfer
Page 11 and 12: Identification of clinically releva
Page 13 and 14: ~5000 clinical isolates; 2009-2010
Page 15 and 16: - By analysis of MS of 25 MRSA isol
Page 17 and 18: Shah et al. CID 35: S58-64 (2002)
Page 19 and 20: Species determination of 277 Bacter
Page 21 and 22: Routine identification of anaerobic
Page 23 and 24: Dendogram of Bacteroides/Parabacter
Page 25 and 26: Use of MALDI-TOF MS to distinguish
Page 27 and 28: Gel/stack view of MSP: zoom 4000 to
Page 29 and 30: B. fragilis strains tested blindly
Page 31 and 32: Dendogram of B. fragilis isolates a
Page 33 and 34: The studies carried out on the iden

B. fragilis

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