Wolf PDF Cover - Wolf Laboratories
Wolf PDF Cover - Wolf Laboratories
Wolf PDF Cover - Wolf Laboratories
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<strong>Wolf</strong> <strong>Laboratories</strong> Limited<br />
www.wolflabs.co.uk<br />
Tel: 01759 301142 Fax:01759 301143 sales@wolflabs.co.uk<br />
Use the above details to contact us if this literature doesn't answer all your<br />
questions.<br />
Pricing on any accessories shown can be found by keying the part number<br />
into the search box on our website.
Leica CM1950 Configuration Europe<br />
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />
specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />
AgProtect nanosilver coating, disposable blade holder CE.<br />
Instrument features:<br />
Freestanding cryostat with encapsulated, splashproof microtome. Spacious,<br />
stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />
disinfect. Heated, removable sliding window. Stable, selfcontained<br />
cryocabinet on rollers. Collection container for condensing water at front of<br />
instrument. Outer surfaces and controls of instrument coated with AgProtect<br />
nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />
two positions. Easytohandle and stable clamping system for clamping the<br />
specimen discs. 8 ° XY specimen orientation with zero point reference.<br />
Specimen retraction for manual mode of operation, (20 m), can be switched<br />
off.<br />
Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />
Disinfection can be aborted at any time, if work needs to be resumed<br />
immediately. Automatic safety cut-off of disinfection cycle when sliding<br />
window is opened.<br />
Twin compressor cooling system for cryochamber and specimen head.<br />
Cooling system with safety pressure control. Specimen temperature<br />
selectable from -10°C to -50°C in 1 ° steps.<br />
Cryochamber temperature selectable from 0 °C to<br />
• 35 °C, +5K, adjustable in 1K increments atambient temperature of 20<br />
°C.<br />
Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />
shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />
Peltier element freezing stations (temperatures down to -52°C) with manual<br />
defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />
every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />
minutes.<br />
8
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />
elements and specimen head with acoustic warning signal to prevent<br />
unintentional defrosting. Manual defrost cycle for Chamber and quick-freezing<br />
shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />
Low-maintenance microtome with cross roller guides. Reproducible highquality<br />
thin sections via stepper motor specimen feed. Section thickness<br />
selection from outside the cryochamber.<br />
Section thickness selection:<br />
Trimming thickness range: selectable via key combination when instrument is<br />
being switched on.<br />
For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />
For research applications: 1600 m:<br />
selectable in 1 m steps from 110 m<br />
selectable in 2 m steps from 1020 m,<br />
selectable in 5 m steps from 2050 m<br />
selectable in 10 m steps from 50100 m<br />
selectable in 50 m steps from 100600 m.<br />
Sectioning thickness range: 1100 m,<br />
selectable in 0.5 m steps from 15 m<br />
selectable in 1 m steps from 520 m<br />
selectable in 5 m steps from 2060 m<br />
selectable in 10 m steps from 60100 m.<br />
Total vertical specimen stroke: 59 mm<br />
Total horizontal specimen feed: 25 mm<br />
Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />
Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />
Control panel with foil-protected buttons and locking function.<br />
Self-explanatory symbols for all essential functions and displays.<br />
LEDdisplay for cryochamber and specimen head temperature, actual time,<br />
defrost time.<br />
Visual indication of specimen stop positions (front/home).<br />
Blade holder base with segment arc for blade holder CE for high and low<br />
profile disposable blades. Blade holder CE for high and low profile blades with<br />
lateral adjustment, clearance angle adjustment and integrated knife guard<br />
with blade ejection lever.<br />
Manufactured in compliance with cCSAUS and CE standards.<br />
CFCfree.<br />
Technical data:<br />
• Mains power supply: 230 V/50 Hz (European version)<br />
• Admissions: c_CSA_US, CE<br />
• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />
27.55 (with handwheel: 2.87) x 33.46 x 47.84 inches<br />
• Weight: 175 kg / 385.46 lbs<br />
9
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
All specifications related to temperature are valid for a room temperature up to<br />
20 °C and a relative air humidity of 60%.<br />
Operating temperature range: +18 °C to 35 °C<br />
Standard delivery:<br />
• 1 Basic instrument (14 0477 42456)<br />
• 1 Handwheel, assy. manual (14 0477 41346)<br />
• 1 Mains cable EU 230 V/16A (14 0411 32570)<br />
• 5 Specimen discs, 30 mm (14 0477 40044)<br />
• 1 Section waste tray (14 0477 40062)<br />
• 1 Retaining device for quick-freezing shelf (14 0477 40080)<br />
• 1 <strong>Cover</strong> for quickfreezing shelf (14 0477 43763)<br />
• 1 Tool set (14 0436 43463):<br />
• 1 Brush, fine (14 0183 28642)<br />
• 1 Leica brush with magnet (14 0183 40426)<br />
• 1 Allen key with handle, size 5 (14 0194 04760)<br />
• 1 Allen key, size 2.5 (14 0222 04137)<br />
• 1 Allen key, size 3.0 (14 0222 04138)<br />
• 1 Allen key, size 4.0 (14 0222 04139)<br />
• 1 Allen key, size 5.0 (14 0222 04140)<br />
• 1 Allen key, size 6.0 (14 0222 04141)<br />
• 1 Allen key, size 1.5 (14 0222 10050)<br />
• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />
• 1 Single-head wrench, size 16 (14 0330 18595)<br />
• 1 Singlehead wrench, size 13 (14 0330 33149)1 Bottle of cryostat oil,<br />
type 407, 50 ml (14 0336 06098) 1 Tissue freezing medium for<br />
cryosectioning, 125 ml (14 0201 08926) 1 Pair of safety gloves, size<br />
"M" for cryocsectioning (14 0340 29011) 1 Blade holder base with<br />
segment arc (14 0477 40351) 1 Disposable blade holder (14 0477<br />
43005) consisting of:<br />
• insert for lowprofile blades (14 0477 40386)<br />
• finger rest for brush technique (14 0477 40389)<br />
• antiroll plate, 70 mm wide, with 50 m spacer and with glare-minimizing<br />
glass insert (14 0477 42491)<br />
• antiroll plate 70 mm wide, with 100 m spacer and glare-minimizing<br />
glass insert (14 0477 42492) 1 EC Declaration of Conformity (14 0477<br />
80011) 1 Instructions for use Leica CM1950 (14 0477 80001)<br />
No : 1491950C2EU<br />
10
Leica CM1950 Configuration Europe<br />
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />
specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />
AgProtect nanosilver coating, motor for motorized sectioning, disposable<br />
blade holder CE<br />
Instrument features:<br />
Freestanding cryostat with encapsulated, splash-proof microtome. Spacious,<br />
stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />
disinfect. Heated, removable sliding window. Stable, self-contained<br />
cryocabinet on rollers. Collection container for condensing water at front of<br />
instrument. Outer surfaces and controls of instrument coated with AgProtect<br />
nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />
two positions. Easytohandle and stable clamping system for clamping the<br />
specimen discs. 8° XY specimen orientation with zero point reference.<br />
Specimen retraction for manual mode of operation, (20 m), can be switched<br />
off. Motorized modes of operation equipped with selfadjusting retraction<br />
system, retraction value depending on sectioning speed.<br />
Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />
Disinfection can be aborted at any time, if work needs to be resumed<br />
immediately. Automatic safety cutoff of disinfection cycle when sliding window<br />
is opened.<br />
Motorized sectioning, single and continuous stroke. Motorized sectioning<br />
operated via control panel or optional foot switch. When motorized sectioning<br />
is stopped via the control panel, the handwheel is automatically locked in the<br />
bottom position. Safety handwheel can be centered during motorized<br />
sectioning. 2 Sectioning speed ranges selectable: 050 strokes per minute or<br />
085 strokes per minute. Vmax button for fast trimming: 85 to 90 strokes per<br />
minute.<br />
Twin compressor cooling system for cryochamber and specimen head.<br />
Cooling system with safety pressure control. Specimen temperature<br />
selectable from -10°C to -50°C in 1 ° steps.<br />
Cryochamber temperature selectable from 0 °C to<br />
11
WOLF LABS<br />
Think sustainable<br />
• 35 °C, +5K, adjustable in 1K increments at ambient temperature of<br />
20°C.<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />
shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />
Peltier element freezing stations (temperatures down to -52°C) with manual<br />
defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />
every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />
minutes.<br />
Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />
elements and specimen head with acoustic warning signal to prevent<br />
unintentional defrosting. Manual defrost cycle for Chamber and quickfreezing<br />
shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />
Low-maintenance microtome with cross roller guides. Reproducible high<br />
quality thin sections via stepper motor specimen feed. Section thickness<br />
selection from outside the cryochamber.<br />
Section thickness selection:<br />
Trimming thickness range: selectable via key combination when instrument is<br />
being switched on.<br />
For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />
For research applications: 1600 m:<br />
selectable in 1 m steps from 110 m<br />
selectable in 2 m steps from 1020 m,<br />
selectable in 5 m steps from 2050 m<br />
selectable in 10 m steps from 50100 m<br />
selectable in 50 m steps from 100600 m.<br />
Sectioning thickness range: 1100 m,<br />
selectable in 0.5 m steps from 15 m<br />
selectable in 1 m steps from 520 m<br />
selectable in 5 m steps from 2060 m<br />
selectable in 10 m steps from 60100 m.<br />
Total vertical specimen stroke: 59 mm<br />
Total horizontal specimen feed: 25 mm<br />
Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />
Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />
Control panel with foil-protected buttons and locking function.<br />
Self-explanatory symbols for all essential functions and displays.<br />
LEDdisplay for cryochamber and specimen head temperature, actual time,<br />
defrost time. Visual indication of specimen stop positions (front/home).<br />
Blade holder base with segment arc for blade holder Ce for high and low<br />
profile disposable blades.<br />
Blade holder CE for high and low profile blades with lateral adjustment,<br />
clearance angle adjustment and integrated knife guard with blade ejection<br />
lever. Manufactured in compliance with cCSAUS and CE standards.<br />
CFCfree.<br />
12
WOLF LABS<br />
Think sustainable<br />
Technical data:<br />
• Mains power supply: 230 V/50 Hz (European version)<br />
• Admissions: c_CSA_US, CE<br />
• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />
27.55 (with handwheel: 32.87) x 33.46 x 47.84 inches<br />
• Weight: 185 kg / 407.49 lbs<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
All specifications related to temperature are valid for a room temperature up to<br />
20 °C and a relative air humidity of 60%.<br />
Operating temperature range: +18 °C to 35 °C<br />
Standard delivery:<br />
1 Basic instrument (14 0477 42458)<br />
1 Handwheel, assy. motorized (14 0477 41347)<br />
1 Mains cable EU 230 V/16A (14 0411 32570)<br />
5 Specimen discs, 30 mm (14 0477 40044)<br />
1 Section waste tray (14 0477 40062)<br />
1 Retaining device for quick-freezing shelf (14 0477 40080)<br />
1 <strong>Cover</strong> for quick-freezing shelf (14 0477 43763)<br />
1 Tool set (14 0436 43463):<br />
• 1 Brush, fine (14 0183 28642)<br />
• 1 Leica brush with magnet (14 0183 40426)<br />
• 1 Allen key with handle, size 5 (14 0194 04760)<br />
• 1 Allen key, size 2.5 (14 0222 04137)<br />
• 1 Allen key, size 3.0 (14 0222 04138)<br />
• 1 Allen key, size 4.0 (14 0222 04139)<br />
• 1 Allen key, size 5.0 (14 0222 04140)<br />
• 1 Allen key, size 6.0 (14 0222 04141)<br />
• 1 Allen key, size 1.5 (14 0222 10050)<br />
• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />
• 1 Single-head wrench, size 16 (14 0330 18595)<br />
• 1 Single-head wrench, size 13 (14 0330 33149)1 Foot switch dummy<br />
(14 0443 30420) 1 Bottle of cryostat oil, type 407, 50 ml (14 0336<br />
06098) 1 Tissue freezing medium for cryosectioning, 125 ml (14 0201<br />
08926) 1 Pair of safety gloves, size "M" for cryocsectioning (14 0340<br />
29011) 1 Blade holder base with segment arc (14 0477 40351) 1<br />
Disposable blade holder (14 0477 43005) consisting of:<br />
• insert for low profile blades (14 0477 40386)<br />
• finger rest for brush technique (14 0477 40389)<br />
• antiroll plate 70 mm wide, with 50 m spacer,with glare-minimizing<br />
glass insert (14 0477 42491)<br />
• antiroll plate 70 mm wide, with 100 m spacer,with glare-minimizing<br />
glass insert (14 0477 42492)<br />
• 1 EC Declaration of Conformity (14 0477 80011) 1 Instructions for use<br />
Leica<br />
• CM1950 (14 0477 80001)<br />
No : 1491950C4EU<br />
13
Leica CM1950 Configuration Europe<br />
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />
specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />
AgProtect nanosilver coating, vacuum system and motor for motorized<br />
sectioning. with blade holder CE<br />
Instrument features:<br />
Freestanding cryostat with encapsulated, splash-proof microtome. Spacious,<br />
stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />
disinfect. Heated, removable sliding window. Stable, selfcontained<br />
cryocabinet on rollers. Collection container for condensing water at front of<br />
instrument. Outer surfaces and controls of instrument coated with AgProtect<br />
nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />
two positions. Easy-to-handle and stable clamping system for clamping the<br />
specimen discs. 8 ° XY specimen orientation with zero point reference.<br />
Specimen retraction for manual mode of operation, (20 m), can be switched<br />
off. Motorized modes of operation equipped with self-adjusting retraction<br />
system, retraction value depending on sectioning speed.<br />
Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />
Disinfection can be aborted at any time, if work needs to be resumed<br />
immediately. Automatic safety cutoff of disinfection cycle when sliding window<br />
is opened.<br />
Vacuum system for stretching the sections and for removal of section debris,<br />
with saving integrated filter system and bacteria filter on the outside of the<br />
instrument.<br />
Motorized sectioning, single and continuous stroke. Motorized sectioning<br />
operated via control panel or optional foot switch. When motorized sectioning<br />
is stopped via the control panel, the handwheel is automatically locked in the<br />
bottom position. Safety handwheel can be centered during motorized<br />
sectioning. 2 Sectioning speed ranges selectable: 050 strokes per minute or<br />
085 strokes per minute. Vmax button for fast trimming: 85 to 90 strokes per<br />
minute.<br />
14
WOLF LABS<br />
Think sustainable<br />
Twin compressor cooling system for cryochamber and specimen head.<br />
Cooling system with safety pressure control. Specimen temperature<br />
selectable from -10°C to -50°C in 1 ° steps. Cryochamber temperature<br />
selectable from 0°C to<br />
• 35 °C,+5K, adjustable in 1K increments at ambient temperature of 20<br />
°C.<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />
shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />
Peltier element freezing stations (temperatures down to -52°C) with manual<br />
defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />
every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />
minutes.<br />
Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />
elements and specimen head with acoustic warning signal to prevent<br />
unintentional defrosting. Manual defrost cycle for Chamber and quick-freezing<br />
shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />
Low-maintenance microtome with cross roller guides. Reproducible high<br />
quality thin sections via stepper motor specimen feed. Section thickness<br />
selection from outside the cryochamber.<br />
Section thickness selection:<br />
Trimming thickness range: selectable via key combination when instrument is<br />
being switched on.<br />
For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />
For research applications: 1600 m:<br />
selectable in 1 m steps from 110 m<br />
selectable in 2 m steps from 1020 m,<br />
selectable in 5 m steps from 2050 m<br />
selectable in 10 m steps from 50100 m<br />
selectable in 50 m steps from 100600 m.<br />
Sectioning thickness range: 1100 m,<br />
selectable in 0.5 m steps from 15 m<br />
selectable in 1 m steps from 520 m<br />
selectable in 5 m steps from 2060 m<br />
selectable in 10 m steps from 60100 m.<br />
Total vertical specimen stroke: 59 mm<br />
Total horizontal specimen feed: 25 mm<br />
Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />
Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />
Control panel with foil-protected buttons and locking function.<br />
Self-explanatory symbols for all essential functions and displays.<br />
LEDdisplay for cryochamber and specimen head temperature, actual time,<br />
defrost time.<br />
Visual indication of specimen stop positions (front/home).<br />
15
WOLF LABS<br />
Think sustainable<br />
Blade holder base with segment arc for blade holder CE for high and low<br />
profile disposable blades.<br />
Blade holder CE for high and low profile blades with lateral adjustment,<br />
clearance angle adjustment and integrated knife guard with blade ejection<br />
lever.<br />
Manufactured in compliance with cCSAUS and CE standards.<br />
CFCfree.<br />
Technical data:<br />
• Mains power supply: 230 V/50 Hz (European version)<br />
• Admissions: c_CSA_US, CE<br />
• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />
27.55 (with handwheel: 32.87) x 33.46 x 47.84 inches<br />
• Weight: 193 kg / 425.50 lbs<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
All specifications related to temperature are valid for a room temperature up to<br />
20 °C and a relative air humidity of 60%.<br />
Operating temperature range: +18 °C to 35 °C<br />
Standard delivery:<br />
1 Basic instrument (14 0477 42459)<br />
1 Handwheel, assy. motorized (14 0477 41347)<br />
1 Mains cable EU 230 V/16A (14 0411 32570)<br />
1 Extraction accessory set (14 0477 43300):<br />
• 1 Hose adapter 1 (14 0477 40293)<br />
• 1 Hose adapter 2 (0477 40294)<br />
• 1 Extraction nozzle (14 0477 40295)<br />
• 1 Silicon hose (14 0477 43302)<br />
• 1 Silicon stopper (14 0477 43304)<br />
• 1 Cryochamber extraction nozzle (14 0477 43779)<br />
• 1 Set of filters (5 pcs) (14 0477 43792)5 Specimen discs, 30mm (14<br />
0477 40044) 1 Section waste tray (14 0477 40062) 1 Retaining device<br />
for quick-freezing shelf (14 0477 40080) 1 <strong>Cover</strong> for quick-freezing<br />
shelf (14 0477 43763) 1 Tool set (140436 43463):<br />
• 1 Brush, fine (14 0183 28642)<br />
• 1 Leica brush with magnet (14 0183 40426)<br />
• 1 Allen key with handle, size 5 (14 0194 04760)<br />
• 1 Allen key, size 2.5 (14 0222 04137)<br />
• 1 Allen key, size 3.0 (14 0222 04138)<br />
• 1 Allen key, size 4.0 (14 0222 04139)<br />
• 1 Allen key, size 5.0 (14 0222 04140)<br />
• 1 Allen key, size 6.0 (14 0222 04141)<br />
• 1 Allen key, size 1.5 (14 0222 10050)<br />
• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />
• 1 Single-head wrench, size 16 (14 0330 18595)<br />
• 1 Single-head wrench, size 13 (14 0330 33149)1 Foot switch dummy<br />
(14 0443 30420) 1 Bactericidal filter 350/5865 (140477 40296) 1 Bottle<br />
of cryostat oil, type 407, 50 ml (14 0336 06098) 1 Tissue freezing<br />
16
WOLF LABS<br />
Think sustainable<br />
Tel: 01759 301142<br />
Fax: 01759 301143<br />
E-mail: sales@wolflabs.co.uk<br />
medium for cryosectioning, 125 ml (14 0201 08926) 1 Pair of safety<br />
gloves, size "M" for cryocsectioning (14 0340 29011) 1 Blade holder<br />
base with segment arc (14 0477 40351) 1 Disposable blade holder (14<br />
0477 43005) consisting of:<br />
• insert for low profile blades (14 0477 40386)<br />
• finger rest for brush technique (14 0477 40389)<br />
• antiroll plate, 70 mm wide, with 50 m spacerand with glare-minimizing<br />
glass insert (14 0477 42491)<br />
• antiroll plate 70 mm wide, with 100 m spacerand glare-minimizing<br />
glass insert (14 0477 42492) 1 EC Declaration of Conformity (14 0477<br />
80011) 1 Instructions for use Leica CM1950 (14 0477 80001)<br />
No : 1491950C5EU<br />
17
Leica CM1950<br />
THE Disinfecting Clinical Cryostats
Leica CM1950 Platform<br />
THE cryostats for high quality sectioning, user safety and efficient workflow<br />
The Leica CM1950 platform is the latest innovation in a long line of distinguished<br />
cryostats manufactured by Leica Microsystems. Leica designed and developed the<br />
new family of cryostats based upon users’ feedback and their requirements for<br />
high quality sections, user safety, and efficient laboratory workflow.<br />
High Quality Sectioning<br />
2<br />
High precision microtome with stepper motor object feed<br />
Motorized sectioning (optional)<br />
New stable specimen clamp system<br />
New flat specimen discs for high specimen stability<br />
New rapid-freezing shelf design<br />
New effective object cooling system (optional)<br />
User Safety<br />
UVC disinfection<br />
AgProtect antimicrobial nanosilver surface coating<br />
New disposable blade holder CE<br />
Debris-free, frost-free enclosed microtome assembly<br />
Leica’s Section Waste Removal System (optional)<br />
Spacious cabinet with rounded edges inside and out<br />
Efficient Workflow<br />
UVC disinfection at any time and temperature<br />
Organized work space inside and out<br />
Frost-free, encapsulated microtome assembly<br />
Leica’s Section Waste Removal System with easily-accessible,<br />
concealed filter system (optional)<br />
Easily recognizable control symbols<br />
Visible operating parameters at all times<br />
For an extra measure of safety, the inside of<br />
the instrument features an ozone-free UVC<br />
disinfection system that is certified to be<br />
effective against most bacteria, virus, and fungi,<br />
even at cold temperatures.<br />
Increased safety for the user and others in the<br />
immediate work area: Leica’s AgProtect<br />
antimicrobial nanosilver surface coating<br />
deters the propagation of infectious agents on<br />
the outside of the instrument.<br />
Protect<br />
The brigh<br />
cabinet<br />
allow eff<br />
ple spec<br />
Learn quickly and work confidently<br />
using the externally located,<br />
self-explanatory controls<br />
with easily recognizable symbols.<br />
To protect the laboratory environment from<br />
contamination, a multi-filter system (including<br />
HEPA filter) removes particulates and cleans<br />
the air exhausted from the optional Section<br />
Waste Removal System<br />
Liquid condensa<br />
collected in an ea<br />
ble waste contain
tly lit, ergonomic cryos<br />
spacious enough to<br />
cient handling of multimens.<br />
te is safely<br />
sily accessier.<br />
Store tools, glass slides, staining<br />
containers, etc. in a<br />
recessed area on top of the<br />
cryostat to have these items<br />
immediately at hand.<br />
For extra ease of use and consistent<br />
sectioning of all specimen<br />
types, an optional sectioning<br />
motor drive is available.<br />
Leica Design by Werner Hölbl<br />
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4<br />
NEW: CryoZone blade holder and anti-roll guide cooling<br />
Maintaining the ideal object temperature for specific specimens<br />
Great stability of the microtome and blade holder system<br />
Extreme accuracy of the stepper motor object feed<br />
Consistent, motor-driven cutting stroke (optional)<br />
Vacuum-assisted sectioning aid (optional)<br />
High precision microtome with exact stepper motor object feed.<br />
THE cryostats<br />
for high quality sectioning<br />
Obtain high quality sections through:<br />
CryoZone – the cool way to improve section quality<br />
The CryoZone system controls a zone of uniformly cool air in the critical areas of the cryochamber. This<br />
cool air circulates around the specimen, knife and anti-roll guide to create the optimal conditions for consistent,<br />
high-quality sectioning.
Efficient specimen loading is achieved via single lever<br />
operation that provides maximum clamping force independent<br />
of individual user strength.Direct contact cooling<br />
through the (optional) actively cooled object head facilitates<br />
rapid changes of specimen temperatures to provide<br />
the best conditions for sectioning various specimen types.<br />
High specimen stability is achieved by adherence to deeply<br />
grooved specimen discs. Specimens freeze quickly<br />
because the pre-cooled discs feature a large back surface<br />
that fully contacts the freezing shelf with integrated Peltier<br />
element.<br />
Superior sectioning of routine, hard, and/or large samples is<br />
achieved using a choice of knife/blade holders as well as the<br />
new dove tail mounting system that provides increased stability<br />
and extended working distance. The new CE holder for<br />
high and low profile disposable blades evenly distributes<br />
clamping pressure across the entire length of the blade,<br />
which provides vibration-free sectioning.<br />
The new CN and CNZ knife holders are available for steel<br />
or tungsten carbide knives (optional accessories).<br />
The optional vacuum sectioning aid achieves time-saving<br />
section preparation and reduces section curling.<br />
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Histology laboratorires continue to express major concerns<br />
about biohazard exposure and non-ergonomic work practices.<br />
As a result of their feedback, the Leica CM1950 family of<br />
cryostats is designed to provide maximum protection from these<br />
risks at all stages of the sectioning process.<br />
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Certified UVC disinfection of exposed surfaces and air<br />
within the cryochamber at cold temperatures<br />
AgProtect antimicrobial nanosilver coating on the<br />
outside surfaces of the cryostat<br />
Optional Section Waste Removal System with a series of<br />
three filters<br />
Ergonomic exterior cabinet design with rounded edges<br />
and comfortable working height<br />
Optional ergonomic chair and foot rest<br />
THE cryostats<br />
for the highest user safety<br />
Leica’s built-in UVC disinfection system provides<br />
certified protection from infectious material and<br />
minimizes the risk of contamination in the cryochamber.<br />
Scientific studies prove that surface<br />
disinfection from bacteria, viruses, and fungi can<br />
be achieved with UVC light. Certification proving the effectiveness<br />
of UVC disinfection against a range of microorganisms can be<br />
downloaded from www.leica-microsystems.com/cm1950_safety,<br />
which includes detailed information on the test procedure and the<br />
efficacy of UVC disinfection.<br />
To efficiently reduce the propagation of infectious agents on<br />
the outside surfaces of the cryostat, the Leica CM1950 family<br />
features an antimicrobial nanosilver surface coating, called<br />
AgProtect. Silver (Ag) ions are well documented<br />
for their ability to reduce bacterial growth.<br />
Protect<br />
Section waste is easily removed by using Leica’s optional<br />
Section Waste Removal System during trimming with the<br />
magnetized nozzle, or during extensive cleaning with the<br />
flexible hose. Solid waste is collected in a concealed primary<br />
filter system and air is filtered through a HEPA filter for<br />
added safety in the laboratory environment. Liquid condensate<br />
is collected in a condensate bottle for safer disposal.
The ergonomic design of the Leica CM1950 protects the user<br />
from sharp edges and helps prevent work-related musculoskeletal<br />
disorders (MSD) and repetitive strain injury (RSI).<br />
Rounded edges allow the user to adopt a comfortable working<br />
position. Bright, glare-free illumination helps reduce eye<br />
fatigue.<br />
The new disposable blade holder CE with integrated, colored<br />
safety guard and blade removal aid makes blade handling<br />
safer. For users who prefer using the brush technique during<br />
sectioning, the palm rest can be used instead of the anti-roll<br />
guide.<br />
For individual user comfort, the Leica chair and height<br />
adjustable foot rest are available as optional accessories.<br />
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Simple specimen identification using the optional, color-coded specimen discs<br />
Fast, efficient specimen orientation with clearly defined zero position<br />
Disposable blade holder for both high and low profile blades<br />
Frost-free, enclosed microtome assembly<br />
Optional motor drive for specimen sectioning<br />
THE cryostats<br />
for highly efficient workflow<br />
The best workflow for efficient operation is achieved by:<br />
Certified UVC disinfection at low temperatures – does not produce condensation or<br />
contaminated waste, is effective at low temperatures, and can be terminated at any<br />
time to process urgent cases<br />
Easy for the multi users: the selfexplanatory,<br />
single-function keys and<br />
easily readable LEDs help prevent<br />
operating errors, facilitate cryosectioning,<br />
and make scrolling through<br />
multiple menus unnecessary. The<br />
control panels are comfortably positioned<br />
in the armrests to eliminate<br />
the need to reach into the cold chamber<br />
to use or adjust settings.<br />
Tools, glass slides, staining containers,<br />
etc. can be stored in a recessed<br />
area on top of the cryostat to keep<br />
them immediately at hand.
Sectioning tools/aids etc. can be conveniently placed on<br />
the optional, movable shelf.<br />
The specimen discs can be pre-cooled in the optional<br />
internal storage container, and then easily organized and<br />
transported.<br />
Optional color coded specimen discs help identify specimens<br />
coming from multiple sources. The specimen disc<br />
features a visual specimen orientation aid to enable precise<br />
orientation.<br />
A clearly defined zero-position allows faster<br />
specimen alignment prior to sectioning and<br />
easy realignment if the specimen is removed<br />
from the object head.<br />
With the new disposable blade holder CE blade<br />
exchange is simple and fast. The holder’s lateral<br />
displacement function allows the use of the full<br />
length of the blade. Small and large sections are<br />
easily lifted from the large, horizontal pressure<br />
plate.<br />
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Leica CM1950 Technical Specifications<br />
The Leica CM1950 basic instrument is equipped with CryoZone technology, UVC Disinfection, specimen orientation and AgProtect<br />
antimicrobial nanosilver coating.<br />
An individual, tailor-made instrument can be configured by use of the various options available:<br />
Actively cooled object head (double compressor)<br />
Vacuum Section Waste Removal System<br />
Motorized Sectioning<br />
A wide range of accessories<br />
UVC surface disinfection: 30 or 180 minutes, user selectable<br />
Microtome<br />
Section thickness range: Setting values: 1 to 100 µm<br />
1.0 µm – 5.0 µm in 0.5 µm steps<br />
5.0 µm – 20.0 µm in 1.0 µm steps<br />
20.0 µm – 60.0 µm in 5.0 µm steps<br />
60.0 µm – 100.0 µm in 10.0 µm steps<br />
Trimming range: Clinical: 10 – 40 µm<br />
Setting values: 10 µm, 20 µm, 30 µm, 40 µm<br />
Research: 1 – 600 µm<br />
Setting values: 1,0 µm – 10,0 µm in 1,0 µm steps<br />
10,0 µm – 20,0 µm in 2,0 µm steps<br />
20,0 µm – 50,0 µm in 5,0 µm steps<br />
50,0 µm – 100,0 µm in 10,0 µm steps<br />
100,0 µm – 600,0 µm in 50,0 µm steps<br />
Maximum specimen size: 50 x 80 mm<br />
Total specimen feed: 25 mm<br />
Vertical specimen stroke: 59 mm<br />
Specimen retraction: 20 µm or off<br />
Specimen orientation: 8° (x-, y-axis), 360° rotation of specimen disc<br />
Electric coarse feed: Slow: 300 µm/s, in 20 µm steps<br />
Fast: 900 µm/s<br />
Cryochamber<br />
Temperature range: 0°C to -35°C at ambient temperature of 20 °C<br />
Specimen cooling (optional)<br />
Temperature range: – 10 to – 50 °C<br />
Defrosting of specimen head: manual defrost<br />
Cryochamber defrosting<br />
Automatic cryochamber defrosting: programmable, (hot gas defrost),<br />
selectable time,<br />
1 defrost in 24 h or manual hot gas defrost,<br />
defrost time: 12 minutes<br />
Automatic shut off defrost: at – 5 °C chamber temperature
Quick-freeze shelf<br />
Minimum temperature: down to – 42 °C, at chamber temp. – 35°C<br />
Number of freezing stations: 15 + 2<br />
Defrost: manual hot-gas defrost<br />
Peltier element<br />
Number of freezing stations: 2<br />
Maximum temperature difference: 17 K, at chamber temp. of – 35 °C<br />
Power draw 1900 VA<br />
General information<br />
Operating temperature range: 18°C to 35°C<br />
Relative humidity: max. 60%, non-condensing<br />
Storage humidity: < 60%<br />
Dimensions and Weights<br />
Width (without handwheel): 700 mm<br />
Width (with handwheel): 835 mm<br />
Depth (cabinet only): 850 mm<br />
Height (total): 1215 mm<br />
Working height (armrest): 1025 mm<br />
Weight: 165 kg<br />
Weight (with vacuum): 183 kg<br />
Weight (with motor): 185 kg<br />
Weight (with motor and vacuum): 193 kg<br />
Choice of blade or knife holders:<br />
Stable blade holder base with segment arc for lateral displacement of the blade.<br />
Disposable blade holder insert CE for high and low profile blades with 2 anti-roll guides with 50 and 100 µm spacer with glare<br />
minimizing glass insert, colored safety guard and blade removal help. Palm rest for brush technique.<br />
Stable knife holder base for knife holder CN or knife holder CNZ. Knife holder CN for reusable steel knives<br />
Knife holder CNZ for reusable steel or tungsten carbide knives with central pressure plate for full usage of the knife.<br />
Wide range of accessories available on request.<br />
Technical specifications subject to change without prior notice.<br />
As confirmed by the successful c_CSA_us certification, the Leica CM1950<br />
has been designed and manufactured in compliance with US, Canadian and IEC<br />
requirements.<br />
State-of-the-art development, manufacturing and quality control procedures –<br />
certified under DIN EN ISO 9001 – ensure highest quality and reliability.<br />
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