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<strong>Wolf</strong> <strong>Laboratories</strong> Limited<br />

www.wolflabs.co.uk<br />

Tel: 01759 301142 Fax:01759 301143 sales@wolflabs.co.uk<br />

Use the above details to contact us if this literature doesn't answer all your<br />

questions.<br />

Pricing on any accessories shown can be found by keying the part number<br />

into the search box on our website.


Leica CM1950 Configuration Europe<br />

WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />

specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />

AgProtect nanosilver coating, disposable blade holder CE.<br />

Instrument features:<br />

Freestanding cryostat with encapsulated, splashproof microtome. Spacious,<br />

stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />

disinfect. Heated, removable sliding window. Stable, selfcontained<br />

cryocabinet on rollers. Collection container for condensing water at front of<br />

instrument. Outer surfaces and controls of instrument coated with AgProtect<br />

nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />

two positions. Easytohandle and stable clamping system for clamping the<br />

specimen discs. 8 ° XY specimen orientation with zero point reference.<br />

Specimen retraction for manual mode of operation, (20 m), can be switched<br />

off.<br />

Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />

Disinfection can be aborted at any time, if work needs to be resumed<br />

immediately. Automatic safety cut-off of disinfection cycle when sliding<br />

window is opened.<br />

Twin compressor cooling system for cryochamber and specimen head.<br />

Cooling system with safety pressure control. Specimen temperature<br />

selectable from -10°C to -50°C in 1 ° steps.<br />

Cryochamber temperature selectable from 0 °C to<br />

• 35 °C, +5K, adjustable in 1K increments atambient temperature of 20<br />

°C.<br />

Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />

shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />

Peltier element freezing stations (temperatures down to -52°C) with manual<br />

defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />

every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />

minutes.<br />

8


WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />

elements and specimen head with acoustic warning signal to prevent<br />

unintentional defrosting. Manual defrost cycle for Chamber and quick-freezing<br />

shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />

Low-maintenance microtome with cross roller guides. Reproducible highquality<br />

thin sections via stepper motor specimen feed. Section thickness<br />

selection from outside the cryochamber.<br />

Section thickness selection:<br />

Trimming thickness range: selectable via key combination when instrument is<br />

being switched on.<br />

For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />

For research applications: 1600 m:<br />

selectable in 1 m steps from 110 m<br />

selectable in 2 m steps from 1020 m,<br />

selectable in 5 m steps from 2050 m<br />

selectable in 10 m steps from 50100 m<br />

selectable in 50 m steps from 100600 m.<br />

Sectioning thickness range: 1100 m,<br />

selectable in 0.5 m steps from 15 m<br />

selectable in 1 m steps from 520 m<br />

selectable in 5 m steps from 2060 m<br />

selectable in 10 m steps from 60100 m.<br />

Total vertical specimen stroke: 59 mm<br />

Total horizontal specimen feed: 25 mm<br />

Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />

Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />

Control panel with foil-protected buttons and locking function.<br />

Self-explanatory symbols for all essential functions and displays.<br />

LEDdisplay for cryochamber and specimen head temperature, actual time,<br />

defrost time.<br />

Visual indication of specimen stop positions (front/home).<br />

Blade holder base with segment arc for blade holder CE for high and low<br />

profile disposable blades. Blade holder CE for high and low profile blades with<br />

lateral adjustment, clearance angle adjustment and integrated knife guard<br />

with blade ejection lever.<br />

Manufactured in compliance with cCSAUS and CE standards.<br />

CFCfree.<br />

Technical data:<br />

• Mains power supply: 230 V/50 Hz (European version)<br />

• Admissions: c_CSA_US, CE<br />

• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />

27.55 (with handwheel: 2.87) x 33.46 x 47.84 inches<br />

• Weight: 175 kg / 385.46 lbs<br />

9


WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

All specifications related to temperature are valid for a room temperature up to<br />

20 °C and a relative air humidity of 60%.<br />

Operating temperature range: +18 °C to 35 °C<br />

Standard delivery:<br />

• 1 Basic instrument (14 0477 42456)<br />

• 1 Handwheel, assy. manual (14 0477 41346)<br />

• 1 Mains cable EU 230 V/16A (14 0411 32570)<br />

• 5 Specimen discs, 30 mm (14 0477 40044)<br />

• 1 Section waste tray (14 0477 40062)<br />

• 1 Retaining device for quick-freezing shelf (14 0477 40080)<br />

• 1 <strong>Cover</strong> for quickfreezing shelf (14 0477 43763)<br />

• 1 Tool set (14 0436 43463):<br />

• 1 Brush, fine (14 0183 28642)<br />

• 1 Leica brush with magnet (14 0183 40426)<br />

• 1 Allen key with handle, size 5 (14 0194 04760)<br />

• 1 Allen key, size 2.5 (14 0222 04137)<br />

• 1 Allen key, size 3.0 (14 0222 04138)<br />

• 1 Allen key, size 4.0 (14 0222 04139)<br />

• 1 Allen key, size 5.0 (14 0222 04140)<br />

• 1 Allen key, size 6.0 (14 0222 04141)<br />

• 1 Allen key, size 1.5 (14 0222 10050)<br />

• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />

• 1 Single-head wrench, size 16 (14 0330 18595)<br />

• 1 Singlehead wrench, size 13 (14 0330 33149)1 Bottle of cryostat oil,<br />

type 407, 50 ml (14 0336 06098) 1 Tissue freezing medium for<br />

cryosectioning, 125 ml (14 0201 08926) 1 Pair of safety gloves, size<br />

"M" for cryocsectioning (14 0340 29011) 1 Blade holder base with<br />

segment arc (14 0477 40351) 1 Disposable blade holder (14 0477<br />

43005) consisting of:<br />

• insert for lowprofile blades (14 0477 40386)<br />

• finger rest for brush technique (14 0477 40389)<br />

• antiroll plate, 70 mm wide, with 50 m spacer and with glare-minimizing<br />

glass insert (14 0477 42491)<br />

• antiroll plate 70 mm wide, with 100 m spacer and glare-minimizing<br />

glass insert (14 0477 42492) 1 EC Declaration of Conformity (14 0477<br />

80011) 1 Instructions for use Leica CM1950 (14 0477 80001)<br />

No : 1491950C2EU<br />

10


Leica CM1950 Configuration Europe<br />

WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />

specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />

AgProtect nanosilver coating, motor for motorized sectioning, disposable<br />

blade holder CE<br />

Instrument features:<br />

Freestanding cryostat with encapsulated, splash-proof microtome. Spacious,<br />

stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />

disinfect. Heated, removable sliding window. Stable, self-contained<br />

cryocabinet on rollers. Collection container for condensing water at front of<br />

instrument. Outer surfaces and controls of instrument coated with AgProtect<br />

nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />

two positions. Easytohandle and stable clamping system for clamping the<br />

specimen discs. 8° XY specimen orientation with zero point reference.<br />

Specimen retraction for manual mode of operation, (20 m), can be switched<br />

off. Motorized modes of operation equipped with selfadjusting retraction<br />

system, retraction value depending on sectioning speed.<br />

Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />

Disinfection can be aborted at any time, if work needs to be resumed<br />

immediately. Automatic safety cutoff of disinfection cycle when sliding window<br />

is opened.<br />

Motorized sectioning, single and continuous stroke. Motorized sectioning<br />

operated via control panel or optional foot switch. When motorized sectioning<br />

is stopped via the control panel, the handwheel is automatically locked in the<br />

bottom position. Safety handwheel can be centered during motorized<br />

sectioning. 2 Sectioning speed ranges selectable: 050 strokes per minute or<br />

085 strokes per minute. Vmax button for fast trimming: 85 to 90 strokes per<br />

minute.<br />

Twin compressor cooling system for cryochamber and specimen head.<br />

Cooling system with safety pressure control. Specimen temperature<br />

selectable from -10°C to -50°C in 1 ° steps.<br />

Cryochamber temperature selectable from 0 °C to<br />

11


WOLF LABS<br />

Think sustainable<br />

• 35 °C, +5K, adjustable in 1K increments at ambient temperature of<br />

20°C.<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />

shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />

Peltier element freezing stations (temperatures down to -52°C) with manual<br />

defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />

every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />

minutes.<br />

Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />

elements and specimen head with acoustic warning signal to prevent<br />

unintentional defrosting. Manual defrost cycle for Chamber and quickfreezing<br />

shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />

Low-maintenance microtome with cross roller guides. Reproducible high<br />

quality thin sections via stepper motor specimen feed. Section thickness<br />

selection from outside the cryochamber.<br />

Section thickness selection:<br />

Trimming thickness range: selectable via key combination when instrument is<br />

being switched on.<br />

For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />

For research applications: 1600 m:<br />

selectable in 1 m steps from 110 m<br />

selectable in 2 m steps from 1020 m,<br />

selectable in 5 m steps from 2050 m<br />

selectable in 10 m steps from 50100 m<br />

selectable in 50 m steps from 100600 m.<br />

Sectioning thickness range: 1100 m,<br />

selectable in 0.5 m steps from 15 m<br />

selectable in 1 m steps from 520 m<br />

selectable in 5 m steps from 2060 m<br />

selectable in 10 m steps from 60100 m.<br />

Total vertical specimen stroke: 59 mm<br />

Total horizontal specimen feed: 25 mm<br />

Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />

Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />

Control panel with foil-protected buttons and locking function.<br />

Self-explanatory symbols for all essential functions and displays.<br />

LEDdisplay for cryochamber and specimen head temperature, actual time,<br />

defrost time. Visual indication of specimen stop positions (front/home).<br />

Blade holder base with segment arc for blade holder Ce for high and low<br />

profile disposable blades.<br />

Blade holder CE for high and low profile blades with lateral adjustment,<br />

clearance angle adjustment and integrated knife guard with blade ejection<br />

lever. Manufactured in compliance with cCSAUS and CE standards.<br />

CFCfree.<br />

12


WOLF LABS<br />

Think sustainable<br />

Technical data:<br />

• Mains power supply: 230 V/50 Hz (European version)<br />

• Admissions: c_CSA_US, CE<br />

• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />

27.55 (with handwheel: 32.87) x 33.46 x 47.84 inches<br />

• Weight: 185 kg / 407.49 lbs<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

All specifications related to temperature are valid for a room temperature up to<br />

20 °C and a relative air humidity of 60%.<br />

Operating temperature range: +18 °C to 35 °C<br />

Standard delivery:<br />

1 Basic instrument (14 0477 42458)<br />

1 Handwheel, assy. motorized (14 0477 41347)<br />

1 Mains cable EU 230 V/16A (14 0411 32570)<br />

5 Specimen discs, 30 mm (14 0477 40044)<br />

1 Section waste tray (14 0477 40062)<br />

1 Retaining device for quick-freezing shelf (14 0477 40080)<br />

1 <strong>Cover</strong> for quick-freezing shelf (14 0477 43763)<br />

1 Tool set (14 0436 43463):<br />

• 1 Brush, fine (14 0183 28642)<br />

• 1 Leica brush with magnet (14 0183 40426)<br />

• 1 Allen key with handle, size 5 (14 0194 04760)<br />

• 1 Allen key, size 2.5 (14 0222 04137)<br />

• 1 Allen key, size 3.0 (14 0222 04138)<br />

• 1 Allen key, size 4.0 (14 0222 04139)<br />

• 1 Allen key, size 5.0 (14 0222 04140)<br />

• 1 Allen key, size 6.0 (14 0222 04141)<br />

• 1 Allen key, size 1.5 (14 0222 10050)<br />

• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />

• 1 Single-head wrench, size 16 (14 0330 18595)<br />

• 1 Single-head wrench, size 13 (14 0330 33149)1 Foot switch dummy<br />

(14 0443 30420) 1 Bottle of cryostat oil, type 407, 50 ml (14 0336<br />

06098) 1 Tissue freezing medium for cryosectioning, 125 ml (14 0201<br />

08926) 1 Pair of safety gloves, size "M" for cryocsectioning (14 0340<br />

29011) 1 Blade holder base with segment arc (14 0477 40351) 1<br />

Disposable blade holder (14 0477 43005) consisting of:<br />

• insert for low profile blades (14 0477 40386)<br />

• finger rest for brush technique (14 0477 40389)<br />

• antiroll plate 70 mm wide, with 50 m spacer,with glare-minimizing<br />

glass insert (14 0477 42491)<br />

• antiroll plate 70 mm wide, with 100 m spacer,with glare-minimizing<br />

glass insert (14 0477 42492)<br />

• 1 EC Declaration of Conformity (14 0477 80011) 1 Instructions for use<br />

Leica<br />

• CM1950 (14 0477 80001)<br />

No : 1491950C4EU<br />

13


Leica CM1950 Configuration Europe<br />

WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Leica CM1950 Configuration Europe 230V/50 Hz with cryochamber and<br />

specimen cooling, deactivable specimen retraction, certified UVC disinfection,<br />

AgProtect nanosilver coating, vacuum system and motor for motorized<br />

sectioning. with blade holder CE<br />

Instrument features:<br />

Freestanding cryostat with encapsulated, splash-proof microtome. Spacious,<br />

stainlesssteel cryochamber, with antiglare illumination. Easy to clean and<br />

disinfect. Heated, removable sliding window. Stable, selfcontained<br />

cryocabinet on rollers. Collection container for condensing water at front of<br />

instrument. Outer surfaces and controls of instrument coated with AgProtect<br />

nanosilver coating to reduce risk of infection. Handwheel manually lockable in<br />

two positions. Easy-to-handle and stable clamping system for clamping the<br />

specimen discs. 8 ° XY specimen orientation with zero point reference.<br />

Specimen retraction for manual mode of operation, (20 m), can be switched<br />

off. Motorized modes of operation equipped with self-adjusting retraction<br />

system, retraction value depending on sectioning speed.<br />

Certified UVC disinfection: 30 and 180minute cycles can be selected.<br />

Disinfection can be aborted at any time, if work needs to be resumed<br />

immediately. Automatic safety cutoff of disinfection cycle when sliding window<br />

is opened.<br />

Vacuum system for stretching the sections and for removal of section debris,<br />

with saving integrated filter system and bacteria filter on the outside of the<br />

instrument.<br />

Motorized sectioning, single and continuous stroke. Motorized sectioning<br />

operated via control panel or optional foot switch. When motorized sectioning<br />

is stopped via the control panel, the handwheel is automatically locked in the<br />

bottom position. Safety handwheel can be centered during motorized<br />

sectioning. 2 Sectioning speed ranges selectable: 050 strokes per minute or<br />

085 strokes per minute. Vmax button for fast trimming: 85 to 90 strokes per<br />

minute.<br />

14


WOLF LABS<br />

Think sustainable<br />

Twin compressor cooling system for cryochamber and specimen head.<br />

Cooling system with safety pressure control. Specimen temperature<br />

selectable from -10°C to -50°C in 1 ° steps. Cryochamber temperature<br />

selectable from 0°C to<br />

• 35 °C,+5K, adjustable in 1K increments at ambient temperature of 20<br />

°C.<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

Easy to clean, actively cooled specimen preparation zone with quick-freezing<br />

shelf for up to 15 specimens (temperature selection down to -42°C) plus 2<br />

Peltier element freezing stations (temperatures down to -52°C) with manual<br />

defrost cycle. Cryochamber defrosted via automatic hotgas defrosting once<br />

every 24 hours, programmable in 15minute increments. Defrost cycle: 12<br />

minutes.<br />

Manual defrosting of cryochamber, quick-freezing shelf with integrated Peltier<br />

elements and specimen head with acoustic warning signal to prevent<br />

unintentional defrosting. Manual defrost cycle for Chamber and quick-freezing<br />

shelf with Peltier elements: 12 minutes for specimen head: 15 minutes.<br />

Low-maintenance microtome with cross roller guides. Reproducible high<br />

quality thin sections via stepper motor specimen feed. Section thickness<br />

selection from outside the cryochamber.<br />

Section thickness selection:<br />

Trimming thickness range: selectable via key combination when instrument is<br />

being switched on.<br />

For clinical applications: 4 trimming thickness settings: 10, 20, 30 and 40 m.<br />

For research applications: 1600 m:<br />

selectable in 1 m steps from 110 m<br />

selectable in 2 m steps from 1020 m,<br />

selectable in 5 m steps from 2050 m<br />

selectable in 10 m steps from 50100 m<br />

selectable in 50 m steps from 100600 m.<br />

Sectioning thickness range: 1100 m,<br />

selectable in 0.5 m steps from 15 m<br />

selectable in 1 m steps from 520 m<br />

selectable in 5 m steps from 2060 m<br />

selectable in 10 m steps from 60100 m.<br />

Total vertical specimen stroke: 59 mm<br />

Total horizontal specimen feed: 25 mm<br />

Motorized coarse feed in 2 speeds: 300 m/s or 900 m/s.<br />

Step function: 20 m each time the key is pressed at slow coarse feed speed.<br />

Control panel with foil-protected buttons and locking function.<br />

Self-explanatory symbols for all essential functions and displays.<br />

LEDdisplay for cryochamber and specimen head temperature, actual time,<br />

defrost time.<br />

Visual indication of specimen stop positions (front/home).<br />

15


WOLF LABS<br />

Think sustainable<br />

Blade holder base with segment arc for blade holder CE for high and low<br />

profile disposable blades.<br />

Blade holder CE for high and low profile blades with lateral adjustment,<br />

clearance angle adjustment and integrated knife guard with blade ejection<br />

lever.<br />

Manufactured in compliance with cCSAUS and CE standards.<br />

CFCfree.<br />

Technical data:<br />

• Mains power supply: 230 V/50 Hz (European version)<br />

• Admissions: c_CSA_US, CE<br />

• Dimensions (WxDxH):700 (with handwheel 835) x 850 x 1215mm<br />

27.55 (with handwheel: 32.87) x 33.46 x 47.84 inches<br />

• Weight: 193 kg / 425.50 lbs<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

All specifications related to temperature are valid for a room temperature up to<br />

20 °C and a relative air humidity of 60%.<br />

Operating temperature range: +18 °C to 35 °C<br />

Standard delivery:<br />

1 Basic instrument (14 0477 42459)<br />

1 Handwheel, assy. motorized (14 0477 41347)<br />

1 Mains cable EU 230 V/16A (14 0411 32570)<br />

1 Extraction accessory set (14 0477 43300):<br />

• 1 Hose adapter 1 (14 0477 40293)<br />

• 1 Hose adapter 2 (0477 40294)<br />

• 1 Extraction nozzle (14 0477 40295)<br />

• 1 Silicon hose (14 0477 43302)<br />

• 1 Silicon stopper (14 0477 43304)<br />

• 1 Cryochamber extraction nozzle (14 0477 43779)<br />

• 1 Set of filters (5 pcs) (14 0477 43792)5 Specimen discs, 30mm (14<br />

0477 40044) 1 Section waste tray (14 0477 40062) 1 Retaining device<br />

for quick-freezing shelf (14 0477 40080) 1 <strong>Cover</strong> for quick-freezing<br />

shelf (14 0477 43763) 1 Tool set (140436 43463):<br />

• 1 Brush, fine (14 0183 28642)<br />

• 1 Leica brush with magnet (14 0183 40426)<br />

• 1 Allen key with handle, size 5 (14 0194 04760)<br />

• 1 Allen key, size 2.5 (14 0222 04137)<br />

• 1 Allen key, size 3.0 (14 0222 04138)<br />

• 1 Allen key, size 4.0 (14 0222 04139)<br />

• 1 Allen key, size 5.0 (14 0222 04140)<br />

• 1 Allen key, size 6.0 (14 0222 04141)<br />

• 1 Allen key, size 1.5 (14 0222 10050)<br />

• 1 Allen key, size 4 with spherical head(14 0222 32131)<br />

• 1 Single-head wrench, size 16 (14 0330 18595)<br />

• 1 Single-head wrench, size 13 (14 0330 33149)1 Foot switch dummy<br />

(14 0443 30420) 1 Bactericidal filter 350/5865 (140477 40296) 1 Bottle<br />

of cryostat oil, type 407, 50 ml (14 0336 06098) 1 Tissue freezing<br />

16


WOLF LABS<br />

Think sustainable<br />

Tel: 01759 301142<br />

Fax: 01759 301143<br />

E-mail: sales@wolflabs.co.uk<br />

medium for cryosectioning, 125 ml (14 0201 08926) 1 Pair of safety<br />

gloves, size "M" for cryocsectioning (14 0340 29011) 1 Blade holder<br />

base with segment arc (14 0477 40351) 1 Disposable blade holder (14<br />

0477 43005) consisting of:<br />

• insert for low profile blades (14 0477 40386)<br />

• finger rest for brush technique (14 0477 40389)<br />

• antiroll plate, 70 mm wide, with 50 m spacerand with glare-minimizing<br />

glass insert (14 0477 42491)<br />

• antiroll plate 70 mm wide, with 100 m spacerand glare-minimizing<br />

glass insert (14 0477 42492) 1 EC Declaration of Conformity (14 0477<br />

80011) 1 Instructions for use Leica CM1950 (14 0477 80001)<br />

No : 1491950C5EU<br />

17


Leica CM1950<br />

THE Disinfecting Clinical Cryostats


Leica CM1950 Platform<br />

THE cryostats for high quality sectioning, user safety and efficient workflow<br />

The Leica CM1950 platform is the latest innovation in a long line of distinguished<br />

cryostats manufactured by Leica Microsystems. Leica designed and developed the<br />

new family of cryostats based upon users’ feedback and their requirements for<br />

high quality sections, user safety, and efficient laboratory workflow.<br />

High Quality Sectioning<br />

2<br />

High precision microtome with stepper motor object feed<br />

Motorized sectioning (optional)<br />

New stable specimen clamp system<br />

New flat specimen discs for high specimen stability<br />

New rapid-freezing shelf design<br />

New effective object cooling system (optional)<br />

User Safety<br />

UVC disinfection<br />

AgProtect antimicrobial nanosilver surface coating<br />

New disposable blade holder CE<br />

Debris-free, frost-free enclosed microtome assembly<br />

Leica’s Section Waste Removal System (optional)<br />

Spacious cabinet with rounded edges inside and out<br />

Efficient Workflow<br />

UVC disinfection at any time and temperature<br />

Organized work space inside and out<br />

Frost-free, encapsulated microtome assembly<br />

Leica’s Section Waste Removal System with easily-accessible,<br />

concealed filter system (optional)<br />

Easily recognizable control symbols<br />

Visible operating parameters at all times<br />

For an extra measure of safety, the inside of<br />

the instrument features an ozone-free UVC<br />

disinfection system that is certified to be<br />

effective against most bacteria, virus, and fungi,<br />

even at cold temperatures.<br />

Increased safety for the user and others in the<br />

immediate work area: Leica’s AgProtect<br />

antimicrobial nanosilver surface coating<br />

deters the propagation of infectious agents on<br />

the outside of the instrument.<br />

Protect<br />

The brigh<br />

cabinet<br />

allow eff<br />

ple spec<br />

Learn quickly and work confidently<br />

using the externally located,<br />

self-explanatory controls<br />

with easily recognizable symbols.<br />

To protect the laboratory environment from<br />

contamination, a multi-filter system (including<br />

HEPA filter) removes particulates and cleans<br />

the air exhausted from the optional Section<br />

Waste Removal System<br />

Liquid condensa<br />

collected in an ea<br />

ble waste contain


tly lit, ergonomic cryos<br />

spacious enough to<br />

cient handling of multimens.<br />

te is safely<br />

sily accessier.<br />

Store tools, glass slides, staining<br />

containers, etc. in a<br />

recessed area on top of the<br />

cryostat to have these items<br />

immediately at hand.<br />

For extra ease of use and consistent<br />

sectioning of all specimen<br />

types, an optional sectioning<br />

motor drive is available.<br />

Leica Design by Werner Hölbl<br />

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4<br />

NEW: CryoZone blade holder and anti-roll guide cooling<br />

Maintaining the ideal object temperature for specific specimens<br />

Great stability of the microtome and blade holder system<br />

Extreme accuracy of the stepper motor object feed<br />

Consistent, motor-driven cutting stroke (optional)<br />

Vacuum-assisted sectioning aid (optional)<br />

High precision microtome with exact stepper motor object feed.<br />

THE cryostats<br />

for high quality sectioning<br />

Obtain high quality sections through:<br />

CryoZone – the cool way to improve section quality<br />

The CryoZone system controls a zone of uniformly cool air in the critical areas of the cryochamber. This<br />

cool air circulates around the specimen, knife and anti-roll guide to create the optimal conditions for consistent,<br />

high-quality sectioning.


Efficient specimen loading is achieved via single lever<br />

operation that provides maximum clamping force independent<br />

of individual user strength.Direct contact cooling<br />

through the (optional) actively cooled object head facilitates<br />

rapid changes of specimen temperatures to provide<br />

the best conditions for sectioning various specimen types.<br />

High specimen stability is achieved by adherence to deeply<br />

grooved specimen discs. Specimens freeze quickly<br />

because the pre-cooled discs feature a large back surface<br />

that fully contacts the freezing shelf with integrated Peltier<br />

element.<br />

Superior sectioning of routine, hard, and/or large samples is<br />

achieved using a choice of knife/blade holders as well as the<br />

new dove tail mounting system that provides increased stability<br />

and extended working distance. The new CE holder for<br />

high and low profile disposable blades evenly distributes<br />

clamping pressure across the entire length of the blade,<br />

which provides vibration-free sectioning.<br />

The new CN and CNZ knife holders are available for steel<br />

or tungsten carbide knives (optional accessories).<br />

The optional vacuum sectioning aid achieves time-saving<br />

section preparation and reduces section curling.<br />

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Histology laboratorires continue to express major concerns<br />

about biohazard exposure and non-ergonomic work practices.<br />

As a result of their feedback, the Leica CM1950 family of<br />

cryostats is designed to provide maximum protection from these<br />

risks at all stages of the sectioning process.<br />

6<br />

Certified UVC disinfection of exposed surfaces and air<br />

within the cryochamber at cold temperatures<br />

AgProtect antimicrobial nanosilver coating on the<br />

outside surfaces of the cryostat<br />

Optional Section Waste Removal System with a series of<br />

three filters<br />

Ergonomic exterior cabinet design with rounded edges<br />

and comfortable working height<br />

Optional ergonomic chair and foot rest<br />

THE cryostats<br />

for the highest user safety<br />

Leica’s built-in UVC disinfection system provides<br />

certified protection from infectious material and<br />

minimizes the risk of contamination in the cryochamber.<br />

Scientific studies prove that surface<br />

disinfection from bacteria, viruses, and fungi can<br />

be achieved with UVC light. Certification proving the effectiveness<br />

of UVC disinfection against a range of microorganisms can be<br />

downloaded from www.leica-microsystems.com/cm1950_safety,<br />

which includes detailed information on the test procedure and the<br />

efficacy of UVC disinfection.<br />

To efficiently reduce the propagation of infectious agents on<br />

the outside surfaces of the cryostat, the Leica CM1950 family<br />

features an antimicrobial nanosilver surface coating, called<br />

AgProtect. Silver (Ag) ions are well documented<br />

for their ability to reduce bacterial growth.<br />

Protect<br />

Section waste is easily removed by using Leica’s optional<br />

Section Waste Removal System during trimming with the<br />

magnetized nozzle, or during extensive cleaning with the<br />

flexible hose. Solid waste is collected in a concealed primary<br />

filter system and air is filtered through a HEPA filter for<br />

added safety in the laboratory environment. Liquid condensate<br />

is collected in a condensate bottle for safer disposal.


The ergonomic design of the Leica CM1950 protects the user<br />

from sharp edges and helps prevent work-related musculoskeletal<br />

disorders (MSD) and repetitive strain injury (RSI).<br />

Rounded edges allow the user to adopt a comfortable working<br />

position. Bright, glare-free illumination helps reduce eye<br />

fatigue.<br />

The new disposable blade holder CE with integrated, colored<br />

safety guard and blade removal aid makes blade handling<br />

safer. For users who prefer using the brush technique during<br />

sectioning, the palm rest can be used instead of the anti-roll<br />

guide.<br />

For individual user comfort, the Leica chair and height<br />

adjustable foot rest are available as optional accessories.<br />

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8<br />

Simple specimen identification using the optional, color-coded specimen discs<br />

Fast, efficient specimen orientation with clearly defined zero position<br />

Disposable blade holder for both high and low profile blades<br />

Frost-free, enclosed microtome assembly<br />

Optional motor drive for specimen sectioning<br />

THE cryostats<br />

for highly efficient workflow<br />

The best workflow for efficient operation is achieved by:<br />

Certified UVC disinfection at low temperatures – does not produce condensation or<br />

contaminated waste, is effective at low temperatures, and can be terminated at any<br />

time to process urgent cases<br />

Easy for the multi users: the selfexplanatory,<br />

single-function keys and<br />

easily readable LEDs help prevent<br />

operating errors, facilitate cryosectioning,<br />

and make scrolling through<br />

multiple menus unnecessary. The<br />

control panels are comfortably positioned<br />

in the armrests to eliminate<br />

the need to reach into the cold chamber<br />

to use or adjust settings.<br />

Tools, glass slides, staining containers,<br />

etc. can be stored in a recessed<br />

area on top of the cryostat to keep<br />

them immediately at hand.


Sectioning tools/aids etc. can be conveniently placed on<br />

the optional, movable shelf.<br />

The specimen discs can be pre-cooled in the optional<br />

internal storage container, and then easily organized and<br />

transported.<br />

Optional color coded specimen discs help identify specimens<br />

coming from multiple sources. The specimen disc<br />

features a visual specimen orientation aid to enable precise<br />

orientation.<br />

A clearly defined zero-position allows faster<br />

specimen alignment prior to sectioning and<br />

easy realignment if the specimen is removed<br />

from the object head.<br />

With the new disposable blade holder CE blade<br />

exchange is simple and fast. The holder’s lateral<br />

displacement function allows the use of the full<br />

length of the blade. Small and large sections are<br />

easily lifted from the large, horizontal pressure<br />

plate.<br />

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10<br />

Leica CM1950 Technical Specifications<br />

The Leica CM1950 basic instrument is equipped with CryoZone technology, UVC Disinfection, specimen orientation and AgProtect<br />

antimicrobial nanosilver coating.<br />

An individual, tailor-made instrument can be configured by use of the various options available:<br />

Actively cooled object head (double compressor)<br />

Vacuum Section Waste Removal System<br />

Motorized Sectioning<br />

A wide range of accessories<br />

UVC surface disinfection: 30 or 180 minutes, user selectable<br />

Microtome<br />

Section thickness range: Setting values: 1 to 100 µm<br />

1.0 µm – 5.0 µm in 0.5 µm steps<br />

5.0 µm – 20.0 µm in 1.0 µm steps<br />

20.0 µm – 60.0 µm in 5.0 µm steps<br />

60.0 µm – 100.0 µm in 10.0 µm steps<br />

Trimming range: Clinical: 10 – 40 µm<br />

Setting values: 10 µm, 20 µm, 30 µm, 40 µm<br />

Research: 1 – 600 µm<br />

Setting values: 1,0 µm – 10,0 µm in 1,0 µm steps<br />

10,0 µm – 20,0 µm in 2,0 µm steps<br />

20,0 µm – 50,0 µm in 5,0 µm steps<br />

50,0 µm – 100,0 µm in 10,0 µm steps<br />

100,0 µm – 600,0 µm in 50,0 µm steps<br />

Maximum specimen size: 50 x 80 mm<br />

Total specimen feed: 25 mm<br />

Vertical specimen stroke: 59 mm<br />

Specimen retraction: 20 µm or off<br />

Specimen orientation: 8° (x-, y-axis), 360° rotation of specimen disc<br />

Electric coarse feed: Slow: 300 µm/s, in 20 µm steps<br />

Fast: 900 µm/s<br />

Cryochamber<br />

Temperature range: 0°C to -35°C at ambient temperature of 20 °C<br />

Specimen cooling (optional)<br />

Temperature range: – 10 to – 50 °C<br />

Defrosting of specimen head: manual defrost<br />

Cryochamber defrosting<br />

Automatic cryochamber defrosting: programmable, (hot gas defrost),<br />

selectable time,<br />

1 defrost in 24 h or manual hot gas defrost,<br />

defrost time: 12 minutes<br />

Automatic shut off defrost: at – 5 °C chamber temperature


Quick-freeze shelf<br />

Minimum temperature: down to – 42 °C, at chamber temp. – 35°C<br />

Number of freezing stations: 15 + 2<br />

Defrost: manual hot-gas defrost<br />

Peltier element<br />

Number of freezing stations: 2<br />

Maximum temperature difference: 17 K, at chamber temp. of – 35 °C<br />

Power draw 1900 VA<br />

General information<br />

Operating temperature range: 18°C to 35°C<br />

Relative humidity: max. 60%, non-condensing<br />

Storage humidity: < 60%<br />

Dimensions and Weights<br />

Width (without handwheel): 700 mm<br />

Width (with handwheel): 835 mm<br />

Depth (cabinet only): 850 mm<br />

Height (total): 1215 mm<br />

Working height (armrest): 1025 mm<br />

Weight: 165 kg<br />

Weight (with vacuum): 183 kg<br />

Weight (with motor): 185 kg<br />

Weight (with motor and vacuum): 193 kg<br />

Choice of blade or knife holders:<br />

Stable blade holder base with segment arc for lateral displacement of the blade.<br />

Disposable blade holder insert CE for high and low profile blades with 2 anti-roll guides with 50 and 100 µm spacer with glare<br />

minimizing glass insert, colored safety guard and blade removal help. Palm rest for brush technique.<br />

Stable knife holder base for knife holder CN or knife holder CNZ. Knife holder CN for reusable steel knives<br />

Knife holder CNZ for reusable steel or tungsten carbide knives with central pressure plate for full usage of the knife.<br />

Wide range of accessories available on request.<br />

Technical specifications subject to change without prior notice.<br />

As confirmed by the successful c_CSA_us certification, the Leica CM1950<br />

has been designed and manufactured in compliance with US, Canadian and IEC<br />

requirements.<br />

State-of-the-art development, manufacturing and quality control procedures –<br />

certified under DIN EN ISO 9001 – ensure highest quality and reliability.<br />

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