- Page 1: EVALUATION AND CHARACTERIZATION OF
- Page 4 and 5: Dr. RP Thrkur Senior Scientist (Pla
- Page 6 and 7: We certify that the thesis entitled
- Page 8 and 9: My docere thanks are also due to Mr
- Page 11: About the bost GENERAL INTRODUCTION
- Page 15 and 16: GCOPE OF INVESTIGATION As biocontro
- Page 17 and 18: Materials and Methods (General)
- Page 19 and 20: Chapter I Isolation and Evaluation
- Page 21 and 22: The present study was undertaken to
- Page 23 and 24: allowed TrrcMm spp to develop 9n th
- Page 25 and 26: Irdation of Trichoderma isolates MA
- Page 27: RESULTS Two hundred and twelve Tric
- Page 30 and 31: FIG 3 kivphdi ( o11111q of / I ~c ~
- Page 32 and 33: DISCUSSION Quantitative estimation
- Page 34 and 35: Chapter I1 lcharacterization of Tri
- Page 36 and 37: morphological similarities behvan t
- Page 38 and 39: REMEW OF LITERATURE Rr-gsaus Tnchod
- Page 40 and 41: suns to examine rdationships within
- Page 42: Morphdogirrl Cbamcterization. MATEF
- Page 45 and 46: extracted with IS ml of urtraction
- Page 47 and 48: whoresis method was preferable than
- Page 49 and 50: selective sequence PCK reactions we
- Page 51 and 52: Gd Andysis PAGE Cleaning and assemb
- Page 53 and 54: W e s were removed by running with
- Page 55 and 56: TLe reaction was canied out in a PT
- Page 57 and 58: Wer throughout the staining process
- Page 59 and 60: RESULTS Forty-eight antagonistic Tr
- Page 61 and 62: Conidiophore branching was arising
- Page 63 and 64:
ellipsoidal , smooth walled and pal
- Page 65 and 66:
12 0 ir~~horierma)rfrle (T 47) show
- Page 67 and 68:
Table 4 Identification of Trichdma
- Page 69 and 70:
AGIM~~I+CTG, 28 &oRI+ TA/M=I+CAC, 4
- Page 72 and 73:
Fig. 13. UPGMA dendrogram obtained
- Page 74 and 75:
part ffom AFLP marker ITS primers w
- Page 76 and 77:
Fig 15 Polyacrylamide gel electroph
- Page 78 and 79:
Fig. 17. Multidimensional scaling o
- Page 80 and 81:
and identified up to species level.
- Page 82 and 83:
(1991sBib). also sub groupad simila
- Page 84 and 85:
Chapter I11 Mechanism of antagonism
- Page 86 and 87:
inducible enzyme ~ccreted by many m
- Page 88 and 89:
liquid medium were able to induce c
- Page 90 and 91:
Among 35 6 of viride and 7: hurzim
- Page 92 and 93:
- chiti- wryme, which was indicated
- Page 94 and 95:
hpmtion of substrate: Colloidd chit
- Page 96 and 97:
20 min at 37 C in a water bath. OD
- Page 98 and 99:
SDS PAGE Electrophomis Materials 1.
- Page 100 and 101:
ding gd preparation (10%) Distillad
- Page 102 and 103:
preprmtios of SImpk 1. The sample m
- Page 104 and 105:
production of volatile antibiotics.
- Page 106 and 107:
Fig. 19. Effect of diffusible antib
- Page 108 and 109:
Cbi(inue P W ~ by O whO&frn4 ~ Spp.
- Page 110 and 111:
I 18 Kda I 85Kda 5OKda 3 3 Kda 26Kd
- Page 112 and 113:
Table 6. Eff't of Trichodenna dlmib
- Page 114 and 115:
Table 9: specific activity of Gluca
- Page 116 and 117:
Prasd Rangdwaran (2001) tested nine
- Page 118 and 119:
à dl as the ~~* of host pathogen i
- Page 120 and 121:
INTRODUCTION contamination of groun
- Page 122 and 123:
wntents ms M 0dy ~ h i by ~ fobawin
- Page 124 and 125:
RWEW OF LITERATURE ~fl~toh are know
- Page 126 and 127:
appwh. Rammshna el d. (1996) studie
- Page 128 and 129:
- package to redue ~*est seed infec
- Page 130 and 131:
Fig. 22. Biocontrol of A. j7nsr.s c
- Page 132 and 133:
Soil and pod sampling soil and pod
- Page 134 and 135:
~ield evaluation of Trichoderma iso
- Page 136 and 137:
Table 12. Randomization of 8 tmhent
- Page 138 and 139:
~fl~toxin matysis: lndirst competit
- Page 140 and 141:
to rn yeUow. 'l'h.is may sometimes
- Page 142 and 143:
added to each well and inabate for
- Page 144 and 145:
~able,l3. Effect of pH on biomass (
- Page 146 and 147:
Management of .flatoxin contaminati
- Page 148 and 149:
Table IS Population dynamics of bio
- Page 150 and 151:
Fig 25 A typical poit /h of A \per~
- Page 152 and 153:
out of the seven treatments with T~
- Page 154 and 155:
~opulation dynamics of biocontrol a
- Page 156 and 157:
~~fferent isolates of Thdwderma SPP
- Page 158 and 159:
population dynamics of biocontrol a
- Page 160 and 161:
Effect of Trichdnna spP. On pod wei
- Page 162 and 163:
The pathogen populations were drast
- Page 164 and 165:
~ g of ÿ ~ rich t ode^ population
- Page 166 and 167:
Table 23 Effect of Trichodema spp o
- Page 169 and 170:
Matoin produd by Asper@lus flms in
- Page 171 and 172:
apparent in M P ~ ~ckage V ~ plot,
- Page 173 and 174:
poundnut UP to brvest in both rabi
- Page 175 and 176:
~iocontrol potential of various iso
- Page 177 and 178:
~accard algorithm were used for gen
- Page 179 and 180:
agents bnin~i (T 83) and ~iride (T
- Page 181 and 182:
Literature cited
- Page 183 and 184:
British Medical Journal. 2 (5298).
- Page 185 and 186:
Bohn. M.3 Ufl H. F-9 and Me*hinger
- Page 187 and 188:
ChtL 1.9 Harman, G. E,, and Baker,
- Page 189 and 190:
Elad, Y.9 Zviek Y.7 and Chat, 1. 11
- Page 191 and 192:
Harman, G C.7 Chet, and Baker, R. 1
- Page 193 and 194:
Kisyombe C. T, BeUte K., and Payne
- Page 195 and 196:
~ieckfeldt, E., Gary J. Samuels., H
- Page 197 and 198:
Merril, C. R., Goldman, D., Sedman,
- Page 199 and 200:
mold in Europe an* North America to
- Page 201 and 202:
Ridouf C. J.9 Coley.smith, J. R., a
- Page 203 and 204:
Sivan, A , and Che4 11. (1989a). De
- Page 205:
Whik, T. J* T. Btuns.* S. and Jk W.
- Page 208 and 209:
analoxin contamination in groundnur
- Page 210 and 211:
Soil and pod sampling. Soil rvas sa
- Page 212 and 213:
~ ~ ~ l of ~ im ~ Integrated t i ~
- Page 214 and 215:
This could be because of inhibition
- Page 216 and 217:
[AWC~] Trichodem species offer cons
- Page 218 and 219:
3 technique ha m n to hiBhly dirrim
- Page 220 and 221:
- - *- *Rs ~le-eImw ud ICI~~L~R -a
- Page 222 and 223:
-*T.-,T.k~in~m~ij~.-*~rp~~- "*h088.
- Page 224 and 225:
I w ~ ~ ~ . i . D g ~ ~ . ( ~ ~ i f
- Page 226 and 227:
4 Seq- dillgnostic analysis has bee
- Page 228 and 229:
2 ACKNOWLEDGE MEN^ 3 'Ilis ~Upponcd
- Page 230 and 231:
13. - ~ - ~ R a c n b c r L , m d ~
- Page 232:
4 38 R84 M. A- 1969 A ~vtstcm of th
- Page 235 and 236:
TABLE 3 ARP mdcm that d~ffercnl~~lt
- Page 237 and 238:
G T C G A G G T C G A C G T C G A G
- Page 239 and 240:
FIGURE 4A. UPGMA dendrugram of 48 T
- Page 241 and 242:
FIGURE 6a. UPGMA dendrugram b~wd on