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Morphdogirrl Cbamcterization.
MATEFUALS AND METBODS
Morphological characterization was done of 212 Trrchodenno cultures obtained fiom
groundnut himsphere soil samples from major groundnut growing areas of four districts
(Anantapur, Chittoor, Cuddapah, and Kurnool) districts of Andhra Pradesh
and two districts (Kolar and Tumkur) of Karnataka, The morphological characters used
were growth type, growth rate, colony colour, shape of margins, colony diameter,
sporulation and sporulation initiation rate
Specia identification:
Microscopic Observation:
Slldes were prepared from the place of contact of pathogen and the antagonist and
observed for interaction under the microscope
Identification of Trichoderma spp
Tnchdnna spp were grown on 1 5% malt agar for four days and the slides prepared
from the growmg edges Conidia were taken from one-week-old PDA culture
Identification was done with the help of species identification keys (Rifai 1969, Bisset
1984,1991a,b,c, 1992, Samuels (19%) and Nagamani et al., (2002) Fourty eight
antagonistic TncMnna species were attempted to identify to based on branching of
comdiophores, shape of the phialides, emergence of phdospores and shape of the
phidospores (Table 3)