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REMEW OF LITERATURE
Rr-gsaus Tnchodcnna (Asmmycetq Hypoacales) contains speck that uc of great
importance because they produce enzymes, antibiotics, or vr utilized as
bbocrsrd agents Kubid, (1992) Twonomy of Tnchdnw is armntly baaed lugety
in^ wrphological characters (Rifai, 1%9, Bissctt, 1984, 1991kb,c, 1992), such rs
ddd form, sii, color and ornamentation, branching pattern and frsguency of
bmxhg, and the formation of sterile or futile hyphal elongabons From conidiophons
In Trichadenna, isanzyme analysis (Stasz er a1 , 1986, Samuels er a/, 1994,
Leuchtmann el a1 , 1995, Kuhls el a1 , 1995, Bowen er al., 1996, Turoczi et d, 1996), md
rDNA sequencing (Muthumeenakshi el a1 . 1994. Kuhls er a1 . 1996, 1997) have ban
ured to distingu~sh species specific groups of strains within the species that were defined
dtha by common morphology or ecology Recently rDNA was used in comprehensive
survey of two of the sections of the genus that were accepted by (Bissett 1984, 199!a),
the rDNA sequence information provided support for merging the two sections
Moreova, the expanded section 1.ungjbrachiatum included five Hpxrea species and
that the sexual (Hyporrea) and and (Tnchoderma) strains did not reprat rqunte
Lineages but rather represented a single lineage within the ascomycete g ws Hllpmn.
Consequently, we ref7 to Tnchoderma sect hgbrachratum as the Hymcreu
schwemttizii complex (Samuels el al, 1998) AFl,P technology is a DNA fingerprinting
technique that combines both hybridization-based fingerprinting and PCR-based
fingerprinting It is based on the selective amplification of a subset of genomic restriction
.
frngments using PCR Reader and Baroda (1985) described a gened, WIe and
kqmsive method for isolation of DNA from filamentous fungi Starting from fnaa