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suns to examine rdationships within Tricho&mm sed. Lmgbrochrohrm and dated
gleomorphs, Hpoueu schwinitii and H. jecwim Grondo~ el a1 (1997) reported tht
laonoconidial cultures of I5 isolates of TrrcMnna hanrmtum were characterized on
tbe basis of 82 morphological, physidogd, and biochemical htuns and 59 iboezyme
bands from seven enzyme systems and identified four distinct groups Representative
sequences of the ITS I and ITS 2 regon in the ribosomal DNA gene duster were
compared between the groups confirming this distribution The utility of the groupings
generated from the morphological, physiological, and including an additional
environmental isolate in the dearophoretic analysis assessed biochemical data The m
vrtro antibiotic activity of the T. hmrranum isolates was assayed against 10 isolates of
five different soil borne hngal plant pathogens Ospina-Giraldo er d (1998) cxunid
nucleotide sequences of ITS-I and ITS-2 and the 5 8 S region of the ribosomal DNA
repeat in 15 lines of 7i.rchrrlema spp Six lines representing four biotypes of 7:
hmramrm associated with mushroom compost or the raw materials used in the compost
auld be separated into two distinct groups. Ospina-Giraldo el a1 (1999) quenced a
polymerase chain reaction-amplified DNA containing the intend truwaibed apace ITS-
I and ITS-2 regions of the nuclear ribosomal DNA transcriptional unit for 81 isolates of
Trichodenna spp. asnociated with mushroom culture Phylogmtic analysis revealed that
:he biocontrol isolates were more closely related to an isolate of T. hurz~unum biotype 1
:han to the aggressive biotypes 2 and 4 Finally, considering the wide range in sequence
isuibution of our samples, we propose that the consensus sequence found in this
.
nvestigation be used as the reference sequence for FJrther studies involving the
daaification and taxonomy of T. hurziunum (Licckfeldt el al., 1999) based on combined