Fluorescence and Confocal Microscopy

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Detector: Electron Multiplier CCD(EM-CCD) Dangers of Converting From 16 Bit to 8 Bit Hamamatsu Detector: Electron Multiplier CCD(EM-CCD) Nakano, 2002 16 Bit 16 Bit 16 Bit 16 Bit 8 Bit 8 Bit Hamamatsu 10
Starting Images Ending Images 16 Bit 16 Bit 8 Bit 8 Bit References • Douglas C. Prasher, Virginia K. Eckenrode, William W. Ward, Frank G. Prendergast and Milton J. Cormier. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 1992 Feb 15;111(2):229-33. • Chalfie M, Tu Y, Euskirchen G, Ward W, and Prasher DC. Green fluorescent protein as a marker for gene expression. Science. 1994 Feb 11;263(5148):802-5. • Giepmans BN, Adams SR, Ellisman MH, and Tsien RY. The fluorescent toolbox for assessing protein location and function. Science. 2006 Apr 14;312(5771):217-24. • Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, and Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. • Semwogerere D. and Weeks ER. Confocal Microscopy Encyclopedia of Biomaterials and Biomedical Engineering 2005 Nov. 28 10;(1-10). • Nakano A. Spinning-disk confocal microscopy - a cutting-edge tool for imaging of membrane traffic. Cell Struct Funct. 2002 Oct;27(5):349-55. • Nikon Microscopy U – http://www.microscopyu.com/index.html 11
Page 1 and 2: Fluorescence and Confocal Microscop
Page 3 and 4: • Physical Process - Excitation:
Page 5 and 6: Nikon (www.microscopyu.com) Nikon (
Page 7 and 8: Controlling Image Quality: • Spin
Page 9: Diagram of an individual CCD “Wel

Fluorescence and Confocal Microscopy

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