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Fluorescence and Confocal Microscopy

Fluorescence and Confocal Microscopy

Fluorescence and Confocal Microscopy

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Nikon (www.microscopyu.com) Nikon (www.microscopyu.com)<br />

Widefield :<br />

Nikon (www.microscopyu.com)<br />

Widefield :<br />

Point Scanning:<br />

Point Scanning:<br />

Nikon (www.microscopyu.com)<br />

<strong>Confocal</strong> Imaging:<br />

• The invention of the confocal microscope is attributed<br />

to Marvin Minsky, who produced a working<br />

microscope in 1955.<br />

• It was developed to eliminate out-of-focus haze of<br />

fluorescent samples.<br />

5

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