Endogenous allergen assessments - Europabio

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Design of studies is based, in part, on available technology 2 -Serologydetection of allergens with patient IgE or animal IgG antibody -Protein Separation- Visualize individual proteins with stains/serum IgE using electrophoresis as a foundation -Analytical- Mass spectrometry is the newest multiplexing, quantitative methodology
IgE, ug/ml Question: Are Endogenous Allergens in Biotech Crops Increased Relative to Their non-GM Counterparts? ● Has been applied to GM soybean because soybean is considered a major allergenic food ● Traditionally, the technology available was based on serology: 3 - Use soybean allergic sera as a detection reagent for allergen-IgE binding - measure allergen content. • Total anti-soybean IgE binding ELISA – measures IgE that is specific to “all” of the allergens a person may be responsive to; quantitatively, if validated. • Gel Electrophoresis and Western blotting; can observe binding to multiple, individual allergens. Total IgE To discriminate individual allergens by ELISA with serum, individual ELISAs with protein standards would need to be validated – for soybean this would minimally be > 8 separate assays*. Allergen #1 * - University of Nebraska, FARRP allergenonline.org
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Endogenous allergen assessments - Europabio

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