Endogenous allergen assessments - Europabio
Endogenous allergen assessments - Europabio
Endogenous allergen assessments - Europabio
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AQUA® Peptide Analysis by MS to Quantify Proteins<br />
Two different<br />
isotopes to label<br />
amino acids<br />
These labeled<br />
(heavier) peptides<br />
can then act as<br />
peptide<br />
Standards<br />
Isotope labeled<br />
Native peptide<br />
AQUA peptide<br />
8<br />
H 2N<br />
H 2N<br />
HO<br />
C<br />
NH<br />
CH 2<br />
CH 2<br />
CH 2<br />
CH<br />
C O<br />
NH<br />
Arginine (R) – 174.2<br />
13 C6H 14 15 N4O 2 – 184.2<br />
1 2<br />
H 2N<br />
HO<br />
NH 2<br />
CH 2<br />
CH 2<br />
CH 2<br />
CH 2<br />
CH<br />
C O<br />
Lysine (K) – 146.19<br />
13 C6H 14 15 N2O 2 – 154.19<br />
LSAEFGSLR – 978.51 VSDDEFNNYK – 1229.52<br />
LSAEFGSLR – 988.51 VSDDEFNNYK – 1237.52
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