Endogenous allergen assessments - Europabio
Endogenous allergen assessments - Europabio
Endogenous allergen assessments - Europabio
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IgE, ug/ml<br />
Question: Are <strong>Endogenous</strong> Allergens in Biotech Crops<br />
Increased Relative to Their non-GM Counterparts?<br />
● Has been applied to GM soybean because soybean is considered a<br />
major <strong>allergen</strong>ic food<br />
● Traditionally, the technology available was based on serology:<br />
3<br />
- Use soybean allergic sera as a detection reagent for <strong>allergen</strong>-IgE<br />
binding - measure <strong>allergen</strong> content.<br />
• Total anti-soybean IgE binding ELISA – measures IgE that is specific<br />
to “all” of the <strong>allergen</strong>s a person may be responsive to; quantitatively,<br />
if validated.<br />
• Gel Electrophoresis and Western blotting; can observe binding to<br />
multiple, individual <strong>allergen</strong>s.<br />
Total<br />
IgE<br />
To discriminate individual <strong>allergen</strong>s by ELISA with<br />
serum, individual ELISAs with protein standards<br />
would need to be validated – for soybean this<br />
would minimally be > 8 separate assays*.<br />
Allergen #1<br />
* - University of Nebraska, FARRP <strong>allergen</strong>online.org
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