Endogenous allergen assessments - Europabio

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AQUA® Peptide Analysis by MS to Quantify Proteins Two different isotopes to label amino acids These labeled (heavier) peptides can then act as peptide Standards Isotope labeled Native peptide AQUA peptide 8 H 2N H 2N HO C NH CH 2 CH 2 CH 2 CH C O NH Arginine (R) – 174.2 13 C6H 14 15 N4O 2 – 184.2 1 2 H 2N HO NH 2 CH 2 CH 2 CH 2 CH 2 CH C O Lysine (K) – 146.19 13 C6H 14 15 N2O 2 – 154.19 LSAEFGSLR – 978.51 VSDDEFNNYK – 1229.52 LSAEFGSLR – 988.51 VSDDEFNNYK – 1237.52
Aqua® Peptides and Quantifying; How does it work? 9 Seed <strong>allergen</strong> peptide (previously identified) signal Mix label with seed prep mass Labeled peptide standard + mass spectrometry can distinguish them + + Known amount of standard added Ratio gives quantity
Page 1 and 2: Design, relevance and limitations o
Page 3 and 4: IgE, ug/ml Question: Are Endogenous
Page 5 and 6: 2-D Electrophoresis and Western Blo
Page 7: Liquid Chromatography MS/MS for Pro
Page 11 and 12: µg allergen protein/mg total prote
Page 13 and 14: Soybeans 13 Glycine max Yes, it’s
Page 15 and 16: Relevance of the data - Can this da
Page 17: Questions and Discussion ● Thank

Endogenous allergen assessments - Europabio

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