Endogenous allergen assessments - Europabio

Protein Separation and Western Blotting
● Western blotting (WB), and in particular 2-dimensional WB, is of
interest for observing individual proteins
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- A qualitative observation of the proteins binding serum IgE antibody.
• Although “spots” can be optically measured in general, the
technique is considered semi-quantitative since there are
no standard curves assessed for individual allergens (in
most cases).
- Also perceived as beneficial for observing “new” allergens that
may be present in GM variety…
this is improbable given that new varieties are assessed
prior to market release and exposure. There is also no
premise for new proteins, much less new allergens being
created by introduction of GM trait(s).