Endogenous allergen assessments - Europabio

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Protein Separation and Western Blotting ● Western blotting (WB), and in particular 2-dimensional WB, is of interest for observing individual proteins 4 - A qualitative observation of the proteins binding serum IgE antibody. • Although “spots” can be optically measured in general, the technique is considered semi-quantitative since there are no standard curves assessed for individual allergens (in most cases). - Also perceived as beneficial for observing “new” allergens that may be present in GM variety… this is improbable given that new varieties are assessed prior to market release and exposure. There is also no premise for new proteins, much less new allergens being created by introduction of GM trait(s).
2-D Electrophoresis and Western Blotting Total protein stain shown – Red circle indicates observable soybean proteins by Western 5 Gel Electrophoretic pattern Taken from Batista et al., 2007. Int Arch Allergy Immunol 2007;144:29–38 IgE reactivity to all of the possible soybean allergens is very limited in a single serum
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Page 3: IgE, ug/ml Question: Are Endogenous
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Page 15 and 16: Relevance of the data - Can this da
Page 17: Questions and Discussion ● Thank

Endogenous allergen assessments - Europabio

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