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BCS-RD-D-ROCS Method-No. 00857
D-40789 Monheim Report No. MR-099/04
4.1.5 Standard Solutions
Standard solutions (secondary standards) were prepared from the stock solutions by
dilution with acetonitrile/water (2/8, v/v containing 0.22 mL/L formic acid).
Table 3: Preparation Scheme for Reference Standards.
No. Target Concentration (mg/L)
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
A13
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (10 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (1.0 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.1 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.05 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.01 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.005 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.002 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.001 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.0005 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.0001 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.00005 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.00001 each)
[BYI], [Enol], [Ketohydroxy], [Monohydroxy],
[Gluco] (0.000005 each)
Prepared by
Removal of
(mL)
1.95, 2, 1.95,
2.17, 2
*: acetonitrile/water (2/8, v/v, containing 0.22 mL/L formic acid).
No. of
Solution
1, 3, 5, 7,
9
Dilution to
(mL)
Solvent
100 *
10 A1 100 *
1 A1 100 *
0.5 A1 100 *
1 A2 100 *
0.5 A2 100 *
0.2 A2 100 *
0.1 A2 100 *
1 A4 100 *
1 A5 100 *
1 A6 100 *
1 A8 100 *
0.1 A6 100 *