Bayer CropScience AG Page 1 of 82 BCS-RD-D ... - IR-4 Project
Bayer CropScience AG Page 1 of 82 BCS-RD-D ... - IR-4 Project
Bayer CropScience AG Page 1 of 82 BCS-RD-D ... - IR-4 Project
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<strong>Bayer</strong> <strong>CropScience</strong> <strong>AG</strong> <strong>Page</strong> 25 <strong>of</strong> <strong>82</strong><br />
<strong>BCS</strong>-<strong>RD</strong>-D-ROCS Method-No. 00857<br />
D-40789 Monheim Report No. MR-099/04<br />
4.3.4 HPLC Conditions BYI08330-enol-Glc (Individual Determination)<br />
In case <strong>of</strong> interferences (e.g. in orange materials, grape (bunches <strong>of</strong> grape), melon (pulp), hop<br />
(beer)) the following HPLC conditions for the individual determination <strong>of</strong> BYI08330-enol-Glc can<br />
be used:<br />
Instrument: e.g. Agilent 1100<br />
Injector: e.g. HTC PAL, CTC Analytics<br />
Column: e.g. Agilent Zorbax Eclipse-XDB-C8, 5 µm, 150 x 4.6 mm i.d.<br />
with pre-column or equivalent<br />
Injection Volume: e.g. 50 µL<br />
Oven temperature: 40°C<br />
Mobile Phase: Bin Pump A: Water/acetonitrile (9/1, v/v), containing 0.22 mL/L formic<br />
acid)<br />
Bin Pump B: Acetonitrile, containing 0.22 mL/L formic acid<br />
Iso Pump C: Water/acetonitrile (1/1, v/v), containing 0.1 mL/L acetic acid<br />
or formic acid)<br />
Time Table:<br />
Time [min] A [%, v/v] B [%, v/v] Into MS Into Waste<br />
0.0 95 5 Iso pump Bin pump<br />
2.0 Bin pump Iso pump<br />
3.0 95 5<br />
3.1 10 90<br />
4.5 10 90<br />
4.6 95 5<br />
5.0 Iso pump Bin pump<br />
6.0 95 5<br />
6.0 Stop time<br />
Flow (Column): 1.0 mL/min<br />
Flow (into MS): 1.0 mL/min<br />
Retention times: BYI08330-enol-Glc approx. 4.6 min<br />
BYI08330-enol-Glc 13 C3 approx. 4.6 min
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