LMB Cambridge - Helsinki Institute of Physics
LMB Cambridge - Helsinki Institute of Physics
LMB Cambridge - Helsinki Institute of Physics
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Electron Cryo-Microscopy<br />
•Image molecules in native aqueous<br />
environment in vitreous ice (prevent de-hydration)<br />
•Trap important conformations in intermediate states <strong>of</strong> a<br />
kinetic cycle by rapid freezing : equivalent to time-resolved<br />
measurements<br />
•Images have low contrast : need sophisticated s<strong>of</strong>tware and<br />
lots <strong>of</strong> averaging<br />
•Radiation damage to specimen a severe limitation – hence<br />
need very good detectors!<br />
6/26/2008<br />
<strong>LMB</strong>, <strong>Cambridge</strong>
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