LMB Cambridge - Helsinki Institute of Physics

Single Particle Analysis
No crystals required .. Makes it possible, e.g. to analyse
structure of membrane proteins, which are very difficult to
crystalise,
Can be applied to large macromolecular complexes
• Powerful technique when used in conjunction with atomic
structures obtained with x-ray crystallography
•Applications: Virus particles, ribosomes, etc
•‘Best’ Resolution with this technique :Rotavirus capsid 3.8 Ǻ
•6.5 Million copies of the capsid protein used for the map
•From 8400 virus particles (excellent symmetry)
6/26/2008
LMB, Cambridge