Cell-based kinase assays in HTS Benjamin Bader

More documents

Recommendations

Info

Focussed medium throughput screen Distribution neutral controls: Distribution compounds / donor interference: • Neutral controls: similar distribution for both assays • Compounds: strong interference in BacMAM donor channel 26 HTRF-symposium 2013 261 26
Focussed medium throughput screen Lantha Assay common hits HTRF Assay interference 4686 cpds / 19% 24.228 interference 282 cpds / 1% Lantha HTRF Hits > 30% @ 10 µM (1.6%) 294 15 (0.3%) 63 available for retest 255 5 34 Interference Hit rate high (19%) high (1.6%) low (1%) low (0.3%) Confirmed hits (12%) confirmation rate (62%) 24 5 18 Confirmation rate low (12%) high (62%) available for IC50 28 3 11 (31%) confirmation rate (57%) IC50 hits 7 2 6 27 HTRF-symposium 2013 271 27
Page 1 and 2: Cell-based kinase assays in HTS Pot
Page 3 and 4: Kinases as drug targets cAbl and Gl
Page 5 and 6: Features of a typical early kinase
Page 7 and 8: General options for cell-based kina
Page 9 and 10: TR-FRET systems: HTRF and Lanthascr
Page 11 and 12: Introducing the PI3K / Akt / mTOR p
Page 13 and 14: Lanthascreen Assay Development BacM
Page 15 and 16: Lanthascreen - virus efficiency 30%
Page 17 and 18: Lanthascreen - optimization • sti
Page 19 and 20: Lanthascreen - inhibitor validation
Page 21 and 22: HTRF - cell lines 18000 Ratio 16000
Page 23 and 24: HTRF - pathway inhibitor validation
Page 25: Focussed medium throughput screen 2
Page 29 and 30: Acknowledgements Anja Kretzschmar S

Cell-based kinase assays in HTS Benjamin Bader

Create successful ePaper yourself

Delete template?

Save as template?