Cell-based kinase assays in HTS Benjamin Bader
Cell-based kinase assays in HTS Benjamin Bader
Cell-based kinase assays in HTS Benjamin Bader
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Requirements for efficient assay support <strong>in</strong> <strong>HTS</strong><br />
and Hit-to-lead phase @ BAYER<br />
Key objectives for every assay<br />
automated preparation of<br />
assay-ready plates<br />
(except HCA sta<strong>in</strong><strong>in</strong>g procedures)<br />
- assay-ready plates<br />
- 1536 well format, at least 384 well<br />
cells and reagent<br />
dispens<strong>in</strong>g <strong>in</strong>to 1536 well<br />
plates addition-only<br />
- homogenous addition only<br />
- endpo<strong>in</strong>t <strong>assays</strong><br />
- frozen cells<br />
- short<br />
- robust<br />
endpo<strong>in</strong>t detection<br />
with imag<strong>in</strong>g-<strong>based</strong><br />
multimode-readers<br />
6 HTRF-symposium 2013<br />
61<br />
6
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