Report No 1951/AP - Instytut Fizyki JÄdrowej PAN

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CH 3 78.2 O O 46.7 73.7 COOCH 3 B 76.4 A 59.1 N O 81.5 76.5 H 3 COOC O CH 3 85.2 I CH 3 O 70.1 B 48.8 N H O 88.7 59.9 COOCH 3 85.9 A 68.6 85.0 62.4 II COOCH 3 COOCH 3 Scheme 2. 1 J CC coupling constants in parent 9aH- (I) and 4H- (II) quinolizine 1,2,3,4- tetracarboxylate. Refereces: 1/ R. M. Acheson, G. A. Taylor, J. Chem. Soc., 1960, 1691 R. M. Acheson, R. S. Feinberg, J. M. F. Gagan, J. Chem. Soc., 1965, 948 14
NMR-BASED METHOD FOR THE LOCATION OF AMINO ACID MOIETIES IN BACTERIAL OLIGOSACCHARIDES Piotr Bernatowicz 1 , Andrzej Ejchart 1 , Gabriela Pastuch-Gawołek 2 , Wiesław Szeja 3 , Tomasz Lipiński 3 , and Andrzej Gamian 3 1 Laboratory of Biological NMR, IBB PAS, Warsaw, Poland 2 Department of Organic Chemistry, Biochemistry and Biotechnology, Silesian Technical University, Gliwice, Poland 3 Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, PAS, Wroclaw, Poland Structural variations of the core oligosaccharides of several species of Gram-negative bacteria, comprising substitution of amino acid glycine to the molecular backbone, have been recently reported. The genetic control of this process is not yet understood and the rationale behind glycine incorporation into the core oligosaccharides remains unclear, but one could expect that it increases the ability of the bacteria to cause host diseases. Therefore, an analytical method to identify the type of amino acid and to locate the site of its substitution is of importance. The model monosaccharides, ester-linked with an amino acid, were selected for the elaboration of an NMR-based method: Gly→4GlcOMe, Gly→6GalOMe, and Ala→6GalOMe. 1 H/ 13 C chemical shift correlation via long range coupling constant, 3 J(C'-O- C-H), was exploited for the detection of the covalent linkage between the amino acid residue and the saccharide moiety. The corresponding cross-peak at 13 C chemical shift characteristic for ester carbonyl {δ(C')≈165-170 ppm}appears in a relatively empty spectral region. Its 1 H chemical shift allows one to identify the substitution site providing the spectral assignments of saccharide protons have been performed. At the C' chemical shift one can also observe crosspeak to H and H protons of the amino acid residue thus identifying its type. It was found that such the HMBC-based NMR method is robust, efficient and reliable. 15
Page 1 and 2: The Henryk Niewodniczański INSTITU
Page 3 and 4: CONTENTS: DTI STUDY OF PATIENTS WIT
Page 5 and 6: DESIGN PRINCIPLES OF THE GRAPHICAL
Page 7 and 8: DO ZPV CORRECTIONS TO NMR SHIELDING
Page 9 and 10: FMRI IN ESTIMATION OF INFLUENCE OF
Page 11 and 12: DTI STUDY OF PATIENTS WITH SPONDYLO
Page 13: ONE-BOND CARBON-CARBON COUPLING CON
Page 17 and 18: T 1 AND T 1ρ PROTON RELAXATION TIM
Page 19 and 20: MAGNETIC ROTATIONAL RESONANCE AND C
Page 21 and 22: INTERACTION OF THE CAMPTOTHECIN FAM
Page 23 and 24: ARTIFACTS PRODUCED BY DENTAL MATERI
Page 25 and 26: “THROUGH SPACE” J-COUPLINGS OF
Page 27 and 28: DETERMINATION OF THE AMPLITUDE OF S
Page 29 and 30: NMR DIFFUSION STUDIES USING ULTRAHI
Page 31 and 32: NMR FINGERPRINT OF 2,5-DIHYDROXYPHE
Page 33 and 34: NMR OF HIGH-TEMPERATURE SUPERCONDUC
Page 35 and 36: EFFECT OF ANTIBIOTICS ON FORMING OF
Page 37 and 38: A SPECIALIZED PROBEHEAD FOR MR IMAG
Page 39 and 40: The free activation enthalpy ∆G
Page 41 and 42: motions of NH 3 in phases III and I
Page 43 and 44: at 200 K. Slow exchange limit is no
Page 45 and 46: APLICATION OF NMR SPECTROSCOPY FOR
Page 47 and 48: MOBILITY OF CD 4 MOLECULES IN NANOS
Page 49 and 50: NMR STUDY ON DIASTEREOMERIC DERIVAT
Page 51 and 52: 1 H NMR STUDIES OF POLY(ε-CAPROLAC
Page 53 and 54: Table 1. Phospholipids’ concentra
Page 55 and 56: BINARY MIXTURE OF HARD SPHERES AS A
Page 57 and 58: NMR STUDY OF Nd 2 Fe 14 BD x HYDRID
Page 59 and 60: The coefficient of dynamic viscosit
Page 61 and 62: FUNCTIONAL MAGNETIC RESONANCE IMAGI
Page 63 and 64: MOLECULAR MOTION IN ETHYLENE/NORBOR
Page 65 and 66:
TAUTOMERISM AND HYDROGEN BONDING OF
Page 67 and 68:
For the three 1 H nuclei in the met
Page 69 and 70:
UNIQUE INFORMATION ABOUT MOLECULAR
Page 71 and 72:
NMR STUDY OF CMR EFFECT IN MANGANIT
Page 73 and 74:
NON-MARKOVIAN PROCESSES OF MOLECULA
Page 75 and 76:
ELECTRIC FIELD GRADIENTS IN MB 12 (
Page 77 and 78:
1 H- 13 C AND 1 H- 15 N NMR STUDIES
Page 79 and 80:
13 C CP-MAS NMR STUDIES OF MIANSERI
Page 81 and 82:
NMR STUDY OF GdFe 2 H x HYDRIDES Vi
Page 83 and 84:
THEORY OF DAMPED QUANTUM ROTATION I
Page 85 and 86:
APPLICATION OF MOLECULAR MODELING A
Page 87 and 88:
NMR STUDY OF ULTRAFINE POLYTETRAFLU
Page 89 and 90:
1 H NMR DETECTION OF σ-ADDUCTS IN
Page 91 and 92:
[4] M. Tanasiewicz, W.P. Węglarz,
Page 93 and 94:
1 H MAS AND 13 C CP/MAS NMR STUDIES
Page 95 and 96:
ESTIMATION OF SPEECH REGIONS IN BIL
Page 97 and 98:
NEURAL CORRELATES OF WORKING MEMORY
Page 99 and 100:
APPLICATION OF 13 C-NMR TO THE STUD
Page 101 and 102:
DIAGNOSTIC IMPORTANCE OF MAGNETIZAT
Page 103 and 104:
13 C CP MAS STUDIES OF BAICALEIN AN
Page 105 and 106:
ORDERING EFFECTS IN COMPUTER-SIMULA
Page 107 and 108:
mainly phosphocholine and phosphoet
Page 109 and 110:
References: 1. Kasyan L.I. Tarabara
Page 111 and 112:
of Mo (Re) ions, which in turn is p
Page 113 and 114:
THREE-DIMENSIONAL STRUCTURE AND BAC
Page 115 and 116:
MRI STUDIES OF TABLETS Monika Zieli
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Report No 1951/AP - Instytut Fizyki JÄ drowej PAN

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Report No 1951/AP - Instytut Fizyki JÄdrowej PAN