Report No 1951/AP - Instytut Fizyki JÄ drowej PAN

PROTON NMR STUDIES OF HARD DENTAL TISSUES UNDER FAST MAS
Joanna Kolmas 1 , Zofia Paszkiewicz 2 , Anna Slosarczyk 2 , and Waclaw Kołodziejski 1
1 Department of Inorganic and Analytical Chemistry, Medical University of Warsaw,
ul. Banacha 1, 02-097 Warszawa, Poland.
2 Stanislaw Staszic Univ., Min. And Mat. Faculty of Materials Science and Ceramics,
Al. Mickiewicza 30, Kraków 30-059, Poland.
Enamel, dentin and dental cementum were studied by proton solid-state NMR with
magic-angle spinning (MAS). The experiments were done at 400 MHz using the Bloch decay
pulse sequence and the MAS rate at 30 kHz. Collagen type I, carbonate apatite and
hydroxyapatites calcined at various temperatures were used as principal organic and inorganic
standards for mineralized tissues. The proton lines were assigned and their shapes and
intensities were analysed. The signal of structural hydroxyl groups from enamel appeared at
ca. 0.0 ppm, as expected from the study of the mineral standards. However, this signal from
dentin and cementum was not clearly detectable. It was found possible to assess the water
content of apatites from proton spectra recorded with fast MAS.
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