Scientific CV in PDF format - IMP

BIOSKETCH & PUBLICATION LIST
Dr. Johannes Zuber, MD
DOB/POB
Citizenship
Position
Contact information
Education
September 1, 1974 in Dresden
German
Group Leader (PI), Research Institute of Molecular Pathology (IMP)
Research Institute of Molecular Pathology (IMP)
Dr. Bohr-Gasse 7, 1030 Vienna, Austria
Office phone: +43-1-79 730 3410, Mobile: +43-676-496 7199
Email: zuber@imp.ac.at
Website: http://www.imp.ac.at/research/johannes-zuber
1994 - 2001 Medical School Charité, Humboldt University of Berlin, Germany
4/2000 Medical board (Staatsexamen), grade 1.0 (1=best, 6=bottom)
5/2001 Medical graduation (Ärztliche Prüfung), grade 1.16 (1=best, 6=bottom)
4/2003 Definite physician license (Approbation als Arzt)
1998 - 2003 Doctoral Thesis; Department of Molecular Tumor Pathology, Charité, Humboldt University
of Berlin; Laboratory of Reinhold Schäfer (PhD)
Project title: “A genome-wide survey of Ras transformation targets”
9/2003 Dr. med. (Doktor der Medizin), Humboldt-University of Berlin, Germany
Final grade: summa cum laude
Positions and Appointments
2001 - 2005 Clinical Residency (Arzt im Praktikum & Assistenzarzt) in Hematology and Oncology,
Charité/ Virchow-Klinikum University Hospital, Berlin, Germany
Clinical training in general oncology, hematology and hemostaseology, high-dose chemotherapy
and stem cell transplantation, intensive care medicine.
2005 - 2008 Postdoctoral Research Fellow, Laboratory of Scott W. Lowe (PhD), Cold Spring Harbor
Laboratory, Cold Spring Harbor, NY, USA
2009 - 2010 Clinical Fellow, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
Main research interests: Development of in-vivo RNAi technologies and cancer mouse models.
Multiplexed negative selection RNAi screening to identify and evaluate therapeutic targets.
Chromatin modifier genes in progression and maintenance of AML and other cancers.
since 2011
Fellowships and Awards
Group Leader (PI) at the Research Institute of Molecular Pathology (IMP), Vienna
Biocenter, Vienna, Austria
1994 - 2001 German Scholarship Foundation (Studienstiftung des Deutschen Volkes)
1998 - 2000 Charité Research Fellowship
2006 - 2008 Research Fellowship of the DFG
2006 - 2007 Ambassador Felix Schnyder Grant (Lauri Strauss Leukemia Foundation)
2008 Scholar-in-training Award der AACR
Keystone Symposia Scholarship
2009 - 2010 Andrew Seligson Memorial Clinical Fellowship

Publications (in reverse chronological order; *indicates equal contribution)
2011-2012
Dow, L.E.*, Premsrirut, P.K.*, Zuber, J.*, Fellmann, C., McJunkin, K., Miething, C., Park, Y., Dickins, R.A., Hannon, G.J., and
Lowe, S.W. (2012). A pipeline for the generation of shRNA transgenic mice. Nature Protocols 7, 374-393.
Glaser, S.P., Lee, E.F., Trounson, E., Bouillet, P., Wei, A., Fairlie, W.D., Izon, D.J., Zuber, J., Rappaport, A.R., Herold, M.J., Alexander,
W.S., Lowe, S.W., Robb, L., and Strasser, A. (2012). Anti-apoptotic Mcl-1 is essential for the development and sustained
growth of acute myeloid leukemia. Genes Dev 26, 120-125.
Elling, U., Taubenschmid, J., Wirnsberger, G., O'Malley, R., Demers, S.P., Vanhaelen, Q., Shukalyuk, A.I., Schmauss, G.,
Schramek, D., Schnuetgen, F., von Melchner, H., Ecker, J.R., Stanford, W.L., Zuber, J., Stark, A., and Penninger, J.M. (2011).
Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. Cell Stem Cell 9, 563-574.
Zuber, J.*, Shi, J.*, Wang, E., Rappaport, A.R., Herrmann, H., Sison, E.A., Magoon, D., Qi, J., Blatt, K., Wunderlich, M., Taylor,
M.J., Johns, C., Chicas, A., Mulloy, J.C., Kogan, S.C., Brown, P., Valent, P., Bradner, J.E., Lowe, S.W., and Vakoc, C.R. (2011). RNAi
screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia. Nature 478, 524-528.
Zuber, J.*, Rappaport, AR.*, Luo, W., Wang, E., Chen, C., Vaseva, AV., Shi, J., Weissmueller, S., Fellmann, C., Taylor, MJ.,
Graeber, TG., Kogan, S.C., Vakoc, CR., Lowe, SW. (2011). An integrated approach to dissecting oncogene addiction implicates
a Myb coordinated self-renewal program as essential for leukemia maintenance Genes Dev 25(15):1628-40
Fellmann, C.*, Zuber, J.*, McJunkin, K., Chang, K., Malone, CD., Dickins, RA., Xu, Q., Hengartner, MO., Elledge, SJ., Hannon,
GJ., Lowe, SW. (2011). Functional identification of optimized RNAi triggers using a massively parallel sensor assay. Molecular
Cell 41(6):733-46
McJunkin, K., Mazurek, A., Premsrirut, PK., Zuber, J., Dow, LE., Simon, J., Stillman, B., Lowe, SW. (2011). Reversible suppression
of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A 108(17):7113-8
Pardee, TS., Zuber, J., Lowe, SW. (2011). Flt3-ITD alters chemotherapy response in vitro and in vivo in a p53-dependent
manner. Exp Hematol. 39(4):473-485.e4
Premsrirut, PK.*, Dow, LE.*, Kim, SY., Camiolo, M., Malone, CD., Miething, C., Scuoppo, C., Zuber, J., Dickins, RA., Kogan, SC.,
Shroyer, KR., Sordella, R., Hannon, GJ., Lowe, SW. (2011). A rapid and scalable system for studying gene function in mice using
conditional RNA interference. Cell 145(1):145-58
Zuber, J.*, McJunkin, K.*, Fellmann, C., Dow, LE., Taylor, MJ., Hannon, GJ., Lowe, SW. (2011). Toolkit for evaluating genes required
for proliferation and survival using tetracycline-regulated RNAi. Nature Biotechnology 29(1):79-83
2007-2010
Mavrakis, KJ., Wolfe, AL., Oricchio, E., Palomero, T., de Keersmaecker, K., McJunkin, K., Zuber, J., James, T., Khan, AA., Leslie,
CS., Parker, JS., Paddison, PJ., Tam, W., Ferrando, A., Wendel, HG. (2010). Genome-wide RNA-mediated interference screen
identifies miR-19 targets in Notch-induced T-cell acute lymphoblastic leukaemia. Nature Cell Biology 12(4):372-9
Zhao, Z.*, Zuber, J.*, Diaz-Flores, E., Lintault, L., Kogan, SC., Shannon, K., Lowe, SW. (2010). p53 loss promotes acute myeloid
leukemia by enabling aberrant self-renewal. Genes Dev 24(13):1389-402
Blobel, GA., Kadauke, S., Wang, E., Lau, AW., Zuber, J., Chou, MM., Vakoc, CR. (2009). A reconfigured pattern of MLL occupancy
within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit. Molecular Cell 36(6):970-
83
Bric, A.*, Miething, C.*, Bialucha, CU.*, Scuoppo, C., Zender, L., Krasnitz, A., Xuan, Z., Zuber, J., Wigler, M., Hicks, J., McCombie,
RW., Hemann, MT., Hannon, GJ., Powers, S., Lowe, SW. (2009). Functional identification of tumor-suppressor genes through
an in vivo RNA interference screen in a mouse lymphoma model. Cancer Cell 16(4):324-35
Guo, X., Keyes, WM., Papazoglu, C., Zuber, J., Li, W., Lowe, SW., Vogel, H., Mills, AA. (2009). TAp63 induces senescence and
suppresses tumorigenesis in vivo. Nat Cell Biology. 11(12):1451-7
Zuber, J., Radtke, I., Pardee, TS., Zhao, Z., Rappaport, AR., Luo, W., McCurrach, ME., Yang, MM., Kogan, SC., Downing, JR.,
Lowe, SW. (2009). Mouse models of human AML accurately predict chemotherapy response. Genes Dev 23(7):877-89
Zender, L.*, Xue, W.*, Zuber, J., Semighini, CP., Krasnitz, A., Ma, B., Zender, P., Kubicka, S., Luk, JM., Schirmacher, P.,
McCombie, WR., Wigler, M., Hicks, J., Hannon, GJ., Powers, S., Lowe, SW. (2008). An oncogenomics-based in vivo RNAi screen
identifies tumor suppressors in liver cancer. Cell 135(5):852-64
Zender, L., Zuber, J., Lowe, SW. (2007). Snapshot: genetic mouse models of cancer. Cell 129(4):838

Before 2005
Tchernitsa, OI., Sers, C., Zuber, J., Hinzmann, B., Grips, M., Schramme, A., Lund, P., Schwendel, A., Rosenthal, A., Schäfer, R.
(2004). Transcriptional basis of KRAS oncogene-mediated cellular transformation in ovarian epithelial cells. Oncogene
23(26):4536-55
Trappe, RU., Riess, H., Lippek, F., Plotkin, M., Schumacher, V., Royer-Pokora, B., Hildebrandt, B., Zuber, J., Mapara, MY., Oertel,
S., Dörken, B. (2004). Effective use of high-dose chemotherapy and autologous stem cell rescue for relapsed adult Wilms'
tumor and a novel alteration in intron 1 of the WT1 gene. J Pediatr Hematol Oncol 26(12):820-3
Baskaynak, G., Kreuzer, KA., Schwarz, M., Zuber, J., Audring, H., Riess, H., Dörken, B., le Coutre, P. (2003). Squamous cutaneous
epithelial cell carcinoma in two CML patients with progressive disease under imatinib treatment. Eur J Haematol
70(4):231-4
Schäfer, R., Tchernitsa, OI., Zuber, J., Sers, C. (2003). Dissection of signal-regulated transcriptional modules by signaling
pathway interference in oncogene-transformed cells. Adv Enzyme Regul 43:379-91
Sers, C., Tchernitsa, OI., Zuber, J., Diatchenko, L., Zhumabayeva, B., Desai, S., Htun, S., Hyder, K., Wiechen, K., Agoulnik, A.,
Scharff, KM., Siebert, PD., Schäfer, R. (2002). Gene expression profiling in RAS oncogene-transformed cell lines and in solid
tumors using subtractive suppression hybridization and cDNA arrays. Adv Enzyme Regul 42:63-82
Zuber, J.*, Tchernitsa, OI.*, Hinzmann, B., Schmitz, AC., Grips, M., Hellriegel, M., Sers, C., Rosenthal, A., Schäfer, R. (2000). A
genome-wide survey of RAS transformation targets. Nature Genetics 24(2):144-52
Tchernitsa, OI., Zuber, J., Sers, C., Brinckmann, R., Britsch, SK., Adams, V., Schäfer, R. (1999). Gene expression profiling of fibroblasts
resistant toward oncogene-mediated transformation reveals preferential transcription of negative growth regulators.
Oncogene 18(39):5448-54
Selected Presentations
2007 Keystone Meeting: Mouse Models at the Frontiers of Cancer Discovery. Whistler, Canada, poster presentation
Aspen Cancer Conference 2007, Aspen, USA, poster
2008 Mouse-Models of Human Cancer, Washington, D.C., USA, talk
AACR, San Diego, USA, poster
Mechanisms & Models of Cancer, Cold Spring Harbor, NY, USA, talk
2009 Keystone Meeting: Targeted Cancer Therapies, Whistler, Canada, talk and poster presentation
2010 Aspen Cancer Conference 2010, Aspen, USA, poster
Mechanisms & Models of Cancer, Cold Spring Harbor, NY, USA, talk
2011 6 th Microsymposium on Small RNAs, Vienna, Austria, talk
EMBO Workshop Synthetic Lethality, Vienna, Austria, talk
Working Conference on Cancer Stem Cells 2011, Vienna, Austria, talk
ISREC Symposium 2011, Hallmarks and Horizons of Cancer, Lausanne, Switzerland, talk
OeGMBT Annual Meeting 2011, Salzburg, Austria, talk
2012 Karolinska Institutet, Stockholm, Sweden, invited seminar
Boehringer International Biology Meeting, Vienna, Austria, talk
CRG, Barcelona, Spain, invited seminar
Comprehensive Cancer Center Mainfranken, Marburg/Würzburg, Germany, invited seminars
AACR Educational Session, Chicago, USA, talk
DFG Hinterzartener Kreis Annual Meeting, Como, Italy, talk
EMBL, Monterotondo, Italy, invited seminar
NKI, Amsterdam, Netherlands, invited seminar
Patents
2001 Nachweis von differenzieller Genexpression (PCT/EP2001/001003)
2009 High Throughput Methods for Functionally Determining RNA Interference Efficiency (PCT/US2008/081193)
2010 Identification of RNAi Targets and Use of RNAi for Rational Therapy of Chemotherapy-Resistant Leukemia
and Other Cancers (PCT/US2010/029083)
2011 Compositions and Methods for Treating Leukemia (PCT/US2011/036672)