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PROCEEDINGS OF THE FIFTH MOUNTAIN LION WORKSHOP 37 STANDARDIZING PHOTOGRAPHS OF PUMA TRACKS FOR DIGITAL PROCESSING Steve Galentine. Department <strong>of</strong> Wildlife, Fish, and Conservation Biology, University <strong>of</strong> California, Davis, California 95616. E.L. Fitzhugh. Cooperative Extension, Department <strong>of</strong> Wildlife, Fish, and Conservation Biology, University <strong>of</strong> California, Davis, California 95616. Key words: Mountain Lion, Puma concolor, Tracks, Techniques Abstract We designed a portable camera mount that provides photographs <strong>of</strong> animal tracks, taken perpendicular to <strong>the</strong> substrate, <strong>of</strong> consistent quality and size. The camera sits on a folding black box. Opposing flash units are mounted at different levels depending on <strong>the</strong> depth <strong>of</strong> <strong>the</strong> track. We identified appropriate filters for <strong>the</strong> film, aperture, and flash units we used, although <strong>the</strong>se will vary and may be improved upon. Scientific attention has been directed recently toward using animal tracks to 1) identify species, 2) index populations, and 3) identify individual animals. One aspect <strong>of</strong> some <strong>of</strong> <strong>the</strong>se techniques involves computerized measurement <strong>of</strong> track dimensions and areas. To accomplish computer measurements, <strong>the</strong> track outlines and o<strong>the</strong>r features must first be identified (digitized) for <strong>the</strong> computer. Tracks traced on acetate or plastic film are relatively easily and accurately digitized, but tracing <strong>the</strong> tracks in <strong>the</strong> field requires valuable field time and <strong>the</strong> tracings may be distorted by parallax. Parallax is caused by light rays that bend when <strong>the</strong>y pass from <strong>the</strong> glass plate on which <strong>the</strong> tracing is made to <strong>the</strong> air above and below <strong>the</strong> plate. If <strong>the</strong> eye <strong>of</strong> <strong>the</strong> tracer is not perpendicular to <strong>the</strong> glass at <strong>the</strong> exact spot being traced, this bending, or parallax, occurs and distorts <strong>the</strong> image. A similar error can exist in photographs not taken from a perpendicular perspective. Photographs greatly reduce field time, and can eliminate <strong>the</strong> parallax error if <strong>the</strong>y are taken from a location perpendicular to <strong>the</strong> substrate <strong>the</strong> track is on (not necessarily vertical). Photographs, however, must be digitized in <strong>the</strong> <strong>of</strong>fice. If <strong>the</strong> photographs, or <strong>the</strong>ir computer images, are <strong>of</strong> variable quality and size, <strong>the</strong> human error involved in identifying <strong>the</strong> true outlines and features may sometimes be greater than errors made by tracing in <strong>the</strong> field. Shallow tracks in light-colored substrates <strong>of</strong>ten lack detail in photographs, making <strong>the</strong> outline hard to identify. Deep tracks with side-lighting sometimes contain dark shadows on one side <strong>of</strong> <strong>the</strong> track just at <strong>the</strong> point where <strong>the</strong> vertical wall <strong>of</strong> <strong>the</strong> impression curves toward <strong>the</strong> horizontal bottom <strong>of</strong> <strong>the</strong> track. This area <strong>of</strong> curvature is where detail is needed to properly identify <strong>the</strong> outline <strong>of</strong> <strong>the</strong> track. The goal <strong>of</strong> this project is to develop a standardized apparatus and new techniques to enable researchers and wildlife managers to take <strong>the</strong> best possible photographs <strong>of</strong> <strong>mountain</strong> <strong>lion</strong> tracks. Substrate type and ambient light conditions are two <strong>of</strong> <strong>the</strong> major factors affecting <strong>the</strong> quality <strong>of</strong> photographs <strong>of</strong> tracks. Since we have no control over where an animal may walk and leave a track we focused our attention on <strong>the</strong> light factor to improve our photographs. To eliminate variation in this factor we developed <strong>the</strong> idea <strong>of</strong> a "black box" that would enable us to standardize light using camera flash units. We tested single and opposing flash units at various heights on different substrates and depths <strong>of</strong> tracks. We also experimented with flash filters, lens filters, and various camera settings. The result was a collapsible, portable camera mount, or "black box" designed to provide photographs <strong>of</strong> tracks taken perpendicular to <strong>the</strong> substrate, <strong>of</strong> consistent quality and size. METHODS Tests were done to learn proper exposure settings, filters, and number and location <strong>of</strong> flash units. Equipment Nikon F2 camera 35 mm lens 2 ea. Vivitar 2000 flash units Multi-flash adapter 2 ea flash cords 52 mm. Hoya circular polarizer PL-CIR 52 mm. Tiffen lens filter, red 1 52 mm. Tiffen lens filter, yellow 2(8) 52 mm. Tiffen lens filter, neutral density N.D. O6 Kodak Kodachrome 64 slide film Dark gray window tint, "Gila" non-reflective, 35% Dark Smoke (for flash filter) Procedures We first learned <strong>the</strong> shortest distance our equipment would focus. Using that as a guide, we constructed a collapsible 15-inch cubic box, with one side open. The opposite side had a circular hole in <strong>the</strong> center just large enough for <strong>the</strong> camera lens to fit through. We painted <strong>the</strong> box black to reduce light reflectance inside. We fitted <strong>the</strong> flash attachments through horizontal holes cut in <strong>the</strong> shape <strong>of</strong> <strong>the</strong> flash units. The bottoms <strong>of</strong> <strong>the</strong> holes were 2 inches, 4 inches, and 6 inches from <strong>the</strong> bottom <strong>of</strong> <strong>the</strong> box. These holes were directly opposite each o<strong>the</strong>r on two sides <strong>of</strong> <strong>the</strong> box.