Sphereclone (Phenomenex)
Sphereclone (Phenomenex)
Sphereclone (Phenomenex)
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SPHERECLONE <br />
Use SphereClone Columns<br />
as an Alternative to Spherisorb<br />
• Performance guaranteed<br />
SphereClone is packed only by <strong>Phenomenex</strong>, thereby reducing the<br />
chance of column-to-column variation. You will receive columns<br />
of consistent quality and reproducibility, and will benefit from<br />
<strong>Phenomenex</strong>’s excellent customer support and technical service.<br />
If SphereClone does not provide at least<br />
an equivalent separation as compared to<br />
Spherisorb columns of the same phase,<br />
particle size and comparable dimensions, send<br />
in your comparative data within 45 days and<br />
keep the SphereClone column for FREE.<br />
Below are listed side-by-side comparisons of physical material<br />
characteristics. As you can see, SphereClone and Spherisorb ®<br />
compare very well for important specifications such as particle<br />
size, pore size, surface area and carbon load.<br />
SphereClone vs. Spherisorb ®†<br />
SEM of Base Silica<br />
Material Characteristics<br />
SEM of Base Silica<br />
SphereClone vs. Spherisorb ®†<br />
3, 5, 10µ Particle Size 3, 5, 10µ<br />
80Å Pore Size 80Å<br />
HPLC<br />
200m 2 /g Surface Area 200m 2 /g<br />
Carbon Load<br />
SphereClone<br />
Surface<br />
15,000x Magnification<br />
— Silica —<br />
6% C6 6%<br />
6% C8 6%<br />
7% ODS(1) 7%<br />
12% ODS(2) 12%<br />
Surface<br />
15,000x Magnification<br />
2% NH 2<br />
2%<br />
3.0% Phenyl 3.0%<br />
Physical Mass<br />
Distribution & Shape<br />
4,000x Magnification<br />
— SCX —<br />
Physical Mass<br />
Distribution & Shape<br />
4,000x Magnification<br />
Particle Size Distribution<br />
vs.<br />
SphereClone 5µ Silica<br />
Spherisorb ® 5µ Silica<br />
SphereClone<br />
Reproducibility<br />
Value<br />
QC Data for Silica<br />
Surface Area (m 2 /g)<br />
Pore Volume (mL/g)<br />
Pore Diameter (Å)<br />
Standard Deviation<br />
Particle Diameter<br />
5µ Particle Size Data<br />
Dp number<br />
Dp volume<br />
STD<br />
Batch<br />
Batch<br />
226 <strong>Phenomenex</strong>
SPHERECLONE <br />
SphereClone <br />
‡<br />
vs.<br />
Spherisorb ®†<br />
5µ ODS(2) 5µ ODS(2)<br />
App ID 5548<br />
mAU<br />
800<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
5µ ODS(2)<br />
6<br />
4<br />
7<br />
5<br />
3<br />
App ID 5549<br />
mAU<br />
800<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
6<br />
4+5<br />
5µ ODS(2)<br />
7<br />
3<br />
Fat-Soluble Vitamins<br />
Dimensions: 150 x 4.6mm<br />
Mobile Phase: Acetonitrile/Methanol (75:25)<br />
Flow Rate: 1.3mL/min<br />
Detection: UV @ 280nm<br />
Sample: 1. Vitamin A<br />
2. Vitamin A Acetate<br />
3. Vitamin D 2<br />
4. Vitamin D 3<br />
5. Vitamin E<br />
6. Vitamin E Acetate<br />
7. Vitamin K 1<br />
App ID 5550<br />
mAU<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0 5 10 min<br />
3<br />
1<br />
2<br />
5µ ODS(2)<br />
4<br />
5<br />
App ID 5551<br />
mAU<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0 5 10<br />
1<br />
2<br />
3<br />
4<br />
5<br />
min<br />
5µ ODS(2)<br />
Pesticides/Herbicides<br />
Dimensions: 150 x 4.6mm<br />
Mobile Phase: Water/Acetonitrile (70:30)<br />
Flow Rate: 1.5mL/min<br />
Detection: UV @ 220nm<br />
Sample: 1. Baygon <br />
2. Carbofuran<br />
3. Carbaryl<br />
4. Propham<br />
5. Captan<br />
HPLC<br />
SphereClone<br />
0<br />
0<br />
App ID 5558<br />
mAU<br />
160<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
0 10 20 30 min<br />
1<br />
2<br />
‡<br />
vs.<br />
0 10 20 30 min<br />
5µ ODS(1) 5µ ODS(1) +<br />
3<br />
5 m ODS(1)<br />
4<br />
App ID 5559<br />
mAU<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
2<br />
3<br />
1 5 m ODS(1)<br />
4<br />
+<br />
Polar Compounds<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: 25mM KH 2<br />
PO 4<br />
, pH 2.5<br />
Flow Rate: 1.0mL/min<br />
Temperature: 40°C<br />
Detection: UV @ 230nm<br />
Sample: 1. L-Cysteine<br />
2. L-Ascorbic Acid<br />
3. Glutathione<br />
4. Uric Acid<br />
0 2 4 6 8<br />
min<br />
0 2 4 6 8<br />
min<br />
App ID 5562<br />
mAU<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
1<br />
2<br />
3<br />
4<br />
5 m ODS(1)<br />
App ID 5563<br />
mAU<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
1<br />
2<br />
3<br />
5 m ODS(1)<br />
4<br />
+<br />
Phthalate<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: Acetonitrile/Water (70:30)<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 230nm<br />
Sample: 1. Dimethyl Phthalate<br />
2. Anisole<br />
3. Naphthalene<br />
4. Dibutyl Phthalate<br />
0 2 4 6 8 10 min<br />
0 2 4 6 8 10 min<br />
<strong>Phenomenex</strong><br />
227
SPHERECLONE <br />
App ID 5572<br />
SphereClone Spherisorb ®†<br />
5 m C8<br />
mAU<br />
3<br />
350<br />
2<br />
4<br />
300<br />
250<br />
1<br />
200<br />
150<br />
100<br />
50<br />
‡<br />
vs.<br />
5µ C8 5µ C8 +<br />
App ID 5573<br />
3<br />
mAU<br />
350<br />
2<br />
4<br />
300<br />
250<br />
5 m C8 +<br />
200<br />
1<br />
150<br />
100<br />
50<br />
Polar Compounds<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: 25mM KH 2<br />
PO 4<br />
, pH 2.5<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 230nm<br />
Sample: 1. L-Cysteine<br />
2. L-Ascorbic Acid<br />
3. Glutathione<br />
4. Uric Acid<br />
0<br />
0<br />
0 2 4 6 8<br />
min<br />
0 2 4 6 8<br />
min<br />
HPLC<br />
SphereClone<br />
App ID 5576<br />
mAU<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
3<br />
4<br />
5<br />
5µ C8<br />
6<br />
App ID 5577<br />
mAU<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
4<br />
3<br />
5<br />
5µ C8<br />
6<br />
Water-Soluble Vitamins<br />
Dimensions: 150 x 4.6mm<br />
Mobile Phase: A: 5mM Sodium 1-Pentanesulfonate<br />
in 0.1% H 3<br />
PO 4<br />
B: 5mM Sodium 1- Pentanesulfonate in<br />
0.1% H 3<br />
PO 4<br />
in 80% Acetonitrile<br />
Gradient: A/B (97.5:2.5) to A/B (70:30) in 20 min<br />
Flow Rate: 1.0mL/min<br />
Temperature: 40°C<br />
Detection: UV @ 254nm<br />
Sample: 1. Niacinamide<br />
2. Pyridoxal<br />
3. p-Aminobenzoic Acid<br />
4. Thiamine<br />
5. Folic Acid<br />
6. Riboflavin<br />
0 5 10<br />
min<br />
0 5 10<br />
min<br />
App ID 5590<br />
mAU<br />
200<br />
175<br />
150<br />
125<br />
100<br />
75<br />
50<br />
25<br />
1<br />
2<br />
vs.<br />
‡<br />
mAU<br />
5µ C6 5µ C6<br />
200<br />
2<br />
1<br />
150<br />
5µ C6<br />
5µ C6<br />
100<br />
App ID 5591<br />
50<br />
Endorphins<br />
Dimensions:<br />
Mobile Phase:<br />
Gradient:<br />
Flow Rate:<br />
Detection:<br />
Sample:<br />
250 x 4.6mm<br />
A: 0.1% TFA in Water<br />
B: 0.1% TFA in Acetonitrile/Water<br />
(90:10)<br />
A:B-70:30 to A:B-55:45 in 15 min<br />
1.0mL/min<br />
UV @ 214nm<br />
1. Met Enkephalin<br />
2. Leu Enkephalin<br />
0<br />
0<br />
0 2 4 6 8 10 12<br />
min<br />
0 2 4 6 8 10 12 min<br />
App ID 5592<br />
mAU<br />
175<br />
150<br />
125<br />
100<br />
75<br />
50<br />
25<br />
0<br />
1<br />
2<br />
3<br />
4<br />
6<br />
7<br />
5µ SAX<br />
9<br />
8<br />
0 5 10 15 20 25 min<br />
5<br />
‡<br />
vs.<br />
5µ SAX 5µ SAX +<br />
App ID 5593<br />
mAU<br />
200<br />
175<br />
150<br />
125<br />
100<br />
75<br />
50<br />
25<br />
0<br />
1<br />
2<br />
3<br />
4<br />
5<br />
6<br />
9<br />
8<br />
5µ SAX +<br />
0 5 10 15 20 25 min<br />
7<br />
Nucleotides<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: A: 0.04M KH 2<br />
PO 4<br />
, pH 5.5<br />
B: 0.5M KH 2<br />
PO 4<br />
, pH 5.5<br />
Gradient: A/B: 100: 0 to A/B 0: 100 in 26 min<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 254nm<br />
Sample: 1. β-NAD<br />
2. IMP<br />
3. GMP<br />
4. AMP<br />
5. GDP<br />
6. ADP<br />
7. NADP<br />
8. ITP<br />
9. ATP<br />
228 <strong>Phenomenex</strong>
SPHERECLONE <br />
App ID 5596<br />
mAU<br />
1<br />
350<br />
2<br />
300<br />
5<br />
3 4<br />
250<br />
200<br />
6<br />
150<br />
100<br />
50<br />
‡<br />
vs.<br />
SphereClone Spherisorb ®†<br />
5µ NH 2<br />
5µ NH 2<br />
App ID 5597<br />
1<br />
mAU<br />
2<br />
300<br />
3 5<br />
4<br />
250<br />
200<br />
6<br />
150<br />
100<br />
50<br />
Corticosteroids<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: Dichloromethane/Methanol (95:5)<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 254nm<br />
Sample: 1. Deoxycorticosterone Acetate<br />
2. Deoxycorticosterone<br />
3. Hydrocortisone 21-Acetate<br />
4. Corticosterone<br />
5. Cortisone<br />
6. Hydrocortisone<br />
0<br />
0<br />
App ID 5602<br />
mAU<br />
175<br />
150<br />
125<br />
100<br />
75<br />
50<br />
25<br />
0<br />
0 2 4 6 8<br />
1 2<br />
5<br />
3 4<br />
6<br />
7<br />
8<br />
min<br />
‡<br />
vs.<br />
App ID 5603<br />
mAU<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
0 2 4 6 8<br />
5µ Phenyl 5µ Phenyl +<br />
1<br />
2<br />
3<br />
4<br />
5<br />
7<br />
6<br />
min<br />
8<br />
Aromatic Ketones<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: Acetonitrile/Water (33:67)<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 254nm<br />
Sample: 1. Benzamide<br />
2. Benzyl Alcohol<br />
3. Acetophenone<br />
4. Methyl Benzoate<br />
5. Phenetole<br />
6. Naphthalene<br />
7. Benzophenone<br />
8. Biphenyl<br />
HPLC<br />
SphereClone<br />
1 5 10 15 20 min<br />
0 5 10 15 20 25 min<br />
ORDERING INFORMATION<br />
SecurityGuard Cartridges require universal holder Order No.: KJO-4282<br />
3µ Columns (mm) SecurityGuard Cartridges<br />
150 x 1.0 100 x 2.0 150 x 2.0 100 x 3.2 150 x 3.2 100 x 4.6 150 x 4.6 4 x 2.0mm 4 x 3.0mm<br />
Phases<br />
/10pk /10pk<br />
Silica I — I — I — I — I — I 00D-4130-E0 I 00F-4130-E0 AJO-4347 I AJO-4348<br />
C6 — — 00F-4132-B0 — — 00D-4132-E0 00F-4132-E0 — —<br />
C8 — — — 00D-4133-R0 — 00D-4133-E0 — AJO-4289 AJO-4290<br />
ODS (1) — 00D-4134-B0 00F-4134-B0 00D-4134-R0 00F-4134-R0 00D-4134-E0 00F-4134-E0 AJO-4286 AJO-4287<br />
ODS(2) 00F-4135-A0 00D-4135-B0 00F-4135-B0 00D-4135-R0 00F-4135-R0 00D-4135-E0 00F-4135-E0 AJO-4286 AJO-4287<br />
NH 2<br />
— — — — 00F-4137-R0 — 00F-4137-E0 AJO-4301 AJO-4302<br />
Phenyl — — — — — 00D-4138-E0 00F-4138-E0 AJO-4350 AJO-4351<br />
for ID: 2.0-3.0mm 3.2-8.0mm<br />
5µ Columns (mm) SecurityGuard Cartridges<br />
250 x 2.0 150 x 3.2 250 x 3.2 150 x 4.6 250 x 4.6 250 x 10.0 250 x 21.2 4 x 2.0mm 4 x 3.0mm 10 x 10mm<br />
Phases<br />
Silica I 00G-4139-B0 I /10pk /10pk /3pk<br />
— I — I 00F-4139-E0 I 00G-4139-E0 I — I — AJO-4347 I AJO-4348 I AJO-7223<br />
C6 — — — 00F-4141-E0 00G-4141-E0 00G-4141-N0 — — — —<br />
C8 00G-4142-B0 00F-4142-R0 00G-4142-R0 00F-4142-E0 00G-4142-E0 00G-4142-N0 — AJO-4289 AJO-4290 AJO-7222<br />
ODS (1) 00G-4143-B0 00F-4143-R0 00G-4143-R0 00F-4143-E0 00G-4143-E0 00G-4143-N0 — AJO-4286 AJO-4287 AJO-7221<br />
ODS (2) 00G-4144-B0 00F-4144-R0 00G-4144-R0 00F-4144-E0 00G-4144-E0 00G-4144-N0 00G-4144-P0 AJO-4286 AJO-4287 AJO-7221<br />
NH 2<br />
— — 00G-4147-R0 00F-4147-E0 00G-4147-E0 00G-4147-N0 — AJO-4301 AJO-4302 AJO-7364<br />
Phenyl — — — 00F-4148-E0 00G-4148-E0 — — AJO-4350 AJO-4351 AJO-7314<br />
SAX 00G-4149-B0 00F-4149-R0 00G-4149-R0 00F-4149-E0 00G-4149-E0 00G-4149-N0 — AJO-4310 AJO-4311 AJO-7370<br />
for ID: 2.0-3.0mm 3.2-8.0mm 9-16mm<br />
10µ Columns (mm) SecurityGuard Cartridges<br />
250 x 4.6 4 x 3.0mm<br />
‡<br />
Comparative separations may not be representative of all applications.<br />
SphereClone is a trademark of <strong>Phenomenex</strong>.<br />
Phases<br />
/10pk<br />
<strong>Phenomenex</strong> is a registered trademark.<br />
+<br />
C8 I 00G-4154-E0 AJO-4290<br />
Spherisorb columns used for comparison studies were<br />
ODS (1)<br />
ODS (2)<br />
00G-4155-E0<br />
00G-4156-E0<br />
AJO-4287<br />
AJO-4287<br />
purchased from manufacturer.<br />
†<br />
Spherisorb is a registered trademark of Waters.<br />
<strong>Phenomenex</strong> has no affiliation with Waters.<br />
SAX 00G-4160-E0 AJO-4311<br />
for ID: 3.2-8.0mm<br />
See p. 213 for Semi-prep SecurityGuard Cartridge Holders<br />
C1, CN, SCX not currently available. See PhenoSphere on p. 193.<br />
Other dimensions from microbore/SFC to preparative columns<br />
available upon request.<br />
<strong>Phenomenex</strong><br />
229