Sphereclone (Phenomenex)
Sphereclone (Phenomenex)
Sphereclone (Phenomenex)
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SPHERECLONE <br />
SphereClone <br />
‡<br />
vs.<br />
Spherisorb ®†<br />
5µ ODS(2) 5µ ODS(2)<br />
App ID 5548<br />
mAU<br />
800<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
5µ ODS(2)<br />
6<br />
4<br />
7<br />
5<br />
3<br />
App ID 5549<br />
mAU<br />
800<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
1<br />
2<br />
6<br />
4+5<br />
5µ ODS(2)<br />
7<br />
3<br />
Fat-Soluble Vitamins<br />
Dimensions: 150 x 4.6mm<br />
Mobile Phase: Acetonitrile/Methanol (75:25)<br />
Flow Rate: 1.3mL/min<br />
Detection: UV @ 280nm<br />
Sample: 1. Vitamin A<br />
2. Vitamin A Acetate<br />
3. Vitamin D 2<br />
4. Vitamin D 3<br />
5. Vitamin E<br />
6. Vitamin E Acetate<br />
7. Vitamin K 1<br />
App ID 5550<br />
mAU<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0 5 10 min<br />
3<br />
1<br />
2<br />
5µ ODS(2)<br />
4<br />
5<br />
App ID 5551<br />
mAU<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0 5 10<br />
1<br />
2<br />
3<br />
4<br />
5<br />
min<br />
5µ ODS(2)<br />
Pesticides/Herbicides<br />
Dimensions: 150 x 4.6mm<br />
Mobile Phase: Water/Acetonitrile (70:30)<br />
Flow Rate: 1.5mL/min<br />
Detection: UV @ 220nm<br />
Sample: 1. Baygon <br />
2. Carbofuran<br />
3. Carbaryl<br />
4. Propham<br />
5. Captan<br />
HPLC<br />
SphereClone<br />
0<br />
0<br />
App ID 5558<br />
mAU<br />
160<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
0 10 20 30 min<br />
1<br />
2<br />
‡<br />
vs.<br />
0 10 20 30 min<br />
5µ ODS(1) 5µ ODS(1) +<br />
3<br />
5 m ODS(1)<br />
4<br />
App ID 5559<br />
mAU<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
2<br />
3<br />
1 5 m ODS(1)<br />
4<br />
+<br />
Polar Compounds<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: 25mM KH 2<br />
PO 4<br />
, pH 2.5<br />
Flow Rate: 1.0mL/min<br />
Temperature: 40°C<br />
Detection: UV @ 230nm<br />
Sample: 1. L-Cysteine<br />
2. L-Ascorbic Acid<br />
3. Glutathione<br />
4. Uric Acid<br />
0 2 4 6 8<br />
min<br />
0 2 4 6 8<br />
min<br />
App ID 5562<br />
mAU<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
1<br />
2<br />
3<br />
4<br />
5 m ODS(1)<br />
App ID 5563<br />
mAU<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
1<br />
2<br />
3<br />
5 m ODS(1)<br />
4<br />
+<br />
Phthalate<br />
Dimensions: 250 x 4.6mm<br />
Mobile Phase: Acetonitrile/Water (70:30)<br />
Flow Rate: 1.0mL/min<br />
Detection: UV @ 230nm<br />
Sample: 1. Dimethyl Phthalate<br />
2. Anisole<br />
3. Naphthalene<br />
4. Dibutyl Phthalate<br />
0 2 4 6 8 10 min<br />
0 2 4 6 8 10 min<br />
<strong>Phenomenex</strong><br />
227
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